6- Metabolism . ppt by CJhb7c3

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									Bacterial Growth and Metabolism
     -- Chapter 9, & part of Chapter 14

Learn on your own the
materials in the section growth:
     -- Population growth
     -- Water and temperature
     -- Oxygen and acidity
     -- Other factors
(Much of the chapter Self-test
 focuses on these topics)

Also read section in Chap 14 on
Microbes and Industry


*** You will not be responsible for all of the
details of the metabolic pathways covered in
this chapter ***



                                  Bacterial Growth and Metabolism   1
Bacterial Growth and Metabolism
    -- Chapter 9


All organisms have
three fundamental requirements

    Carbon

    Energy

    Mineral nutrients




                          Bacterial Growth and Metabolism   2
Prokaryotes are much more varied
in their sources of C and energy

Bacterial autotrophs

  Phototrophs
      -- photosynthetic bacteria

  Chemoautotrophs
   “Lithotrophs”
      -- mineral oxidizing bacteria




                                   Bacterial Growth and Metabolism   3
Prokaryotes are much more varied
in their sources of C and energy
Bacterial heterotrophs
  Photoheterotrophs
  Chemoheterotrophs
     saprophytic
     parasitic

Generalists vs   fastidious bacteria




                             Bacterial Growth and Metabolism   4
Use of Microbes in Bioremediation


Toxic waste sites:
    PCBs, dioxins, organic solvents, etc

Traditional clean up is expensive & inefficient
     (10’s to 100’s $M)

Bioremediation is better alternative
     Less impact

    Cheaper

    Does not merely move the problem




                             Bacterial Growth and Metabolism   5
Cellular Metabolism

Why is this topic of importance?                  Overview of Cellular Respiration

  identification

  microbial application

  chemotherapies


What happens to organic molecules
that enter a cell?

  Enzymatically degraded

  molecular building blocks

  energy retention




                              Bacterial Growth and Metabolism                        6
Glycolysis

Converts sugars into Pyruvate


Requires energy investment


Energy yield

  ATP

  NADH




Glycolysis
                         Bacterial Growth and Metabolism   7
Aerobic Cellular Respiration
Transition reaction


Krebs cycle


Electron transport


Energy yield

  ATP

  NADH




   e- Transport


                      Bacterial Growth and Metabolism   8
Anaerobic metabolism
Bacterial O2 requirements
    Obligate aerobes

    Facultative

    Microaerophiles

    Obligate anaerobes

Fermentation

    Functions

    End products


Alternative terminal e- acceptors

e.g., CO2, SO4



                            Bacterial Growth and Metabolism   9
Industrial Uses of Bacteria
    -- chapter 14

                                                           Figure 5.12
Other fermentation products



Antibiotics and
pharmaceutics




                         Bacterial Growth and Metabolism            10
Beer production
Normal beer: only yeast
    … boring!

Lambic beer … yeast and bacteria!


         Yeast CO2 production




                                Bacterial Growth and Metabolism   11
Cheese production
     Involves bacteria (and sometimes fungi)

Curdling stage



Ripening stage




                                   Bacterial Growth and Metabolism   12
Autotrophic bacteria
  CO2 is carbon source
  Energy from light (phototrophs)
    or inorganics (chemoautotrophs)

Phototrophs

Purple sulfur bacteria
  -- anoxygenic Ps

Cyanobacteria
  -- oxygenic Ps




                            Bacterial Growth and Metabolism   13
Cyanobacteria
“blue-green” pigmentation

Habitat                                                        Visit NASA’a
                                                               “Stromatolite
Stromatolites                                                 Explorer” on-line




Oxygenic
Photosynthesis




                            Bacterial Growth and Metabolism                  14
Purple sulfur bacteria
Photosynthesis

Habitat

Pigmentation                          Anoxygenic
                                      Photosynthesis

Sulfur granules




                    Bacterial Growth and Metabolism    15

								
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