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					LISTERIOSIS IN CHINCHILLAS (Chinchilla laniger) - BIOLOGICAL EXPERIMENT

Alenka Dovc*, Irena Zdovc**. Nastja Jaglicic-Korpic***, Veterinary Faculty, Institute for Health Care of
Poultry, Cesta v Mestni log 47, 1000 Ljubljana Slovenia'1*, Veterinary Faculty, Institute for microbiology
and parasitology, Gerbiceva 60, 1000 Ljubljana, Slovenia**, Veterinaria d, oo „ Lendavska 27, 9000 Murska
Sobota, Slovenia***

Problem
Listeriosis is among the most frequent diseases in chinchillas (Chinchilla Ianiger), since this species of
animals is more susceptible to Listeria monocytogenes when compared to other species. Clinically: varoius
forms are known: depression and loss of appetite, diarrhoea, torticollis and often leads to fatal forms of
encephalitis.

Method
During two years period infection with Listeria monocytogenes was confirmed in 55, 6% of examined breed
of chinchillas (Chinchilla laniger) in Slovenia. The illness in Chinchillas causes serious healthy problems
and economic loss. In intention to followed the transmission and the manner of spreading of infections, we
made a biological experiment with white laboratory mice and chinchillas. For the inoculation two strains
were used; one originally isolated from chinchillas and one isolated from a cattle. Inoculate was prepared in
TSB and contains 108 listerias in 1 ml. Animals were inoculated p/o (direct application in stomach) and i/p
with a, different doses of listerias. After inoculation immunological response was monitored by complement
fixation test, enzyme immuno assay and gel precipitation teat. Organs (brain, liver, spleen, kidney and uterus)
were delivered for the bacteriological examination.

Results and conclusion
Listeria monocytogenes was reisolated from brain, liver", spleen, kidney and uterus from orally and
parenterally inoculated animals. The results showed that for the biological experiments much higher
concentration of contagious materials should be used for oral inoculation (30, 0 µl) compared to parenteral
inoculation (1, 0 µl). In orally inoculated animals listerias were isolated after 10 days and latter, in
parenterally inoculated animals first positive results were obtained within two days after challenge,
Immunological reactions have not given satisfying results both in chinchillas and white laboratory mice,
LISTERIOSIS IN DENMARK 1998- 2000 - CLINICAL AND EPIDEMIOLOGICAL
ASPECTS

P. Gerner-Smidt, B. Bruun, V. Fussing, J. Engberg, A.M. Petersen, J. Schiellerup, Statens
Serum Institut, Artillerivej 5, Copenhagen, Denmark

Denmark has a high registered incidence of listeriosis of 0.7- 0.8/100, 000 inhabitants/
year.
The disease is statutory notifiable through a laboratory notification system. On a voluntary
basis, the strains and detailed clinical information are collected by the national reference
centre at Statens Serum Institut. The strains are routinely O-grouped and ribotyped using
the automated Riboprinter system. Strains not discriminated are further compared by
PFGE. One-hundred-twenty-four cases were notified during 1998- 2000. Twenty-five
deaths were registered. Two were abortions, 3 newborns, and 20 above 46 years of age.
There were no increase in the death rate with increasing age above 50 years. Seventy-
one patients had predisposing conditions with the most common being haematologic
malignancy, other cancer, immunosuppressive treatment, and chronic alcoholism. The
case-fatality rate was the thrice as big in patients with a predisposing condition compared
to previously healthy patients (25% ~ 8.2 %). The clinical presentation of the cases were
13 maternofetal, 83 septicaemia, 23 meningitis, and 5 other. The mortality was for
newborns 27%, for septicaemia 21%, and for meningitis 14%. Sixty-four per cent of the
strains were serogroup 1/2. No clusters of cases infected with the same type were
observed. Listeriosis remain a serious disease in Denmark where we seem to have a
hyper-endemic situation compared with other industrialised countries.

				
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