Slide 1 by 242e0MF

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									                           Question
• What signaling pathways work during pheromone response in yeast.
• 200 genes induced by alpha factor and
  200 genes repressed
• Gene repression required Far1p (2E)
• Entire response is from pathway activation
  of Ste12p
• 120 min time point was to look at
  schmooing genes – MAPKs associated
  with PKC pathway – MPK1, MLP1
• bni1 cells initatied G1 arrest but didn’t
  show increased transcription of MPK1 and
  other late-induced genes
• far1, kss1, or tec1 cells in the presence of
  pheromone show invasive, filamentous
  growth
        Clustered
       experiments

Conclusions: can be used to trace
signaling mechanisms and the
circuits that underlie complex
biological processes
                  Goal:
• Different approaches to use array data to
  predict protein function
• Use different clustering algorithms and
  different parameters to obtain many
  clusters – basically bootstrapping
                  results
• Of the 13,862 clusters, 44% were
  significant in one or more categories at
  P<0.01/N
• Identified the most significant annotation
  for each cluster
             assignments
• 179 new genes thought to function in
  carbohydrate metabolism
• 249 in aa metabolism
• 285 RNA processing – (97/37% are
  essential), 47% conserved in humans
  (BLAST E<10-10
Northern blots to test

								
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