Use of the New LACF 590 Commonwealth Ave. • The new facility is split into a Barrier and Non Barrier Facility • Both are considered “clean” of rodent pathogens including noro- virus. Barrier vs Non-Barrier • The Barrier is used for the breeding of mouse lines that are not commercially available. • Once a mouse leaves the Barrier it does not come back. • The Non-Barrier is used once a mouse is involved in an experiment, surgery, behavioral testing, etc. • Mice in the Non-Barrier can leave for surgery, behavioral testing, etc. and return. Non-Barrier • Besides experimental mice, the Non- Barrier also houses rats, ferrets, gerbils, zebra finch birds, and both mouse and rats for ABSL-2. • In the Non-Barrier there is also a necropsy room, a surgical suite, cagewash facilities and autoclave facilities. Necropsy • Necropsy is to be used only for terminal procedures. • Neither animals nor people can return to an animal room after being in the Necropsy. Surgical Suite • The Surgical Suite consists of 3 rooms. – People and instrument prep room. – Actual surgical room. – Animal prep room, which is also the post op recovery room. – This animal prep/post op recovery room can also be used as a procedures room. Clean and Dirty Caging • There will be a cart outside the cagewash room for the placement of dirty cages. • There will be a limited but sufficient amount of clean cages, bottles, tops etc available in each rodent room along with cagecards, colored dots and other materials. Traffic Flow • Once again flow is from the cleanest to the dirtiest. • Start with the Barrier Facility which is of limited access. • Then the clean side of the Cagewash. • Then the Mouse room and the rat room • Then the bird room & then the ferret room. • The last two rooms to be entered are the ABSL- 2 room, and the gerbil room • And finally the dirty side of the cagewash. • PLEASE do not go from the dirtier to the cleaner areas. • Once you have been in the Non-Barrier area you CAN NOT go back into the Barrier. • Once you have been in the dirty side of the cagewash you should not go back into the animal rooms. • Once you have been in Necropsy you can not go into another animal room. PPE • For Entry into hallways – Disposable lab coat – Disposable bonnet – Disposable booties – Disposable gloves – Disposable masks • For Entry into animal rooms & handling of animals or animal cages. All of the above, PLUS Disposable arm covers. Gowning • Leave lab coats, jackets etc in the entry area. • Put on lab coat, bonnet and mask. • Put on booties as you cross the imaginary line into the LACF. • Put on gloves after you put on booties. THANK YOU!!!!
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