Hits from Viability Screen in Prostate DU145 Cells.xls by shenreng9qgrg132



Gene hits identified in a DU145 viability screen that are known to have altered expression profiles in prostate cancer
Cellecta, Inc.
Date:    9/28/2010
Library: DECIPHER Human Module 1

 Rank                                                                                             Gene Description                                                                                        RefSeq             shRNA
     1   RPL13                                                                                                                                                                                        NM_033251.1               2
                     Ribosomal protein L13; a structural constituent of ribosome that plays a role in cell cycle and positive regulation of cell growth; may act in translation; aberrant mRNA expression correlates with breast neoplasm
     2   RPL10                                                                                                                                                                                        with hepatocellular carcinomas
                     Ribosomal protein L10; a structural constituent of ribosome that inhibits phosphorylation and protein kinase activity; upregulation of the corresponding gene is associated NM_006013.2                    3
     3   NEDD8                                                                                                                                                                                        NM_006156.1               3
                     Neural precursor cell expressed developmentally downregulated 8; a transcription coactivator that acts in protein polyubiquitination and neddylation; regulates ubiquitin-protein ligase activity; decreased expre
     4   ITGA2                                                                                                                                                                                        NM_002203.3               2
                     Integrin alpha 2; a collagen receptor that acts in cell adhesion; antiapoptosis; and blood coagulation; aberrantly expressed in several cancers; gene polymorphisms are associated with risk of myocardial infarct
     5   NRAS                                                                                                                                                                                         NM_002524.2
                     Neuroblastoma RAS viral oncogene homolog; a GTPase that is involved in purine nucleoside catabolism; mitochondrion organization; and antiapoptosis; gene mutations cause malignant melanomas; myelodysp    2
     6   GPD2                                                                                                                                                                                         NM_000408.2
                     Glycerol-3-phosphate dehydrogenase 2 mitochondrial; involved in glycolysis; ROS production; and FADH2 metabolism; aberrant activity correlates with type-2 diabetes and may correlate with prostate cancer;2
     7   CDC25C                                                                                                                                                                                       NM_001790.3
                     Cell division cycle 25C; a phosphoprotein phosphatase that acts in dephosphorylation; G2 phase of mitotic cell cycle; and DNA damage checkpoint; aberrant expression correlates with breast and 2          prostate neop
     9   HSD17B3                                                                                                                                                                                      NM_000197.1               2
                     Hydroxysteroid (17-beta) dehydrogenase 3; a NADPH binding protein that converts androstenedione to testosterone in the testis; plays a role in sex determination; polymorphism in the corresponding gene cau
    10   E2F5                                                                                                                                                                                         NM_001951.3
                     E2F transcription factor 5 p130-binding; a transcriptional activator that binds to RBL2; may regulate cell cycle; mRNA is aberrantly expressed in acute myelocytic leukemia and breast and ovarian3neoplasms
    11   CD151                                                                                                                                                                                        NM_139029.1               2
                     CD151 molecule; an integrin binding protein that plays a role in cell migration; extracellular matrix organization and biogenesis; and regulation of endocytosis; increased expression of mRNA correlates with ost
    12   STEAP3                                                                                                                                                                                       NM_182915.2               2
                     STEAP family member 3; an oxidoreductase that plays a role in the induction of apoptosis and regulation of protein secretion; inhibits cell proliferation; decreased mRNA expression correlates with prostate carc
    13   PRKCD                                                                                                                                                                                        NM_006254.3               2
                     Protein kinase C delta; a phospholipase activator that mediates neutrophil apoptosis; platelet activation; lamin cleavage; intercellular junction assembly; calcium mobilization; receptor internalization; and histo
    14   DKK3                                                                                                                                                                                         NM_013253.4
                     Dickkopf homolog 3; a thyroxine 5'-deiodinase that acts in thyroid hormone generation; respiratory gaseous exchange; locomotion; and induction of apoptosis; mRNA is downregulated in schizophrenia; non-sm2
    16   RPS6KB1                                                                                                                                                                                      NM_003161.2
                     Ribosomal protein S6 kinase 70kDa polypeptide 1; acts in GPCR pathway; regulates protein synthesis; cell cycle; and apoptosis; protein expression is increased in tuberous sclerosis and breast cancers    2
    17   TNFAIP3                                                                                                                                                                                      NM_006290.2               2
                     Tumor necrosis factor alpha-induced protein 3; acts in antiapoptosis and protein deubiquitination; decreased expression correlates with Crohn disease; gene polymorphisms are associated with increased risk of
    18   NES                                                                                                                                                                                          brain abscess;
                     Nestin; an intermediate filament that functions in cell migration and inflammatory response; may act in nervous system and skeletal muscle development; upregulated in NM_006617.1 epilepsy;2glioma; Creut
    19   RXRA                                                                                                                                                                                         NM_002957.3               3
                     Retinoid X receptor alpha; a DNA binding protein that regulates apoptosis; transcription; and cell growth; involved in monocyte differentiation; aberrant protein expression is associated with several neoplasms;
    20   MCM2                                                                                                                                                                                         NM_004526.2               3
                     Mini chromosome maintenance deficient 2; binds to kinases; acts in cell growth; mitotic cell cycle checkpoint; and regulation of chromosome organization; aberrant expression is associated with oral epithelial d
    21   HSD3B2                                                                                                                                                                                       NM_000198.2               2
                     Hydroxy-delta-5-steroid dehydrogenase 3 beta- and steroid delta-isomerase 2; a putative steroid dehydrogenase that may play a role in steroid biosynthetic process; downregulated in adrenal hyperplasia; gen
    22   NTRK1                                                                                                                                                                                        NM_001007792.1            2
                     Neurotrophic tyrosine kinase receptor type 1; regulates apoptosis; aberrantly expressed in Alzheimer disease and several neoplasms; mutations cause congenital insensitivity to pain with anhidrosis and heredi
    23   CHGB                                                                                                                                                                                         NM_001819.1
                     Chromogranin B; a cytoplasmic membrane bound vescicular protein; upregulation correlates with adenocarcinoma; gastrinoma; and multiple neoplasms; decreased expression correlates with Alzheimer disease   2
    24   FST                                                                                                                                                                                          NM_006350.2               2
                     Follistatin; a transcriptional regulator that acts in cell proliferation; female pregnancy; and utero embryonic development; expression is upregulated in liver diseases and sepsis; aberrant mRNA expression corr
    25   TERT                                                                                                                                                                                         NM_198253.2
