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                                    ÉCOLE DOCTORALE 145
                      « SCIENCES DU VÉGÉTAL DU GENE A L’ECOSYSTEME »



    THESES SUBJECTS FOR COMPETITION 2012
TITLE
Analysing the action of non-coding RNA in root developmental plasticity

SUPERVISOR:
Martin Crespi                        email: martin.crespi@isv.cnrs-gif.fr


AFFILIATION
Institut des Sciences du Végétal (Dir. Hélène Barbier-Brygoo), CNRS, Gif sur Yvette


SUMMARY

Non-protein coding RNAs (or npcRNAs) can be (catalytic or structural) components of
ribonucleoprotein complexes (RNPs) and/or precursors of small RNAs such as the si/miRNA.
They may control mRNA stability or translation of specific targets in development and abiotic
stress responses or can be involved in different epigenetic processes. We have recently
identified 76 npcRNAs from Arabidopsis from which 3 are novel precursors of siRNAs and
are regulated by auxin or cytokinins during lateral root formation.
This project aims to decipher the mechanisms of action of these 3 RNA regulators in root
developmental plasticity using reverse genetics and cell biology approaches. Using
transcriptional fusions and RNA-tagging approaches in vivo, we will follow the eventual
interactions of these npcRNAs with different cellular RNPs (e.g; P-bodies, polysomes and
splicing speckles). Using mutants affected in si/miRNA biogenesis (DCLs, RDRs,AGOs), we
will characterise their biosynthesis. The interaction of npcRNAs with known or new RNPs
will be followed using confocal and electron microscopy to add a subcellular dimension to
this analysis. This project will allow us to integrate riboregulatory mechanisms in the
adaptation of root architecture to the environment.

Key Words
Non protein coding RNAs, small RNAs, in vivo RNP localisation, root differentiation, lateral
root formation, RNA-protein interactions, epigenetics




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