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Chemistry Pre-laboratory Tutorials
Written By Patrick Herklotz
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Tutorial 4
Calibrating and Operating the Vernier pH Sensor
Tutorial 4
Calibrating and Operating the Vernier pH Sensor
Previous Slide
Replay
Continue
Previous Slide
Step 1: Assemble the LabPro Calculator as explained in
Tutorial 1 Replay
Continue
Top View Power Supply
Quick Setup
Transfer Start/Stop
VERNIER
LabPro
TBLSET F2 FORNAT F3 CALC F4
DEL
STAT
PRGM
COS
(
8
5
2
.
Vernier
Previous Slide
Step 1: Assemble the LabPro Calculator as explained in
Tutorial 1. Replay
Continue
Top View Power Supply
Quick Setup
Transfer Start/Stop
VERNIER
LabPro
TBLSET F2 FORNAT F3 CALC F4
DEL
STAT
PRGM
COS
(
8
5
2
.
Vernier
Previous Slide
Step 2: Obtain the pH sensor and plug it into channel 1.
Run the DataMate program by selecting it from the APPS Replay
menu in order to allow the calculator to detect the pH Continue
sensor.
Top View Power Supply
Quick Setup
Transfer Start/Stop
VERNIER
LabPro
TBLSET F2 FORNAT F3 CALC F4
DEL
STAT
PRGM
COS
(
8
5
2
.
Vernier
Channel 1 Channel 2 Channel 3 Channel 4
Side View
Previous Slide
Step 2: Obtain the pH sensor and plug it into channel 1.
Run the DataMate program by selecting it from the APPS Replay
menu in order to allow the calculator to detect the pH Continue
sensor.
Top View Power Supply
Quick Setup
Transfer Start/Stop
Checking Sensors
CH 1: Absorbance
VERNIER SOFTWARE 0.475
VERNIER
DATAMATE
LabPro
Mode: TIME GRAPH-180
(VER 1.15)
1: SETUP 4: ANALYZE
2: START 5: TOOLS
ROM 6.27 6: QUIT (C)2003
3: GRAPH
TBLSET F2 FORNAT F3 CALC F4
pH SENSOR
DEL
STAT
PRGM
COS
(
8
5
2
.
Vernier
Channel 1 Channel 2 Channel 3 Channel 4
Channel 1 Port
Side View
Previous Slide
Step 3: In the default DataMate program screen, press the 1
key (SETUP). In the next menu, press 2 (CALIBRATE) once the Replay
cursor triangle is to the left of CH 1. In the third menu, press the
Continue
2 key (CALIBRATE NOW).
Power Supply
Quick Setup
Transfer Start/Stop
VERNIER
LabPro
TBLSET F2 FORNAT F3 CALC F4
pH SENSOR
DEL
STAT
PRGM
COS
(
8
5
2
.
Vernier
Texas Instruments
TI-84 Silver Edition
CH 1: PH
Mode: TIME GRAPH-180 LabPro Calculator
1: SETUP 4: ANALYZE Screen
2: START 5: TOOLS
3: GRAPH 6: QUIT
Previous Slide
Step 3: In the default DataMate program screen, press the 1
key (SETUP). In the next menu, press 2 (CALIBRATE) once the Replay
cursor triangle is to the left of CH 1. In the third menu, press the
Continue
2 key (CALIBRATE NOW).
Power Supply
Quick Setup
Transfer Start/Stop
VERNIER
LabPro
TBLSET F2 FORNAT F3 CALC F4
pH SENSOR
DEL
STAT
PRGM
COS
(
8
5
2
.
Vernier
Texas Instruments
TI-84 Silver Edition
CALIBRATION
CH 1:1:CALIBRATION SENSOR
CH PH PH
CH 2:
PHCH 3: VOLTAGE, WHEN
MONITOR
CALIBRATION: LINEAR
CH 4:
STABLE,PRESS [ENTER].
SLOPE
DIG1:
0 DIG2:
INT VALUE
Mode: TIME GRAPH-180 VOLTAGE
4
LabPro Calculator
POINT 1: 2.627
POINT 2: TIME GRAPH-900
MODE:
1: SETUP
1:OK 4: ANALYZE Screen
2:CALIBRATE NOW 5: TOOLS
2: START
1:OK 3:ZERO
3: GRAPH ENTRY 6: QUIT 4:SAVE/LOAD
2:CALIBRATE
3:MANUAL
Step 4: Remove the pH sensor from the solution bottle that Previous Slide
keeps it moist. Thoroughly rinse the pH electrode with DI water Replay
over a beaker. Always perform this step before measuring the
pH of a new solution. Use a Chemwipe to gently rub off strong Continue
bases from the sensor’s electrode after rinsing it with DI water.
Power Supply
Quick Setup
Transfer Start/Stop
VERNIER
LabPro
SENSOR
pH
TBLSET F2 FORNAT F3 CALC F4
300
- STAT
DEL
250 PRGM
COS
150 (
8
5
100
2
.
