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					         OIE Reference Laboratory for Salmonellosis
                                   Annual Report for 2010

   Name of disease (or topic) for
 which you are a designated OIE                                                    Salmonellosis
         Reference Laboratory:

                                                                      Federal Institute for Risk Assessment (BfR)
                             Address of laboratory:                                 P.O.Box 330013
                                                                             D 14191 Berlin, GERMANY


                                                 Tel.:                             (+49-30) 84.12.22.12


                                                 Fax:                              (+49-30) 84.12.29.52


                                     e-mail address:                           matthias.hartung@bfr.bund.de


                                             website:                             http://www.bfr.bund.de


                                     President:                            Prof. Dr Dr A. Hensel

             Head of the Department                                             Prof. Dr B. Appel
                  ‘Biological Safety’

     Name of Head of Laboratory                                                Dr Reiner Helmuth
          (Responsible Official):

Name of OIE Reference Expert:                                                     Dr M. Hartung

      Name of writer of this report
         (if different from above):




Annual reports of OIE Reference Laboratories and Collaborating Centres, 2008                                        1
                                                     Salmonellosis




                   Summary of general activities related to the disease

The OIE Reference Laboratory is part of the BfR-unit 'Epidemiology and Zoonoses'. The unit is responsible for the
national monitoring on salmonella and other zoonotic agents and the collection of data reported by the Federal 'Länder'.
The evaluated data are used to prepare the German report on trends and sources of zoonoses and zoonotic agents in
foodstuffs, animals and feedingstuffs including information on foodborne outbreaks and antimicrobial resistance in
zoonotic agents according to the EU-Zoonoses directive (2003/99/EC).

1.   Test(s) in use/or available for the specified disease at your laboratory

     The National Veterinary Reference Laboratory for Salmonella (NRL-Salm) types Salmonella isolations from
     animals, food, feed and the environment. The isolates are received from governmental institutions, universities,
     private companies, veterinarians etc. from all 16 Federal 'Länder' in Germany.


                  Test                            For                         Specificity                     Total

            Slide agglutination           Antigens Salmonella                   Serotype                      4894

              Phage typing                   S. Typhimurium                    Phage type                     1570
                                               S. Enteritidis                  Phage type                      421
                                                S. Virchow                     Phage type                       0
                                                 S. Hadar                      Phage type                       0
                                         S. Paratyphi B dTart. +               Phage type                      107

       Antimicrobial susceptibility     Resistance (Salmonella)            Resistance pattern                 3922
                testing                   Resistance (E. coli)             Resistance pattern                 1958
                                         Resistance (MRSA)                 Resistance pattern                 1882

              PCR detection                    Salmonella                         Genus                         59

                   PCR                     Salmonella enterica           d-tartrate fermentation               114

             Plasmid Profiling                 Salmonella                    Plasmid pattern                    0

                  PFGE                         Salmonella                     PFGE pattern                      17

               Ribotyping                      Salmonella                     Ribo pattern                      0

                 MLVA                   Enteritidis/Typhimurium                 Allel no.                       32

        Resistance Determinants                Salmonella                                                      154



2.   Production and distribution of diagnostic reagents

     The BfR produces diagnostic reagents for biochemical diagnosis and differentiation of Salmonella spp. They were
     used in our own laboratory only.


                         Activities specifically related to the mandate
                                 of OIE Reference Laboratories

3.   International harmonisation and standardisation of methods for diagnostic testing or the
     production and testing of vaccines

     None




2                                                 Annual reports of OIE Reference Laboratories and Collaborating Centres, 2008
                                                          Salmonellosis




4.   Preparation and supply of international reference standards for diagnostic tests or vaccines

     None


5.   Research and development of new procedures for diagnosis and control

       Nr.            Designation of method
       1              Multilocus Variable-Number Tandem-Repeats (VNTR) Analysis for subtyping Salmonella
                      enterica Serovars (Generic)
       2              Characterization of Salmonella serovars by DNA microarrays


6.   Collection, analysis and dissemination of epizootiological data relevant to international disease
     control

     The unit 'Epidemiology and Zoonoses' is collecting the results of import control of feeding stuffs (originating
     mostly from South America) as part of the national zoonoses surveillance on food, animals and feeding stuffs.
     This information is summarised in the annual German report on the trend and sources of zoonoses and zoonotic
     infections and submitted to the European Commission according to EU-Zoonoses directive (2003/99/EC) (cf.
     http://www.bfr.bund.de/ cd/299).