                     Telomerase reverse transcriptase; an RNA-directed DNA polymerase that functions in telomere maintenance; aging; DNA repair; and antiapoptosis; abnormal gene expression correlates with melanoma; neuro    2
    26   TOP1                                                                                                                                                                                         NM_003286.2
                     Topoisomerase I; an endonuclease that plays a role in DNA ligation and recombination; induces DNA synthesis and cell proliferation; upregulated in colorectal neoplasms and non-Hodgkin lymphoma; gene tran2
    27   CYP1A1                                                                                                                                                                                       NM_000499.2
                     Cytochrome P450 family 1 subfamily A polypeptide 1; a monooxygenase that plays a role in metabolism of steroids; retinoids; and xenobiotics; upregulated in breast neoplasms; gene polymorphism correlates 2
    28   PERP                                                                                                                                                                                           of heterozygosity is associated with n
                     TP53 apoptosis effector (keratinocytes associated protein 1); mRNA expression is altered following p53-induced apoptosis in leukemia cells; decreased expression and lossNM_022121.2                       2
    29   DAXX                                                                                                                                                                                         NM_001350.3
                     Death-associated protein 6; an apoptotic protease activator that acts in apoptosis and chromatin remodeling; upregulated in Alzheimer disease and several neoplasms; aberrant mRNA expression2is associated
    30   CAV1                                                                                                                                                                                         NM_001753.3               2
                     Caveolin 1; regulates apoptosis; aberrant expression correlates with various cancers; neuro diseases; arteriosclerosis; gene mutation correlates with hypotension and metabolic disorder; mouse Cav1 correlates
    31   PCNA                                                                                                                                                                                          asthma; psoriasis; arteriosclerosis; an
                     Proliferating cell nuclear antigen; a transcriptional regulator that acts in cell proliferation and DNA replication and repair; aberrantly expressed in glioblastoma; melanoma;NM_002592.2                 2
    32   AHR                                                                                                                                                                                          NM_001621.2               2
                     Aryl hydrocarbon receptor; a transcription regulator that acts in cell cycle; apoptosis; blood vessel patterning; circadian behavior; and inflammatory response; protects from bacterial infections; upregulated in
    33   TSG101                                                                                                                                                                                       NM_006292.2
                     Tumor susceptibility gene 101; a transcriptional regulator that binds and stabilizes NR3C1; mediates multivesicular body formation in endosomes; involved in endocytic sphingomyelin trafficking; 2        abnormal exp
    34   MIF                                                                                                                                                                                          NM_002415.1               2
                     Macrophage migration inhibitory factor; a cytokine that regulates apoptosis; aberrant expression is associated with arteriosclerosis; dermatitis; Guillain-Barre syndrome; lupus erythematosus; sclerosis; myoca
    35   EIF4EBP1                                                                                                                                                                                     NM_004095.3               3
                     Eukaryotic translation initiation factor 4E binding protein 1; a translation factor that acts in insulin receptor signaling pathway; regulates lipid metabolism and protein kinase B signaling cascade; upregulated in
    36   SKP2                                                                                                                                                                                          catabolic process; upregulated in vario
                     S-phase kinase-associated protein 2; an ubiquitin-protein ligase that plays a role in G1-S transition of mitotic cell cycle; cell proliferation; and ubiquitin-dependent protein NM_005983.2               2
    37   CXCL12                                                                                                                                                                                       NM_000609.4               3
                     Stromal cell-derived factor 1a; a CXCR4 chemokine receptor binding protein that mediates angiogenesis; antiapoptosis; cell adhesion; and platelet formation; aberrantly expressed in several neoplasms; gene p
    38   KRAS                                                                                                                                                                                         NM_033360.2
                     Homolog of v-Ki-ras2 Kirsten rat sarcoma viral oncogene; a GTPase that acts in activation of MAPK activity and DNA damage response signal transduction resulting in induction of apoptosis; gene2           mutation is as
    39   CALR                                                                                                                                                                                         NM_004343.2               2
                     Calreticulin; a Ca2+ binding chaperone that acts in N-glycan processing; neutrophil activation; protein folding; and lactation; upregulated in pre-eclampsia; goiter; and several cancers; autoantibodies are asso
    40   EDNRA                                                                                                                                                                                          mRNA expression correlates with auto
                     Endothelin receptor type A; a GPCR that induces cell proliferation; increased expression correlates with dilated cardiomyopathy; breast and colorectal neoplasms; aberrantNM_001957.1                      2
    41   RAD21                                                                                                                                                                                        in Down syndrome; aberrant mRNA ex
                     RAD21 homolog; a sequence-specific DNA binding protein that acts in attachment of spindle microtubules to kinetochore and cell cycle arrest; protein level is upregulated NM_006265.1                      3
    42   NCOA3                                                                                                                                                                                        NM_181659.1
                     Nuclear receptor coactivator 3; a histone acetyltransferase that induces cell proliferation; triggers JNK (MAPK8) cascade; upregulation of the corresponding gene is associated with various cancers       2
    43   CFLAR                                                                                                                                                                                        NM_003879.3
                     CASP8 and FADD-like apoptosis regulator; a caspase inhibitor that acts in MAPKKK and NF-kappaB cascade and antiapoptosis; downregulated in Alzheimer disease and chronic B-Cell lymphocytic 2              leukemia; upr
    44   RUNX2                                                                                                                                                                                        NM_004348.3
                     Runt-related transcription factor 2; acts in odontogenesis; upregulated in arteriosclerosis and calcinosis; gene mutation causes cleidocranial dysplasia; mouse Runx2 is associated with dysplasia; 2      osteopenia; an
    45   HSD17B2                                                                                                                                                                                      NM_002153.1               2
                     Hydroxysteroid 17 beta dehydrogenase 2; plays a role in 17-beta estradiol metabolism; downregulated in endometriosis and prostate cancer; aberrant expression of the corresponding gene is associated with c
    46   PDPK1                                                                                                                                                                                        NM_031268.4
                     3-phosphoinositide-dependent protein kinase-1; a cAMP-dependent protein kinase regulator that regulates apoptosis; transforming growth factor beta receptor signaling pathway; and leukocyte chemotaxis; pr2
    47   PTPN1                                                                                                                                                                                        NM_002827.2               2
                     Protein tyrosine phosphatase non-receptor type I; acts in protein dephosphorylation and glucose homeostasis; regulates apoptosis and protein kinase activity; upregulated in breast neoplasms; gene polymorph
    48   CTGF                                                                                                                                                                                         NM_001901.2               2
                     Connective tissue growth factor; mediates ossification; angiogenesis; and cell proliferation; aberrant expression is associated with keloid; muscular dystrophy; diabetic nephropathy; Alzheimer disease; cerebra

