Utilizing Peanut (Arachis hypogaea) extract as a potential source by GoPkbJ

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									UTILIZING PEANUT (ARACHIS HYPOGAEA)
    EXTRACT AS A POTENTIAL SOURCE OF
  RESVERATROL IN ACTIVATING SIRT1 GENE
                          CHAPTER 1
                    PROBLEM AND ITS SETTING
     INTRODUCTION


•   In the current condition of our state today, cancer is one of the world’s most feared
    and fearful diseases of all diseases that affect humanity. Cancer is fast becoming an
    epidemic in the new world and science is still unable to explain its complexity and get
    a suitable cure. The disease is one of the ills that as happened to humanity.
•   SIRT 1 is a gene found in humans and other mammals that helps to promote survival
    by protecting cells during times when food is scarce. It acts as a “rescue gene”
    repairing the damage done by free radicals and preventing cells from dying
    prematurely. The gene also causes mitochondria, the power plants of cells, to produce
    energy at higher levels that are typically associated with younger cells. As a result,
    SIRT 1 gene is believed to be a principal regulator of lifespan.
•   SIRT 1 gene will not be useful without the presence of resveratrol. Resveratrol is the
    plant nutrient that is getting a great ideal for media attention. SIRT 1 is activated by
    calorie restriction or by the compound in red wine known as resveratol. It makes
    healthy cells less sensitive to radiation and it can be used to modulate the growth of
    cancer cells. Resveratrol has the effect of preventing damage of cells before they
    develop into a state that triggers cancer. In short, resveratrol helps to kill cancer cells.
•   In the field of study on utilizing Peanut (Arachis hypogaea) extract into processing a
    resveratrol was carefully studied to maneuver it to be possible.
STATEMENT OF THE PROBLEM
•    This study ill determine the effectiveness of Peanut (arachis hypogaea)
    extract as a potential source of Resveratrol in activating SIRT 1 a gene to fight
    cancer. Specifically it aims to answer the following questions:




•         Do the peanut(Arachis hypogaea) extract has the capacity
    of being o potential source of resveartrol?
•        What is the resveratrol percentage recovery from peanut
    (Arachis hypogaea) extract?
OBJECTIVES OF THE STUDY
• This study aims to:



  • Determine the potentiality of
    peanut(Arachis hypogaea) extract as a
    source of resveratrol.
  • Determine the percentage recovery of
    the peanut(Arachis hypogaea) extract.
          HYPOTHESES

• Peanut(Arachis hypogaea) extract is not a
  potential source of resveratrol.
• Peanut(Arachis hypogaea) extract is
  insufficient to reach the satisfactory
  percentage recovery to be a potential
  source of resveratrol.
SIGNIFICANCE OF THE STUDY

• Generally, this study is an important endeavor that will determine that
  peanut(arachis hypogaea) extract is now the new potential source of
  resveratrol.
• There may be lots of research studies known today to produce
  resveratrol, however, because of its reported lack of supplies; majority
  of this products are not available in the market today.
• The product of this study may furnish a far cost-effective resveratrol
  product against the inadequate amount of the generation’s sources.
• Since the availability of peanut extract in the world is high, this
  research proposal will make way for a cheaper price of resveratrol
  today.
CONCEPTUAL FRAMEWORK
       Peanut (Arachi hypogaea)
                 extract

      Extraction of resveratrol
      from Peanut
      (Arachi hypogaea)

Activating SIRT 1 gene through resveratrol


 Potential source in killing cancer cells
        SCOPE AND DELIMITATION

•   The study entitled “Utilizing Peanut (arachis
    hypogaea) extract as a potential source of
    resveratrol on activating SIRT 1 gene “ focuses
    on the peanut (arachis hypogaea) extract on
    helping produce new source of resveratrol. The
    study is only limited in peanut (arachis
    hypogaea).
                                   RELATED LITERATURE