     Three control programmes, as described in Council Regulations (EC) No 1168/2006, 516/2007 and 584/2008,
     were run in 2010 to estimate the prevalence of Salmonella in flocks of laying hens, broiler flocks and turkey
     flocks. They were run between January and December 2010. Faecal samples from laying hens, broilers and
     turkeys as well as dust from houses of hens were investigated by ISO 6579 Annex D. For each flock two faecal
     samples (2 pairs of boot swabs and in case of hens 250 ml dust) were used for analysis.

     The outcome of the regular surveillance, which is carried out in each country of the EU will be used to assess the
     progress of the control strategy, which has been implemented.


7.   Provision of consultant expertise to OIE or to OIE Member Countries and Territories

     None


8.   Provision of scientific and technical training to personnel from other OIE Member Countries and
     Territories

     None


9.   Provision of diagnostic testing facilities to other OIE Member Countries and Territories

     None


10. Organisation of international scientific meetings on behalf of OIE or other international bodies

     None


11. Participation in international scientific collaborative studies

     The NRL-Salm has participated in the following ring-trials. They were organized by the EURL-Salm (Bilthoven,
     The Netherlands) within the frame of quality control studies of National Salmonella Reference Laboratories, and
     the EURL-Antimicrobial Resistance (DTU, Lingby, Denmark).




Annual reports of OIE Reference Laboratories and Collaborating Centres, 2008                                         3
                                                    Salmonellosis




         Time              Ring-trial                Organisation                  Detection of                 Matrix

                     Salmonella detection in       EURL-Salmonella
     15/03/2010                                                                      Salmonella                 Faeces
                             feces                    Bilthoven

                     Salmonella detection in      EURL-Salm RIVM                    Salmonella
     27/09/2010                                                                                                  Food
                             food                    Bilthoven                  (Cultural and PCR)

                     Serotyping and phage         EURL-Salm RIVM              Salmonella serovars and          Bacterial
     15/11/2010
                            typing                   Bilthoven                     phage types                  culture

                  Antimicrobial susceptibility
                                                    EURL-AR DTU                 E. coli, Enterococci,          Bacterial
     30.07.2010     testing; Detection of ß-
                                                       Lingby                      Staphylococci                culture
                           lactamases

                  Antimicrobial susceptibility
                            testing
                                                    EURL-AR DTU                                                Bacterial
     18.12.2010    Detection of ß-lactamases                                         Salmonella
                                                       Lingby                                                   culture
                    Detecction of resistance
                             genes


                            The NRL-Salm achieved in all ring-trials excellent performance.


12. Publication and dissemination of information relevant to the work of OIE (including list of
    scientific publications, internet publishing activities, presentations at international conferences)

        Presentations at international conferences and meetings

    1.   Beutlich, J, Rodicio, M. R., Mendoza, M. C., Kirchner, M., Luzzi, I., Mevius, D., Threlfall, J., Helmuth, R.,
         Guerra, B., and on behalf of the WP21 Project group. Salmonella enterica subsp. enterica serovar
         Typhimurium isolates collected within the European Project Med-Vet-Net (Workpackage 21) carry
         Virulence-Resistance plasmids derived from the pSLT90. Proceedings of the 13S International Symposium
         Salmonella and Salmonellosis, June 2010, Saint-Malo – France. 2010. Saint-Malo, France. Ref Type:
         Conference Speaker

    2.   Beutlich, J, Rodicio, M. R., Mendoza, M. C., Kirchner, M., Luzzi, I., Mevius, D., Threlfall, J., Helmuth, R.,
         Guerra, B., and on behalf of the WP21 Project group. Salmonella enterica subsp. enterica serovar
         Typhimurium isolates collected within the European Project Med-Vet-Net (Workpackage 21) carry
         Virulence-Resistance plasmids derived from the pSLT90. 13S International Symposium Salmonella and
         Salmonellosis, June 2010, Saint-Malo - France. 2010. Saint-Malo, France. Ref Type: Conference Proceeding

