AP Biology Campbell 8th Edition Chapter 20 Study Guide Due_____ On an attached sheet of paper, describe the following concepts. Connect as many as possible (to each other), use diagrams where appropriate. 1. bacterial artificial 18. genetically modified 33. restriction fragment length 2. chromosome (BAC) (GM) organism polymorphisms (RFLPs) 3. biofuel 19. genomic library 34. restriction site 4. biotechnology 20. Huntington’s disease 35. reverse transcriptase 5. cDNA library 21. in situ hybridization 36. polymerase chain reaction 6. clone 22. in vitro mutagenesis (RT-PCR) 7. cloning vector 23. northern blotting 37. RNA interference (RNAi) 8. complementary DNA 24. nucleic acid 38. short tandem repeats (STRs) (cDNA) 25. hybridization 39. sickle-cell disease 9. DNA ligase 26. nucleic acid probe 40. single nucleotide 10. DNA microarray assay 27. Parkinson’s disease polymorphisms (SNPs) 11. electroporation 28. plasmid 41. Southern blotting 12. expression vector 29. pluripotent 42. stem cell 13. gel electrophoresis 30. polymerase chain 43. sticky end 14. gene cloning reaction (PCR) 44. Ti plasmid 15. gene therapy 31. recombinant DNA 45. yeast artificial chromosome 16. genetic engineering 32. restriction fragment (YAC) 17. genetic profile Genetic Engineering Poster: Research a genetically engineered product or organisms of your choosing. Compare side-by-side the genetically engineered item to the conventional (non transgenic) methods of fulfilling that same need in a poster presentation. For example you can compare the use of pig insulin for diabetics to the use of human insulin gene inserted into bacteria, or traditional hepatitis vaccine to genetically engineered crops with hepatitis vaccine. Include in your poster the following: Conventional/Traditional Method Genetically Engineered Method /5pts /10pts Describe in detail how we used to fill Describe how your chosen product is genetically engineered (be our need prior to genetic engineering. specific in the biotechnological process, including scientific names, restriction enzymes, vectors, where the gene came from and what it was put into, the protein for which the gene codes and what it does) /5pts /5pts Pictures and diagrams supporting the Pictures and diagrams supporting the current biotechnology of your conventional production of your chosen product. chosen product /4pts /4pts The benefits of your chosen product The benefits of your chosen product being genetically engineered. being conventionally produced /4pts /4pts The drawbacks of your chosen product The drawbacks of your chosen product being genetically engineered. being conventionally produced /1.5pts /1.5pts Bibliography Bibliography Be prepared to discuss your opinion of how society has been affected by the biotechnology of genetic engineering. 40pts You may work with 1 partner, you must clear topics with me, no duplication of topics Due to Murphy’s Law, (and in order to avert technical difficulties, computer glitches, time warps, black holes, gremlins, natural and unnatural disasters) please do not wait until the last minute to complete or print your project. Late projects are assessed 10% per day.
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