DNA SEQUENCE SUBMISSION FORM – INTERNAL
Phone: (905) 525-9140 ext. 27048 Fax: (905) 526-1427 E-mail: firstname.lastname@example.org http:// www.science.mcmaster.ca/mobix/
SUPERVISOR: TELEPHONE #:
PI SIGNATURE: ROOM #:
EDITING REQUEST** WITH ($15.00) WITHOUT($7.50)
RESULTS E-MAILED TEXT FILE ABI FILE
SPECIAL CONSIDERATION: 1) high GC content (add dmso) YES
2) difficult template (hairpin protocol*) YES * ($9.00 per rxn, 1 to 2 additional days)
# Name of DNA Name of PRIMER # Name of DNA Name of PRIMER
*MAX 8 CHARACTERS *MAX 8 CHARACTERS *MAX 8 CHARACTERS *MAX 8 CHARACTERS
SAMPLE AND PRIMER PREPARATION - (sample & primer should be suspended in Water). We require 5μl of sample and 5μl primer per reaction.
**Editing is the correction of miscalled bases. These problems generally occur at the beginning and end of the sequence, but can occur anywhere.
Note – you require the ABI file and the correct computer software to do your own editing.
Sample Concentration – because sequencing is unidirectional the number of copies of your template in the sample becomes very important, thus we require minimum
concentration of DNA to perform the reaction. Too much DNA can result in a short read or a smear. The sample concentrations listed below take into account the
TOTAL amount of DNA in the sample (i.e. plasmid + insert) and are concentrations calculated to encourage success.
Please indicate your DNA concentration required
Total Sample Size ds DNA <10 kb 200 ng/μl approx. size
ds DNA 10kb-200kb 0.25-1ug/μl approx. size
PCR 100-500 bp 5-20ng/μl approx. size
PCR 500-2000 bp 20-40ng/μl approx. size
PCR >2000 bp 100-200ng/μl approx. size
Primer Concentration: 1 picomole/μl = [1 μM]
SAMPLE STORAGE: we keep the DNA samples and primers for one month after they have been processed.
Mobix Lab, LSB-B123, McMaster University, 1280 Main Street. W., Hamilton, ON, L8S 4K1