    3.   Beutlich, J, Jaber, M, Thomas, K., Schroeter, A., Granier, S. A., Herrera-León, S., Hopkins, K., Luzzi, I.,
         Erzsebet, A., Szych, J., Torpdahl, M., Veldman, K., Wu, G., Appel, B., Threlfall, E. J., Mevius, D., Helmuth,
         R., and Guerra, B. Salmonella Genomic Island 1 variants in Salmonella enterica subsp. enterica serovar
         Newport isolates collected within the European Project Med-Vet-Net (WP21). 13S International Symposium
         Salmonella and Salmonellosis, June 2010, Saint-Malo - France. 2010. Saint-Malo, France. Ref Type:
         Conference Proceeding

    4.   Beutlich, J, Jaber, M, Thomas, K., Schroeter, A., Granier, S. A., Herrera-León, S., Hopkins, K., Luzzi, I.,
         Erzsebet, A., Szych, J., Torpdahl, M., Veldman, K., Wu, G., Appel, B., Threlfall, E. J., Mevius, D., Helmuth,
         R., and Guerra, B. Salmonella Genomic Island 1 variants in Salmonella enterica subsp. enterica serovar
         Newport isolates collected within the European Project Med-Vet-Net (WP21). 13S International Symposium
         Salmonella and Salmonellosis, June 2010, Saint-Malo - France. 2010. Saint-Malo, France. Ref Type: Poster

    5.   Beutlich, J, Malorny, B., Huehn, S., Schroeter, A., Amar, C., Appel, B., Threlfall, E. J., Mevius, D.,
         Helmuth, R, and Guerra, B. Antimicrobial resistance and virulotyping of "Salmonella Genomic island 1"




4                                                Annual reports of OIE Reference Laboratories and Collaborating Centres, 2008
                                                          Salmonellosis




           positive strains collected within the European Med-Vet-Net project. 9th International Meeting on Microbial
           Epidemiological Markers IMMEM. 2010. Wernigerode. Ref Type: Poster

     6.    Beutlich, J, Malorny, B., Huehn, S., Schroeter, A., Amar, C., Appel, B., Threlfall, E. J., Mevius, D.,
           Helmuth, R, and Guerra, B. Antimicrobial resistance and virulotyping of "Salmonella Genomic island 1"
           positive strains collected within the European Med-Vet-Net project. 9th International Meeting on Microbial
           Epidemiological Markers IMMEM. 2010. Wernigerode. Ref Type: Conference Proceeding

     7.    Dieckmann, R, Helmuth, R, and Malorny, M. Rapid screening of epidemiologically important Salmonella
           enterica subsp. enterica serovars using Whole-Cell MALDI-TOF mass spectrometry. National Symposium
           on Zoonoses Research. 7-10-2010. Berlin, National Symposium on Zoonoses Research 2010, Programme
           and Abstracts. 7-10-2010. Ref Type: Conference Proceeding

     8.    Dieckmann, R. Schnelle MALDI-TOF MS-basierte Identifizierung von Bakterien. 2010. BFR Seminar,
           Bundesinstitut für Risikobewertung, Berlin, Deutschland. 3-2-2010. Ref Type: Conference Speaker

     9.    García-Miura, L., Karlsmose, S., Veldman, K. M Schroeter A., Guerra, B., Sunde, M, Granier, S. A., Perrin-
           Guyomard, Gicquel-Bruneu, M., Donati, V., Englund, S., Teale, C., Myllyniemi, A-L., Clemente, L.,
           Boerlin, P., Moreno M.A., Daignault, D., Mevius, D., Franco, A., Marchant, M., Hendriksen, R. S., and
           Aarestrup, F. M. Establishing streptomycin epidemiological cut-off values for Salmonella and Escherichia
           coli . 2nd ASM Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens in
           Animals, Humans and the Environment. 2010. Toronto, Canada. 2010. Ref Type: Poster

