Effects of Androgens on Pancreatic KATP Channels Action
Mehrdad Kassaee, Rahim Ahmadi
Faculty of Science, Islamic Azad University, Hamedan Branch, Hamedan,
Background and Aims: Verapamil is the prototype phenylalkylamine KATP
channel inhibitor which rises insulin secretion by direct inhibiting of KATP
channels in pancreatic B-cells. On the other hand, the hormone testosterone
can influence insulin secretion, however, the results in this field are some
conflicting and confusing. This study was done to know whether testosterone
can influence KATP channels in pancreatic B-cells in rats.
Materials and Methods: Verapamil (100 mg/kg/day) was administered in
drinking water. Testosterone enantate (10 mg/kg/day) was injected
intraperiotenally. After 4 weeks, blood samples were collected and serum
insulin was measured.
Results: verapamil, testosterone and "verapamil+testosterone" administration
resulted in increasing of insulin secretion in rats (P<0.01, P<0.05 and
P<0.001, respectively); however, there was no significant difference between
serum insulin level of these 3 groups.
Conclusion: The findings of our study indicated that the effect of verapamil
on insulin secretion is not influenced by normal dose of testosterone
administration, i.e., presumably normally testosterone effect on pancreatic B-
cells is not modulated through KATP channels in pancreatic B-cells.
Key words: Pancreatic KATP channel, Testosterone