Microbiology 409 Laboratory Syllabus:

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					Microbiology 409 Laboratory Syllabus:

Lab no.      Date           Exercise #       Lab title                            Page #
#1           Jan. 11 (T)    1                Brightfield microscopy               3-11
                                             Observing bacteria (intro)           47, 101-103
#2           Jan. 13 (Th) 6                  Ubiquity of Bacteria                 47-51
                                             Evaluation of Alcohol                211-212
                             8               Aseptic technique                    63-72
                             12              Negative Staining                    93-94
#3           Jan. 18 (T)     6               Ubiquity of Bacteria
                                             -Evaluation of results
                                             Aseptic technique
                                             -Evaluation of results
                                             Evaluation of Alcohol
                                             -Evaluation of Results
      Hand in after class: exercise 6, 8, and 32 (pg 49-51, 71-72 and 2213-214)
#4           Jan. 20 (Th) 10                 Smear Preparation                    87-90
                             11              Simple Staining                      91
#5                           QUIZ 1 (covering exercise 1, 6, 8, 10-11, 32)
             Jan. 25 (T)     13              Capsular Staining                    95-96
                             14              Gram Staining                        101-104
      Hand in after class: exercise 10-13 (pg 97-99)
#6           Jan. 27 (Th) 14                 Gram Staining continued              101-104
#7           Feb. 1 (T)      --              NO LAB- EXAM 1 in LECTURE
#8           Feb. 3 (Th) 15                  Spore Staining                       105-107
                             16              Acid Fast Staining                   109-110
      Hand in after class: exercise 14-16 (pg 111-114)
#9           Feb. 8 (T)      QUIZ 2 (covering exercises 14-16)
                             9               Pure culture                         73-80
                             17               Motility                            115-118
#10          Feb. 10 (Th) 9                  Pure culture
                                             -Evaluation of results
                                             -Subculturing
                                             Motility
                                             -Evaluation of results
      Hand in after class: exercise 9 (pg 81-83) and 17 (119-120)
#11          Feb. 15 (T) QUIZ 3 covering exercises 9 and 17)
                             20              Enumeration of Bacteria              139-145
#12          Feb. 17 (Th) 20                 Enumeration of Bacteria
      Hand in after class: exercise 4 (pg 29) and exercise 20 (pg 147-149)
#13          Feb. 22 (T) QUIZ 4 (covering exercise 20)
                             36              Study of Unknown                     239-242
                                             -Nutrient Broth Culture
Laboratory Syllabus Continued:
Week         Date            Exercise #     Lab title                            Page #
#13          Feb. 22 (T) 36 (continued) Study of Unknown
                                                 Gram Stain
                                                 Microscopic Measurement
                                                 Motility
#14          Feb. 24 (Th) 36                Study of Unknown
                                            -Motility evaluation
                                            -Interpretation of Results
                                            -Nutrient Agar Slants
                                            -Spore and Capsular Stain
      Hand in after class: exercise 36 (pg 243)
#15          Mar. 1 (T)      --             Environmental Sampling
#16          Mar. 3 (T)      --             NO LAB- EXAM 2 in LECTURE
#17          Mar. 15 (T) QUIZ 5 (covering exercise 20 and 36)
                             37             Cultural Characteristics             245-248
                             38             Physiological Characteristics:       249-259
#18          Mar. 17 (Th) 37                Cultural Characteristics
                                            -Evaluation of Cultures
                                            Physiological Characteristics
                                            -Evaluation of Tests
#19          Mar. 22 (T) QUIZ 6 (covering exercise 37 and 38)
                                            Physiological Characteristics:       261-265
                                            Hydrolytic and Degradative Rxn
                             40             Physiological Characteristics:       267-270
                                            Multiple Test Media
                             43             Enterobacteriaceae Identification:   291-295
                                            The Enterotube II System
#20          Mar. 24 (Th) 39                Physiological Characteristics:       261-265
                                            Hydrolytic and Degradative Rxn
                                            -Evaluation of Tests
                             40             Physiological Characteristics:       267-270
                                            Multiple Test Media
                                            -Evaluation of Tests
                             43             Enterobacteriaceae Identification:   291-295
                                            The Enterotube II System
                                            -Evaluation of Tests
                             41             Use of Bergey’s Manual               257-279
      Hand in after class: exercise 38-40 (pg 271-274)
      What bacteria is your unknown? (worth 10 points)
#21          Mar. 29 (T) QUIZ 9 (covering exercise 39-41 and 43)
                             42             Enterobacteriaceae Identification:   283-289
                                            The API E20 Strip
#22          Mar. 31 (Th) 42                Enterobacteriaceae Identification:   283-289
                                            The API E20 Strip
                                            -Identification of Unknown
      Hand in after class: exercise 42 (pg288-289)
Laboratory Syllabus Continued:

Week          Date          Exercise #     Lab title                                 Page #
#23           Apr. 5 (T)                   NO LAB- EXAM 3 in LECTURE
#24           Apr. 7 (Th)   46             Bacterial Counts on Foods                 317-320
                            20             Enumeration of Bacteria                   139-145
       Bring in food sample!
#25           Apr. 12 (T) 46               Bacterial Counts on Foods
                                           -Evaluation of Results
                                               Gram Stain
                                               Simple Stain
                                               Pure Culture Technique: Streak Plate for Isolation
              Apr. 14 (Th) 47              Bacterial Examination of Water            321-330
       Bring in water sample!
#26           Apr. 19 (T) QUIZ 10 (covering exercise 41, 42 and 46-47)
                              47              Bacterial Examination of Water         321-330
                                              -Evaluation of Results
                              33              Antibiotic Sensitivity Testing         215-228
                              34              Evaluation of Antiseptics              229-236
       Bring in possible antibiotic/antiseptic!
#27           Apr. 21 (Th) 33                 Antibiotic Sensitivity Testing         215-228
                                              -Evaluation of Results
                              34              Evaluation of Antiseptics              229-236
                                              -Evaluation of Results
       Hand in after class: exercise 33 and 34 (pg 221-222 and pg227-228)
#28           Apr. 26 (T) Lab- Practicum EXAM
#29           Apr. 28 (Th) NO LAB—Lecture Study Day---

				
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