     10. García-Miura, L., Karlsmose, S., Veldman, K. M Schroeter A., Guerra, B., Sunde, M, Granier, S. A., Perrin-
         Guyomard, Gicquel-Bruneu, M., Donati, V., Englund, S., Teale, C., Myllyniemi, A-L., Clemente, L.,
         Boerlin, P., Moreno M.A., Daignault, D., Mevius, D., Franco, A., Marchant, M., Hendriksen, R. S., and
         Aarestrup, F. M. Establishing streptomycin epidemiological cut-off values for Salmonella and Escherichia
         coli . 2nd ASM Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens in
         Animals, Humans and the Environment. 2010. Toronto, Canada. 2010. Ref Type: Conference Proceeding

     11. Guerra, B., Thomas, K., Beutlich, J, Jahn, S., Cavaco, L., Veldman, K., Helmuth, R., and Schroeter A.
         Presence of different resistance determinants conferring plasmid-mediated quinolone resistance in
         Salmonella isolates from reptiles in Germany. 20th European Congress of Clinical Microbiology and
         Infectious Diseases, ECCMID. 2010. Vienna, Austria. 10-4-2010. Ref Type: Conference Proceeding

     12. Guerra, B., Thomas, K., Beutlich, J, Jahn, S., Cavaco, L., Veldman, K., Helmuth, R., and Schroeter A.
         Presence of different resistance determinants conferring plasmid-mediated quinolone resistance in
         Salmonella isolates from reptiles in Germany. 20th European Congress of Clinical Microbiology and
         Infectious Diseases, ECCMID. 2010. Vienna, Austria. 10-4-2010. Ref Type: Poster

     13. Hauser, E., Tietze, E., Prager, R., Helmuth, R, and Malorny, B. Molecular characterization of Salmonella
         enterica serovar 4,[5],12:i:- from pig via pork to human. 3rd Joint Conference of DGHM and VAAM.
         Convention Center, Hannover, Germany. 1928. Ref Type: Poster

     14. Hauser, E., Hebner, F., Tietze, E., Helmuth, R, Junker, E., Prager, R., Fruth, A., and Malorny, B. Clonal
         spread of Salmonella enterica serovar Derby from pigs to humans in Germany. National Symposium on
         Zoonoses Research 2010. Berlin. 2010. Ref Type: Poster

     15. Hauser, E., Hebner, F., Tietze, E., Helmuth, R, Junker, E., Prager, R., Fruth, A., and Malorny, B. Clonal
         spread of Salmonella enterica serovar Derby from pigs to humans in Germany. National Symposium on
         Zoonoses Research 2010. 2010. Berlin, National Symposium on Zoonoses Research 2010, Programme and
         Abstracts. Ref Type: Conference Proceeding

     16. Hauser, E., Tietze, E., and Malorny, B. Molecular characterization of Salmonella enterica serovar 4,[5],12:i:-
         from pig via pork to human. 3rd Joint Conference of DGHM and VAAM. 2010. Hannover. Ref Type:
         Conference Proceeding

     17. Hauser, E. and Malorny, B. Mikroarray "Theorie und Praxis". FBI-Zoo DNA-Mikroarray Workshop, BfR,
         Berlin. 2010. Berlin. 20-4-2010. Ref Type: Conference Proceeding




Annual reports of OIE Reference Laboratories and Collaborating Centres, 2008                                         5
                                                   Salmonellosis




    18. Hauser, E. Mutationen im oafA Gen führen zum Verlust der O5-Antigen Expression bei Salmonella enterica
        serovar Typhimurium. 7. Statustreffen FBI-Zoo-Verbund. 2010. Tierärztlich Hochschule Hannover. 1904.
        Ref Type: Conference Proceeding

    19. Hebner, F., Hauser, E., Malorny, B., and Tietze, E. A novel molecular typing approach for Salmonella
        enterica Serovar Derby based on virulence genes and tandem repeat analyses. 3rd Joint Conference of
        DGHM and VAAM. Convention Center, Hannover, Germany. 2010. Ref Type: Poster

    20. Hopkins, K., Nair, S., Kirchner, M., Guerra, B., Granier, S. A., Luzzi, I., Porrero, C., Jakubczak, A.,
        Threlfall, E. J., and Mevius, D. Generic variation in emerging Salmonella enterica serotype 4,[5],12:i:- from
        seven European countries. 2nd ASM Conference on Antimicrobial Resistance in Zoonotic Bacteria and
        Foodborne Pathogens in Animals, Humans and the Environment. 2010. Toronto, Canada. 2010. Ref Type:
        Poster

