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Insulin Production_ Enzyme Reactor

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Insulin Production_ Enzyme Reactor Powered By Docstoc
					   Chris Istchenko
Allan Vandervoort
       Sanath Rao
    Aamir Ahmad
Outline
• Reactor Inlets
• Internal Reaction Process
• Reaction Kinetics and Sizing
• Pricing
• Alternatives
Reactor Inlets
• Single Chain Insulin Precursor
• Enzyme (Trypsin)
• Organic Solvent (THF)
• Threonine ester (Thr-OMe)
• Water for Injection (10-40%)
Internal Reaction Process
 Transpeptidation Reaction
 Reaction at Lysine sites but not Arginine
 Organic solvent reverses proteolytic
  reactions and reduces arginine reactions
 Transpeptidation with threonine ester
Enzyme Reactors
 Batch Stirred Tank
    Reactor
   Batch Membrane
    Reactor
   Packed Bed
    Reactor
   Continuous Flow
    Stirred Tank
    Reactor
   Continuous Flow Membrane Reactor
   Fluidized Bed Reactor
Enzyme Kinetics
 Michaelis-Menten Kinetics
 Rate described as



 Batch Reactor Design Equation



 km=0.1 M & Vmax=0.018 M/h
Results
 Initial mass of proinsulin = 30.45 kg (Superpro)
 Conversion of 75%
 Time = 4 hours
 Reactor Volume = 77 L
   12 ˚C
 pH 6.5
Costing
 Batch STR Tank
    Supplier located (Mueller)
    Cost estimate of $20 604
 Proinsulin required=
  30.45 kg
 USP Grade Trypsin
    1:200 Proinsulin by weight
 Threonine Ester
    5:1 Proinsulin by moles
Costing Cont.
                                                         Cost
Material    Cost ($) Amount (g) Amount Required (g)
                                                      ($/batch)
 Trypsin    434.10     10              203              6 169
Threonine
             63.50      5              3400            29 685
  Ester
Alternatives
 Continuous reactor
   Lower operating costs
   Need for whole process continuity
 Alternative organism (Achromobucter lyticus)
   Shows high yield
   Need to investigate kinetics
 Other modifications to insulin

				
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posted:12/11/2012
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