HPLC port

Document Sample
HPLC port Powered By Docstoc
					      Schuyler Mincemoyer

                                     High Performance Liquid Chromatography


                                                                                                                 To Waste
      Block Diagram:

 Regulated                                                                                    Pulse Damper
 Helium Source                                                       Output
                                                                     Check Valve



                                                              Pump                                           Drain
                                                                                                             Valve

                                                                         Inlet Check
                                                                         Valve

                                               Inlet
                 Solvent        Sparger        Filter
                 Reservoirs
                                                                                             Priming
                                                                                             Syringe


          Solvent
          Proportioning
          Valve                                         Pressure
                                                        Transducer
                                                                             Back-Pressure             Filter
        Column                Injector Valve
                                                                             Regulator



To Detector

      Components

      Solvent Reservoir: Holds 500mL or more of a solvent.

      Sparger: dissolves gases that are swept out of solution by fine bubbles of an inert gas that is not soluble in the
      mobile phase.

      Pump: There are generally two types of pumps (reciprocating and displacement), but are used to pump the
      liquids. Needs to generate pressure up to 6000 psi, have pulse free output, flow rates ranging from 0.1-
      10mL/min and resistance to corrosion by a variety of solvents.

      Pulse Damper: Used to remove any pulses that may be in the solvent as a result of pumping.

      Back-Pressure Regulator: Balances inlet and outlet pressures going to injector.

      Injector valve: Inject sample into a loop and then move the handle and the entire loop of sample is inserted
      between the pump and the column so the mobile phase sweeps the sample onto the column.
  Column: Can be made from heavy-walled glass tubing, polymer tubing, or smooth-bore stainless steel tubing.
  There are different kinds of columns including analytical columns and guard columns.

  Detectors: The sample is then sent to some kind of detector such as a UV-Vis, Infrared Absorption,
  Fluorescence, or Refractive-Index detectors.



  Vendors:

  Agilent Technologies, Beckman Coulter INC. Cecil Instruments, Dion Corp, Perkin Elmer, Shimadzu, Theromo
  Fisher, and Varian

         The most expensive HPLC I could find was selling for $24,000. It was a USED Waters Acquity 2007
          HPLC system on
          http://www.dotmed.com/listing/740676?utm_source=base&utm_medium=search&utm_campaign=Base
         The cheapest HPLC I could find was selling for $499.00 on Ebay. It was a Walter 717 Autosampler.
          http://cgi.ebay.com/Waters-717-Plus-HPLC-
          Autosampler_W0QQitemZ110490475944QQcmdZViewItemQQptZLH_DefaultDomain_0?hash=item1
          9b9bedda8

  Articles:

  Analysis of Pharmaceuticals in Water by Isotope Dilution Liquid Chromatography/Tandem                     Mass
Spectrometry
  Brett J. Vanderford and, Shane A. Snyder
  Environmental Science & Technology 2006 40 (23), 7312-7320


  Selective Analysis of Secondary Amino Acids in Gelatin Using Pulsed Electrochemical Detection
  Jason D. Russell, John M. Dolphin, and Miles D. Koppang
  Analytical. Chemistry., 2007, 79 (17), pp 6615–6621

  Facile Identification and Quantitation of Protein Phosphorylation via β-Elimination and Michael
  Addition with Natural Abundance and Stable Isotope Labeled Thiocholine
  Meng Chen, Xiong Su, Jingyue Yang, Christopher M. Jenkins, Ari M. Cedars and Richard W. Gross
  Analytical Chemistry., 2010, 82 (1), pp 163–171


  Everyday Uses For HPLC
     1) The government uses HPLC to test confiscated narcotics
     2) It can also be used to help test environmental samples such as water from a drain area or stream.

				
DOCUMENT INFO
Shared By:
Categories:
Tags:
Stats:
views:1
posted:11/16/2012
language:English
pages:2