Cellecta, Inc., DECIPHER shRNA Library Screen Data                                                           9/28/10                                                                                                       Page 1 of 2

 Rank                                                                                            Gene Description                                                                                       RefSeq          shRNA
    49   CD24                                                                                                                                                                                       neoplasms; gene polymorphism is ass
                    CD24 molecule; a signal transducer that acts in cell adhesion and induction of apoptosis; protein expression is upregulated in intrahepatic cholangiocarcinoma and several NM_013230.2                  2
    50   TLR1                                                                                                                                                                                       NM_006068.2             2
                    Toll-like receptor 1; forms a heterodimer with TLR2; regulates macrophage activation; protein expression is upregulated in arteriosclerosis and tuberculosis; gene polymorphisms correlate with aspergillosis; as
    51   AREG                                                                                                                                                                                       asthma; multiple myeloma; HIV infect
                    Amphiregulin; an EGFR activating ligand that acts in antiapoptosis; chemotaxis; cell proliferation; prostaglandin biosynthesis; and wound healing; aberrantly expressed in NM_001657.2                  2
    52   TGFBR2                                                                                                                                                                                     NM_001024847.1          2
                    Transforming growth factor beta receptor II; interacts with Itgb3; acts in aging and regulation of cell proliferation; increased expression correlates with arteriosclerosis; gene mutation is associated with T cell ly

Cellecta, Inc., DECIPHER shRNA Library Screen Data                                                          9/28/10                                                                                                      Page 2 of 2

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