50
0 ml
Vernier
Texas Instruments
TI-84 Silver Edition
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE LabPro Calculator
POINT 1: 2.627
POINT 2: Screen
Step 4: Remove the pH sensor from the solution bottle that Previous Slide
keeps it moist. Thoroughly rinse the pH electrode with DI water Replay
over a beaker. Always perform this step before measuring the
pH of a new solution. Use a Chemwipe to gently rub off strong Continue
bases from the sensor’s electrode after rinsing it with DI water.
Power Supply
Quick Setup
Transfer Start/Stop
VERNIER
LabPro
SENSOR
pH
TBLSET F2 FORNAT F3 CALC F4
300
Rinse pH electrode - DEL
with DI water from
STAT
250 PRGM
150
lab squeeze bottles. COS
(
8
5
100
2
.
50
0 ml
Vernier
Texas Instruments
TI-84 Silver Edition
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE LabPro Calculator
POINT 1: 2.627
POINT 2: Screen
Step 5: Place the sensor electrode into a buffer that has a pH of 4. Previous Slide
Allow the voltage to stabilize to a reasonably constant value out to the
hundredth place past the decimal. This may take 10-15 seconds. Replay
Once this occurs, press the ENTER key. On the next screen, press
the 4 key to input the pH of this buffer into the calculators memory. Continue
Press the ENTER Key again.
Power Supply
Quick Setup
Transfer Start/Stop
VERNIER
LabPro
SENSOR
pH
TBLSET F2 FORNAT F3 CALC F4
300 - STAT
DEL
PRGM
250 COS
(
150 8
5
100 2
.
50
0 ml
Vernier
Texas Instruments
TI-84 Silver Edition
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE LabPro Calculator
POINT 1:
POINT 2: Screen
Step 5: Place the sensor electrode into a buffer that has a pH of 4. Previous Slide
Allow the voltage to stabilize to a reasonably constant value out to the
hundredth place past the decimal. This may take 10-15 seconds. Replay
Once this occurs, press the ENTER key. On the next screen, press
the 4 key to input the pH of this buffer into the calculators memory. Continue
Press the ENTER Key again.
Power Supply
Quick Setup
Transfer Start/Stop
VERNIER
LabPro
SENSOR
pH
TBLSET F2 FORNAT F3 CALC F4
300 - STAT
DEL
PRGM
250 COS
(
150 8
5
100 2
.
50
0 ml
Vernier
Texas Instruments
TI-84 Silver Edition
CALIBRATION SENSOR 1
ENTER VALUE
MONITOR VOLTAGE, WHEN
?4
STABLE,PRESS [ENTER].
VALUE VOLTAGE LabPro Calculator
POINT 1: 4 1.635
1.635
1.631
1.729
POINT 2: Screen
Step 6: Rinse the electrode with DI water. Next, place the pH Previous Slide
electrode into a buffer that has a pH of 7. Allow the voltage to stabilize to a
reasonably constant value out to the hundredths place past the decimal. Replay
Once this occurs, press the ENTER key. On the next screen, press the 7
Continue
key to input the pH of this buffer into the calculators memory. Press
ENTER to complete the calibrating process.
Power Supply
Quick Setup
Transfer Start/Stop
VERNIER
LabPro
SENSOR
pH
TBLSET F2 FORNAT F3 CALC F4
300 - STAT
DEL
PRGM
250
COS
(
150 8
5
100 2
.
50
0 ml
Vernier
Texas Instruments
TI-84 Silver Edition
CALIBRATION SENSOR
MONITOR VOLTAGE, WHEN
STABLE,PRESS [ENTER].
VALUE VOLTAGE LabPro Calculator
POINT 1: 4 1.635
POINT 2: 7 3.971 Screen
Step 6: Rinse the electrode with DI water. Next, place the pH Previous Slide
electrode into a buffer that has a pH of 7. Allow the voltage to stabilize to a
reasonably constant value out to the hundredths place past the decimal. Replay
Once this occurs, press the ENTER key. On the next screen, press the 7
Continue
key to input the pH of this buffer into the calculators memory. Press
ENTER to complete the calibrating process.
Power Supply
Quick Setup
Transfer Start/Stop
VERNIER
LabPro
SENSOR
pH
TBLSET F2 FORNAT F3 CALC F4
300 - STAT
DEL
PRGM
250
COS
(
150 8
The pH sensor is
5
100 2
.
50
now calibrated!
0 ml
Vernier
Texas Instruments
TI-84 Silver Edition
CALIBRATION
CALIBRATION SENSOR 1
ENTER VALUE
PH
MONITOR VOLTAGE, WHEN
?7
CALIBRATION: LINEAR
STABLE,PRESS [ENTER].