                                  Peanut- is a species in the legume. The cultivated peanut was
                                  probably first domestricated in the valleys of Peru. It is an annual
                                  herbaceous plant growing 30 to 50 cm tall. The leaves are
                                  popposite, pinnate with four leaflets, each leaflet 1 to 7 cm long
                                  and 1 to 3 cm broad. The flowers are a typical peaflower. In
                                  shape, 2 to 4 cm across, yellow with reddish veining. After
                                  pollination, the fruit develops into a legume 3 to 7 cm long,
     Peanut (Arachis hypogea)
                                  containing 1 to 4 seeds, which forces its way underground a
     Scientific classification    mature. Hypogaea means “under the earth”. Peanut has only
Kingdom:            Plantae       occasionally been grown in Northern States due to its warm
(unranked):         Angiosperms
(unranked):         Eudicots
                                  temperature requirement. Use of poorly adapted varieties and
(unranked):         Rosids        improper production practices usually resulted in low yields and
Order:              Fabales       poor quality nuts. However, peanut could be an alternative cash
Family:             Fabaceae
Subfamily:          Faboideae     crop to soybean, corn, potato or fieldbean.
                    Aeschynomen
Tribe:
                    eae
Genus:              Arachis
Species:            A. hypogaea
         Binomial name
        Arachis hypogaea
                L.
•   Resveratrol- essentially, the resveratrol compound is classified in a pamily that’s known
    as polyphenols. In small amounts, resveratrol can be found naturally in grapes, some
    green teas and even peanuts. It is also the main ingredient that gives red wine natural
    anti-aging propertie.
•   SIRT 1 gene- or “skinny gene” assists with weight loss by inhibiting food storage and
    increasing fat metabolism. By causing the body to store fewer fat cells, SIRT 1 can also
    slow the aging process by reducing t5he risk of age- related diseases and health threats,
    including heart attack and stroke, diabetes, arthritis and osteoporosis. These conditions
    have all been linked to an excess amount of fat cells in the sky.
•   Cancer- is the general name for a more than 100 diseases in which cells in a part of the
    body begin to grow out of control. Although there are many kinds of cancer, they can start
    because abnormal cells grow out of control. Untreated cancers can cause serious illness
    and even death.
•
                      CHAPTER II
                     METHODOLOGY
       MATERIALS/EQUIPMENT

        For the extraction of resveratrol from the peanut
(Arachis hypogaea) extract the following materials that will be
used: Rotavap for extracting the peanut, ethanol for
separating resveratrol from the peanut (arachis hypogaea)
extract, large metal containers (extractors) where the plant
source will be placed, peanut, laboratory test equipment for
plants, column of silica, vacuum dryer to remove alcohol.
    RESEARCH DESIGN
•                  The experimentation will be done in five phases as
     follows: 1.) Collecting of peanut (arachis hypogaea) samples. 2.)
     Extracting of peanut (arachis hypogaea) 3.) Separating
     resveratrol extract from the peanut extract. 4.) Activating SIRT1
     gene through resveratrol. 5.) Potantial source in killing cancer
     cells.
    PROCEDURES

•    Collection of Peanut (arachis hypogaea) Samples
•                          The peanut (arachis hypogaea) samples will be collected and will be
     put in a container.
•           2.3.2 Separating/Getting Resveratrol Extract
•                        In order to get resveratrol extract, peanut (arachis hypogaea) is tested
     to ensure that it is not contaminated by chemicals and other materials. Once it has been
     cleared, the plant source is placed in large metal containers called extractors. Alcohol
     such as ethanol and methanol are used to separate the resveratrol extract from the
     peanut extract. The liquid that is produced is then processed even further through various
     procedures such as exposure to high pressure. Once it has been purified, the resveratrol
     extract is then vacuum-dried to remove the alcohol.
• APPLICATION
•         The extracted resveratrol will be applied to the cancer cells.

•   ANALYZING THE RESULTS
•          The cancer cells behavior will be analyzed upon how they interact with the
    resveratrol.
FLOW CHART

  Collection of      Analysis of the
  Peanut Sample      Results


  Extraction of      Results
  Peanut


  Separating
  Resveratrol        Treatment to
  Extract            Cancer cells


  Activating SIRT1   Laboratory test
  gene
PREPARED BY:

 JOUE L. VALLENTE
Thank you!

								
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