    21. Hopkins, K., Nair, S., Kirchner, M., Guerra, B., Granier, S. A., Luzzi, I., Porrero, C., Jakubczak, A.,
        Threlfall, E. J., and Mevius, D. Generic variation in emerging Salmonella enterica serotype 4,[5],12:i:- from
        seven European countries. 2nd ASM Conference on Antimicrobial Resistance in Zoonotic Bacteria and
        Foodborne Pathogens in Animals, Humans and the Environment. 2010. Toronto, Canada. 2010. Ref Type:
        Conference Proceeding

    22. Löfström, C., Grønlund, H., Riber, L., Håkan, V., Folling, L., Huehn, S., Malorny, B., Rådström, P., Rudi,
        K., and Hoorfar, J. Towards standardization of microarray-based genotyping of Salmonella. 22nd
        International ICFMH Symposium, Food Micro 2010. Bella Center, Copenhagen, Denmark. 1930. Ref Type:
        Poster

    23. Löfström, C., Grønlund, H., Riber, L., Håkan, V., Folling, L., Huehn, S., Malorny, B., Rådström, P., Rudi,
        K., and Hoorfar, J. Towards standardization of microarray-based genotyping of Salmonella. Biotracer 4th
        General Meeting, 22.-23. march 2010. DTU National Food Institute, Copenhagen, Denmark. 1930. Ref Type:
        Poster

    24. Löfström, C., Grønlund, H., Riber, L., Håkan, V., Folling, L., Huehn, S., Malorny, B., Rådström, P., Rudi,
        K., and Hoorfar, J. Towards standardization of microarray-based genotyping of Salmonella. 4th Biotracer
        General Meeting. 2010. Danish Technical Univerity, Copenhagen, Denmark. 3-3-1922. Ref Type:
        Conference Proceeding

    25. Malorny, B. Characteristics of two increasingly isolated Salmonella serovars 4,[5],12:i:- and Paratyphi B (d-
        tartrate +). 6. FBI-Zoo Statusmeeting. 2010. Veterinarium Progressum, FU-Berlin, Berlin. 4-4-1922. Ref
        Type: Conference Speaker

    26. Malorny, B. Salmonella strain typing through the decades: Do new methods give better information than
        traditional methods? Colloquium des Instituts für Lebensmittelsicherheit und -hygiene der Universität Zürich.
        2010. Institut für Lebensmittelsicherheit und -hygiene der Universität Zürich, Zürich, Schweiz. 11-5-2010.
        Ref Type: Conference Speaker

    27. Malorny, B. and Hauser, E. Aufbau des Salmonella Genoms. FBI-Zoo DNA-Mikroarray Workshop, BfR,
        Berlin. 2010. Berlin. 20-4-2010. Ref Type: Conference Speaker

    28. Moury, F., Fremy, S., van Santen, R., Imberechts, H., Giles, M., Malorny, B., Clerico Lera, M., Maas, H.,
        Fabre, J. M., and Brisabois, A. Interlaboratory study of a DNA Microarray for Salmonella molecular
        serotyping in comparison to the conventional serotyping method. International Symposium Salmonella and
        Salmonellosis. 2010. Palais du Grant Large, Saint-Malo, France. 6-6-1927. Ref Type: Poster

    29. Moury, F., Fremy, S., van Santen, R., Imberechts, H., Giles, M., Malorny, B., Clerico Lera, M., Maas, H.,
        Fabre, J. M., and Brisabois, A. Interlaboratory study of a DNA Microarray for Salmonella molecular
        serotyping in comparison to the conventional serotyping method. 19th European Congress of Clinical
        Microbiology and Infectious Diseases, ECCMID. 2010. Palais du Grant Large, Saint-Malo, France. 6-6-
        1927. Ref Type: Poster

    30. Rodriguez, I., García, P., Montero, I., Herrero, A., Martinez, N., Beutlich, J., Martin, M. C., Rodicio, R.,
        Guerra, B., Mendoza, M. C., and Rodicio, M. R. Tn21-like transposons found in non-typhoidal Salmonella




