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							NATIONAL HEALTH AND MEDICAL RESEARCH COUNCIL

Final Report on the Use of Excess ART Embryos ________________________________________________________
Licence Number: (insert licence number) Licence Holder: (insert licence holder as detailed on the licence) Activity Title: (insert activity title as detailed on the licence) Licence Issue Date: (insert licence issue date) Licence Expiry Date: (insert licence expiry date) ___________________________________________________________________

1.

Excess ART embryos consented for use in the licensed activity When completing the attached spreadsheet please include every excess ART embryo for which proper consent was obtained to use the embryo in the authorised activity (one line per embryo). If the Licensing Committee has previously given approval to use an alternative spreadsheet format for the 6monthly reports specified by Standard Licence Condition 3001, that spreadsheet may be used for this report also.

2.

Aims of the licence Provide a report of the activity undertaken and outcomes achieved during the period of the licence. Please include information on - The number of excess ART embryos used - The number of excess ART embryos remaining for which consent was obtained and the procedures for dealing with them. - How the licensed use of excess ART embryos has achieved “a significant advance in knowledge or improvement in technologies for treatment.” - If your licence involved the derivation of stem cell lines please complete question 3. If not, please go to question 4.

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3. -

Establishment of Human Embryonic Stem Cell Lines How many hESC lines have been confirmed as established (include cell line identifiers)? How many putative hESC lines are under evaluation (include cell line identifiers)?

Please use the table below to provide information about each of the cell lines listed above. Use one column for each established or putative cell line. Copy the table as necessary to accommodate all cell lines. Information requested by the Licensing Committee 1. hESC line name or identifier 2. Embryo identification number 3. hESC line status – confirmed/ under evaluation/discarded 4. Karyotype 5. Which hESC surface marker proteins were detected? 6. What gene expression studies have been performed? 7. How have you demonstrated the pluripotency of the cell line? 8. Number of passages 9. How many times have the cells been successfully cryopreserved? 10. What tests for microbiological contaminants have been conducted? 11. What species were the feeder cells derived from? 12. Were animal products used in the derivation of the cell line? Established or Putative Human Embryonic Stem Cell (hESC) Lines

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13. Who has the cell line been supplied to? 14. Do you have any other relevant information that you think would interest the Licensing Committee?

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4.

Procedures for ensuring that the licensed activity will cease when the licence expires

Describe the protocols for ensuring that the licensed activity ceased when the licence expired. Describe the protocols for ensuring that no further excess ART embryos will be thawed for the activity following expiry of the licence. Describe the fate of any unused excess ART embryos for which consent was obtained.

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