Microscope by HC121104002213

VIEWS: 0 PAGES: 15

									   Clinical Microscopy


“The Microscope”
• Halogen Bulb
• Usually
  controlled by a
  rheostat (knob
  that controls
  the intensity).

           Illuminator
• Compound lens system that focuses the light
  from the illuminator onto the specimen.
• Gathers a large amount of light and condenses
  it into a small area.




  Condenser
• Lever on the front of the condenser that
  allows you to control the amount of light
  leaving the condenser.
• Opens and closes like the iris of you eye.




   Aperture Diaphragm
• Simply the place where the slide sits.
• This area becomes very dirty. Keep it clean.




        Stage
• Mechanical device that holds the slide in
  place on the stage. Where ever the stage
  goes the slide goes!




   Slide Holder
• Traditionally come in
  four flavors in the lab
  – 10x, 40x, 50x, and
    100x
• Magnify the image

                            •50x and 100x require
                            immersion oil.
                               • The oil bends light
Objectives                     waves so more light
                               enters the objective
• Usually have a magnification power of 10x.
• Form the final image.
• Total Magnification: equals the oculars
  time the objectives. (40x times 10x = 400)


   Oculars
• Two flavors of these:
  – Course
  – Fine
• Some microscopes control the course and
  fine focus with one knob.




                          Focus Knobs
• Controls the stage movement.
• Top knob controls forward and back movement.
• Bottom knob controls left and right movement.




                   Stage Adjustment
                   Knobs
               Cleaning the Microscope
• Keeping the microscope
  clean is extremely
                                4
  important.
• Use a clean, lint-free lens
  tissue.                           3
                                         5
• Don’t use a dry tissue to          2
  clean the lenses. Use a           1
  moist tissue first and then
  a dry one to mop up.
• Start by cleaning the
  illuminator.                Cleaning...
• Move to the
  condenser
                          4
• Next clean the
  objectives, but clean
  the 100x last               3
                                     5
  because there may            2
  be oil on it. Once          1
  you have cleaned the
  100x, get a new
  tissue.
                             Cleaning...
• Clean the oculars
• Lastly, wipe off the
  stage. Clean it last   4
  because it usually
  has the most dust
                             3
  and oil.                          5
• Make this a daily           2
  routine.                   1

								
To top