6                                               Annual reports of OIE Reference Laboratories and Collaborating Centres, 2008
                                                          Salmonellosis




           enterica isolates of clinical origin carrying class 1 integrons. 20th European Congress of Clinical
           Microbiology and Infectious Diseases, ECCMID. 2010. Vienna, Austria. 10-4-2010. Ref Type: Conference
           Proceeding

     31. Rodriguez, I., García, P., Montero, I., Herrero, A., Martinez, N., Beutlich, J., Martin, M. C., Rodicio, R.,
         Guerra, B., Mendoza, M. C., and Rodicio, M. R. Tn21-like transposons found in non-typhoidal Salmonella
         enterica isolates of clinical origin carrying class 1 integrons. 20th European Congress of Clinical
         Microbiology and Infectious Diseases, ECCMID. 2010. Vienna, Austria. 10-4-2010. Ref Type: Poster

     32. Rodriguez, I., Jahn, S., Bahl, J., Schroeter, A., Käsbohrer, A., Helmuth, R., and Guerra, B. Detection of qnrC
         in two ESBL-positive Escherichia coli isolates originated from animals in Germany. 9th International
         Meeting on Microbial Epidemiological Markers IMMEM. 2010. Wernigerode. Ref Type: Poster

     33. Rodriguez, I., Jahn, S., Bahl, J., Schroeter, A., Käsbohrer, A., Helmuth, R., and Guerra, B. Detection of qnrC
         in two ESBL-positive Escherichia coli isolates originated from animals in Germany. 9th International
         Meeting on Microbial Epidemiological Markers IMMEM. 2010. Wernigerode. Ref Type: Conference
         Proceeding

     34. Heckenbach, K., Dorn, C., Schroeter, A., and Käsbohrer, A. Spread of Salmonella spp. in broiler
         slaughterhouses in Germany. International Symposium Salmonella and Salmonellosis. 2010. St. Malo, FR.
         28-6-2010.

     35. Käsbohrer, A., Schroeter, A., Dorn, C., Heckenbach, K., Tenhagen, B.-A., Helmuth, R., and Appel, B.
         Resistenzmonitoring bei Zoonoseerregern und Kommensalen in der Lebensmittelkette. DVG-Fachgruppe
         "Bakteriologie und Mykologie". 2010. Jena. 22-6-2010.

     36. Käsbohrer, A., Schroeter, A., Dorn, C., Heckenbach, K., Tenhagen, B.-A., Helmuth, R., and Appel, B.
         Monitoring of antimicrobial resistance in Salmonella spp. in Germany. Nationales Symposium für
         Zoonosenforschung. 2010. Berlin. 7-10-2010.

     37. Käsbohrer, A., Tenhagen, B.-A., Heckenbach, K., Dorn, C., Schroeter, A., and Helmuth, R. Salmonellen
         beim Geflügel - Ausgangssituation für die amtlichen Bekämpfungsprogramme. Leipziger Blaue Hefte. 274-
         278. 2010. Leipzig, Leipziger Universitätsverlag GmbH. LBH: Proceedings 5. Leipziger Tierärztekongress.
         21-1-2010.

     38. Schroeter, A., Dorn, C., Käsbohrer, A., and Helmuth, R. Untersuchungen zur Resistenz von Salmonella-
         Isolaten vom Schwein in Deutschland. DVG-Fachgruppe "Bakteriologie und Mykologie"
         Fachgruppentagung Jena. 2010. Friedrich-Loeffler-Institut (FLI), Jena. 22-6-2010.

     39. Schroeter, A., Käsbohrer, A., Helmuth, R., Dorn, C., Heckenbach, K., and Tenhagen, B.-A. Resistance
         patterns of Salmonella isolated from pigs and porc in Germany. 21st IPVS Congress. 2010. Vancouver,
         Canada . 18-6-2010

     40. Veldman, K., Cavaco, L. M., Mevius, D., Battisti, A., Cerny, T., Franco, A., De Frutos Escobar, C., Guerra,
         B., Gutierrez, M., Heylen, K., Hopkins, K., Myllyniemi, A. L., Perrin-Guyomard, A., Schroeter, A., Sunde,
         M., Wasyl, D., and Aarestrup, F. M. International collaborative study on the prevalence of plasmid mediated
         quinolone resistance (PMQR) in Salmonella and E. coli isolated from humans and animals in Europe. 2nd
         ASM Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens in Animals,
         Humans and the Environment. 2010. Toronto, Canada. 2010. Ref Type: Conference Proceeding

