Pharmacokinetic study of chitosan based Methotrexate micro particles.
Patel H. V.1, Patel P. J.2, Patel B.G.3, Patel V.A.4
1, 2: Indukaka Ipcowala College of Pharmacy, New V. V. Nagar-388121, Gujarat, India.
3: Charusat, Changa, Petlad, Anand- 388 421, Gujarat, India.
4: A.R College of pharmacy, Vallabh vidhya nagar-388120, Gujarat, India.
The present work was aimed to evaluate the pharmacokinetic behavior of the micro particles of
Methotrexate. Chitosan based Methotrexate micro particles prepared by emulsion crosslinking
method. A study of the pharmacokinetics and bio distribution of the formulation in suitable animal
model during the later stages of the development is important for attaining the desired product
performance. For any new formulation, there should be in-vitro in-vivo correlation. The in-vitro
study should be reproducible and mimic the in-vivo behavior. The in-vitro profile can be useful for
forecasting its in-vivo effect. But one cannot confirm the performance without in-vivo
investigation. The relationship should be established only after its pharmacokinetic and bio
distribution analysis. Wistar healthy female albino rats weighing between 225-250 g were selected
for the study. Rats were fasted overnight but water was provided. After appropriate dilutions of the
stock suspension of micro particles were immediately made before injection. It was injected
intramuscularly into the gastrocnemius muscle of the right hind leg by 1mL syringe with 18 gauge
needle. At predetermined time intervals, blood was collected in a heparinized centrifuge tube from
the retro-orbital plexus with aid of capillary tube and analysis of Blood and Tissue samples. The
results of in-vivo study were analyzed with the methods of first order elimination. The
pharmacokinetic parameters were generated by non compartmental mathematical expression from