SLOPE
819
INT
VALUE
-21364
VOLTAGE LabPro Calculator
POINT 1: 4 1.635
POINT 2: 7
1:OK
3.971
3.976
3.971
4.452 Screen
2:CALIBRATE NOW
3:MANUAL ENTRY
Previous Slide
Step 7: Press the 1 key [OK] to return to the Settings
menu. Press the 1 key [OK] again to return to the main Replay
menu.
Continue
Power Supply
Quick Setup
Transfer Start/Stop
VERNIER
LabPro
SENSOR
pH
TBLSET F2 FORNAT F3 CALC F4
300 - STAT
DEL
PRGM
250
COS
(
150 8
5
100 2
.
50
0 ml
Vernier
Texas Instruments
TI-84 Silver Edition
CALIBRATION
PH
CALIBRATION: LINEAR
SLOPE INT
819 -21364
1:OK
2:CALIBRATE NOW
3:MANUAL ENTRY
Previous Slide
Step 7: Press the 1 key [OK] to return to the Settings
menu. Press the 1 key [OK] again to return to the main Replay
menu.
Continue
Power Supply
Quick Setup
Transfer Start/Stop
VERNIER
LabPro
SENSOR
pH
TBLSET F2 FORNAT F3 CALC F4
300 - STAT
DEL
PRGM
250
COS
(
150 8
5
100 2
.
50
0 ml
Vernier
Texas Instruments
TI-84 Silver Edition
CH 1: PH CALIBRATION
CH PH 7
CH 2:
PH 3:
CH
CALIBRATION: LINEAR
CH 4:
INT
SLOPE TIME GRAPH-180
Mode:
DIG1:
819
DIG2: -21364
1:MODE: TIME GRAPH-900
SETUP
1:OK
4: ANALYZE pH reading for a
2: START NOW 5: TOOLS
1:OK
2:CALIBRATE 3:ZERO
3:MANUAL ENTRY 6: QUIT
3: GRAPH
2:CALIBRATE 4:SAVE/LOAD solution
Previous Slide
Calibrating The pH Sensor: Review of Steps 5-7 Replay
Continue
Texas Instruments Texas Instruments Texas Instruments
TI-84 Silver Edition TI-84 Silver Edition TI-84 Silver Edition
CALIBRATION CALIBRATION SENSOR 1
PH
ENTER VALUE
MONITOR VOLTAGE, WHEN
CALIBRATION: LINEAR STABLE,PRESS [ENTER]. ?4
SLOPE INT VALUE VOLTAGE
0 4 POINT 1: 2.627
POINT 2:
1:OK
2:CALIBRATE NOW
3:MANUAL ENTRY
(1) Press 2: CALIBRATE NOW (3) Press 4 Key then
(2) Press ENTER key
the ENTER Key
Texas Instruments Texas Instruments Texas Instruments
TI-84 Silver Edition TI-84 Silver Edition TI-84 Silver Edition
CALIBRATION SENSOR 2 CALIBRATION
ENTER VALUE
MONITOR VOLTAGE, WHEN PH
STABLE,PRESS [ENTER].
?7 CALIBRATION: LINEAR
VALUE VOLTAGE SLOPE INT
POINT 1: 4 1.635 819 -21364
POINT 2: 3.458
1:OK
2:CALIBRATE NOW
3:MANUAL ENTRY
(4) Press ENTER key (5) Press 7 key then (6) Press 1 twice to return to
the ENTER key default menu screen
Previous Slide
Calibrating The pH Sensor: Review of Steps 5-7 Replay
Continue
Texas Instruments Texas Instruments Texas Instruments
TI-84 Silver Edition TI-84 Silver Edition TI-84 Silver Edition
CALIBRATION CALIBRATION SENSOR 1
PH
ENTER VALUE
MONITOR VOLTAGE, WHEN
CALIBRATION: LINEAR STABLE,PRESS [ENTER]. ?4
SLOPE INT VALUE VOLTAGE
0 4 POINT 1: 2.627
POINT 2:
1:OK
2:CALIBRATE NOW
3:MANUAL ENTRY
(1) Press 2: CALIBRATE NOW (3) Press 4 Key then
(2) Press ENTER key
the ENTER Key
Texas Instruments Texas Instruments Texas Instruments
TI-84 Silver Edition TI-84 Silver Edition TI-84 Silver Edition
CALIBRATION SENSOR 2 CALIBRATION
ENTER VALUE
MONITOR VOLTAGE, WHEN PH
STABLE,PRESS [ENTER].
?7 CALIBRATION: LINEAR
VALUE VOLTAGE SLOPE INT
POINT 1: 4 1.635 819 -21364
POINT 2: 3.458
1:OK
2:CALIBRATE NOW
3:MANUAL ENTRY
(4) Press ENTER key (5) Press 7 key then (6) Press 1 twice to return to
the ENTER key default menu screen
End of Tutorial 4
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