     41. Veldman, K., Cavaco, L. M., Mevius, D., Battisti, A., Cerny, T., Franco, A., De Frutos Escobar, C., Guerra,
         B., Gutierrez, M., Heylen, K., Hopkins, K., Myllyniemi, A. L., Perrin-Guyomard, A., Schroeter, A., Sunde,
         M., Wasyl, D., and Aarestrup, F. M. International collaborative study on the prevalence of plasmid mediated
         quinolone resistance (PMQR) in Salmonella and E. coli isolated from humans and animals in Europe. 2nd
         ASM Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens in Animals,
         Humans and the Environment. 2010. Toronto, Canada. 2010. Ref Type: Poster

         Scientific publications in peer-reviewed journals

     1.    Beutlich, J., Rodriguez, I., Schroeter, A., Käsbohrer, A., Helmuth, R., and Guerra, B. (2010). A predominant



Annual reports of OIE Reference Laboratories and Collaborating Centres, 2008                                         7
                                                    Salmonellosis




         multidrug-resistant Salmonella enterica serovar Saintpaul clonal line in German turkey and related food
         products. Appl Environ Microbiol 76, 3657-3667.

    2.   Ellerbroek, L., Narapati, D., Phu, T.N., Poosaran, N., Pinthong, R., Sirimalaisuwan, A., Tshering, P., Fries,
         R., Zessin, K.H., Baumann, M., and Schroeter, A. (2010). Antibiotic resistance in salmonella isolates from
         imported chicken carcasses in bhutan and from pig carcasses in Vietnam. J Food Prot 73, 376-379.

    3.   Friedrich, A., Dorn, C., Schroeter, A., Szabo, I., Jaber, M., Berendonk, G., Brom, M., Ledwolorz, J., and
         Helmuth, R. (2010). Bericht des Nationalen Referenzlabors zur Durchführung von Analysen und Tests auf
         Zoonosen (Salmonellen) zum Vorkommen von Salmonellen in Nutztieren, Lebens- und Futtermitteln über
         den Zeitraum der letzten fünf Jahre in Deutschland (2004–2008). Berl Münch. Tierärztl. Wochenschr. 123,
         265-277.

    4.   Garcia, P., Guerra, B., Bances, M., Mendoza, M.C., and Rodicio, M.R. (2010). IncA/C plasmids mediate
         antimicrobial resistance linked to virulence genes in the Spanish clone of the emerging Salmonella enterica
         serotype 4,[5],12:i:-. J Antimicrob. Chemother.

    5.   Grønlund, H., Riber, L., Vigre, H., Löfström, C., Folling, L., Huehn, S., Malorny, B., Rådström, P., Rudi, K.,
         and Hoorfar, J. (2010). Microarray-based genotyping of Salmonella: Inter-laboratory evaluation of
         reproducibility and standardization potential. Int J Food Microbiol (in press).

    6.   Guerra, B., Helmuth, R., Thomas, K., Beutlich, J., Jahn, S., and Schroeter, A. (2010). Plasmid-mediated
         quinolone resistance determinants in Salmonella spp. isolates from reptiles in Germany. J Antimicrob.
         Chemother. 65, 2043-2045.

    7.   Hammerl, J.A., Beutlich, J., Hertwig, S., Mevius, D., Threlfall, E.J., Helmuth, R., and Guerra, B. (2010).
         pSGI15, a small ColE-like qnrB19 plasmid of a Salmonella enterica serovar Typhimurium strain carrying
         Salmonella genomic island 1 (SGI1). J Antimicrob. Chemother. 65, 173-175.

    8.   Hartung, M. and A. Käsbohrer (2010): Results of the Zoonoses Surveillance 2009 in foodstuffs in Germany
         (German with English Summary). Fleischwirtschaft 11/2010: 116-122

    9.   Hauser, E., Tietze, E., Helmuth, R., Junker, E., Blank, K., Prager, R., Rabsch, W., Appel, B., Fruth, A., and
         Malorny, B. (2010). Pork contaminated with Salmonella enterica serovar 4,[5],12:i:-, an emerging health risk
         for humans. Appl Environ Microbiol 76, 4601-4610.

    10. Hauser, E., Junker, E., Helmuth, R., and Malorny, B. (2010). Different mutations in the oafA gene lead to
        loss of O5-antigen expression in Salmonella enterica serovar Typhimurium. Journal of Applied
        Microbiology 110, 248-253.

    11. Hopkins, K.L., Kirchner, M., Guerra, B., Granier, S.A., Lucarelli, C., Porrero, M.C., Jakubczak, A., Threlfall,
        E.J., and Mevius, D.J. (2010). Multiresistant Salmonella enterica serovar 4,[5],12:i:- in Europe: a new
        pandemic strain? Euro. Surveill 15, 19580.

    12. Huehn, S., La Ragione, R.M., Anjum, M., Saunders, M., Woodward, M.J., Bunge, C., Helmuth, R., Hauser,
        E., Guerra, B., Beutlich, J., Brisabois, A., Peters, T., Svensson, L., Madajczak, G., Litrup, E., Imre, A.,
        Herrera-Leon, S., Mevius, D., Newell, D.G., and Malorny, B. (2010). Virulotyping and antimicrobial
        resistance typing of Salmonella enterica serovars relevant to human health in Europe. Foodborne Pathog. Dis
        7 , 523-535.

    13. Krämer, N., Löfström, C., Vigre, H., Hoorfar, J., Bunge, C., and Malorny, B. (2010). A novel strategy to
        obtain quantitative data for modelling: Combined enrichment and real-time PCR for enumeration of
        salmonellae from pig carcasses. Int J Food Microbiol in press.

    14. Litrup, E., Torpdahl, M., Malorny, B., Huehn, S., Helms, M., Christensen, H., and Nielsen, E.M. (2010).
        DNA microarray analysis of Salmonella serotype Typhimurium strains causing different symptoms of
        disease. BMC Microbiol. 10, 96.

    15. Litrup, E., Torpdahl, M., Malorny, B., Huehn, S., Christensen, H., and Nielsen, E.M. (2010). Association
        between phylogeny, virulence potential and serovars of Salmonella enterica. Infect Genet Evol. 10, 1132-



8                                                Annual reports of OIE Reference Laboratories and Collaborating Centres, 2008
                                                          Salmonellosis




           1139.

     16. Rodriguez, I., Guerra, B., Mendoza, M.C., and Rodicio, M.R. (2011). pUO-SeVR1 is an emergent virulence-
         resistance complex plasmid of Salmonella enterica serovar Enteritidis. J Antimicrob. Chemother. 66, 218-
         220.

     17. Sangal, V., Harbottle, H., Mazzoni, C.J., Helmuth, R., Guerra, B., Didelot, X., Paglietti, B., Rabsch, W.,
         Brisse, S., Weill, F.X., Roumagnac, P., and Achtman, M. (2010). Evolution and population structure of
         Salmonella enterica serovar Newport. J Bacteriol. 192, 6465-6476.

          Other communications

     1.    Schroeter, A., B.-A. Tenhagen, K. Heckenbach, B. Guerra, R. Helmuth, J. Beutlich, A. Hensel, B. Appel, and
           A. Käsbohrer. 2010. Deutsche Antibiotika-Resistenzsituation in der Lebensmittelkette - DARLink.
           Salmonella 2000-2008, p. 335pp. Bundesinstitut für Risikobewertung, Berlin.

     2.    HARTUNG, M. (2010): Erreger von Zoonosen in Deutschland im Jahr 2008. BfR-Wissenschaft 6/2010, 234
           S., 34 Abb., 80 Tab.


13. Inscription of diagnostic kits on the OIE Register

     i)    Did you participate in expert panels for the validation of candidate kits for inscription on the
           OIE Register? If yes, for which kits?

           None

     ii)   Did you submit to the OIE candidate kits for inscription on the OIE Register? If yes, for
           which kits?

           None


                                                       _______________




Annual reports of OIE Reference Laboratories and Collaborating Centres, 2008                                       9

				
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