Eubacteria Part One by yurtgc548

VIEWS: 38 PAGES: 220

									Domain: Bacteria
Kingdom:
 Eubacteria
What is this?
If this is how many bacteria are on the head
of a pin. Imagine how many are on your
hands right now.
“Imagine how
 much bacteria
 exist in snot!”
Domain Bacteria, (Kingdom Eubacteria)
Not Living
1-5 microns
       N-95 Particulate Respirator
   Filters 95% of particle
    >1 micron in size
   Good for bacteria
   Not good for viruses
   Not good for infected
    patient use!
   Why?
Bacterial Structures
Who was paying attention?
   Prokaryotic (No nucleus) and no internal
    organelles.
   Prokaryotic (No nucleus) and no internal
    organelles.
   Prokaryotic (No nucleus) and no internal
    organelles.
   Prokaryotic (No nucleus) and no internal
    organelles.
   Prokaryotic (No nucleus) and no internal
    organelles.
   Prokaryotic (No nucleus) and no internal
    organelles.
   Prokaryotic (No nucleus) and no internal
    organelles.
   Prokaryotic (No nucleus) and no internal
    organelles.
   Prokaryotic (No nucleus) and no internal
    organelles.
   Prokaryotic (No nucleus) and no internal
    organelles.
   Prokaryotic (No nucleus) and no internal
    organelles.
   Prokaryotic (No nucleus) and no internal
    organelles.
   Prokaryotic (No nucleus) and no internal
    organelles.
   Prokaryotic (No nucleus) and no internal
    organelles.
   Prokaryotic (No nucleus) and no internal
    organelles.
Which cell has a nucleus (Eukaryotic), and
which is a bacteria (Prokaryotic).
Cell with nucleus   Cell without nucleus
Eukaryotic          Prokaryotic (Bacteria)
   Two types:
       1.) Archaea – old
       2.) Eubacteria -true
   Two types:
       1.) Archaea – Old
       2.) Eubacteria -true
   Two types:
       1.) Kingdom Archaeabacteria – Old
       2.) Kingdom Eubacteria -True
   Kingdom Archaebacteria:
      Kingdom Archaebacteria
 Single celled, prokaryotic
 DNA is similar to Eukaryotes
 Pseudopeptidoglycan cell wall
 Live in harsh conditions
Kingdom Archaebacteria
             Don’t need sunlight
             Use chemosynthesis

             High in sulfides

             High in methane
      Kingdom Archaebacteria
 High temperatures
 Hot springs in
  Yellowstone
  National Park
 First discovered
   These bacteria are found in extreme
    places.
     Many  scientists think this type of life may be
      found in the harsh environments on other
      planets.
   Kingdom Eubacteria: True bacteria, gets energy
    from food or sun.
• Microscopic picture of bacteria hiding
  inside a human lung.
• Bacteria living off tissue
• Bacteria can be autotrophs or hetertrophs.




• Those that are classified as autotrophs are
  either photosynthetic, obtaining energy
  from sunlight or chemosynthetic, breaking
  down inorganic substances for energy .
• Heterotrophs derive
  energy from breaking
  down complex
  organic compounds in
  the environment.
  This includes
  saprobes, bacteria
  that feed on
  decaying material
  and organic wastes,
  as well as those that
  live as parasites,
  absorbing nutrients
  from living organisms.
   Types of Bacteria
   Types of Bacteria
     Sphere   (Round) Shaped: Cocci
   Rod shaped: Bacilli
   Spiral shaped: Spirilli
   Mycoplasma bacteria: Smallest known life form.


Mycoplasma hominis        Ureaplasma urealyticum
    Fried Egg Colonies       Crunchy Asteroid Colonies
• Mycoplasma bacteria does not have a
  cell wall.
  – Causes many diseases including
    pneumonia.
• Video: Bacteria Classification.
• Quiz 1-10 Cocci, Bacilli, Spirilla,
  Mycoplasma.
1
2
3
4
5
6
7
8
9
10
• Bonus: What is my full name?
• Answers to the Quiz.




                         Happy  80th
                         Birthday!
1
1   Baccilli
2
2   Spirilli
3
3   Cocci
4
4   Baccilli
5
5   Mycoplasma
6
6   Baccilli
7
7   Cocci
8
8   Spirilli
9
9   Spirilli
10
10   Cocci
• Bonus: What is my full name?
• Bonus: Draco Malfoy
  – From the Harry Potter series.
• How Bacteria are organized also tells us a
  lot about them.
• Naming bacteria basics.
  – Use shape and how they organize themselves
    to help name them.
• Naming bacteria basics.
  – Use shape and how they organize themselves
    to help name them.
• Naming bacteria basics.
  – Use shape and how they organize themselves
    to help name them.
• Naming bacteria basics.
  – Use shape and how they organize themselves
    to help name them.
• Naming bacteria basics.
  – Use shape and how they organize themselves
    to help name them.
• Naming bacteria basics.
  – Use shape and how they organize themselves
    to help name them.
• Naming bacteria basics.
  – Use shape and how they organize themselves
    to help name them.
   Diplo = Pair
   Tetrad = Groups of four.
• Tetrad: Bacteria that fail to separate after
  they divide, but instead remain in groups
  of four forming squares.




       Micrococcus   sp.
   Sarcinae = Groups of Eight.
• Sarcinae: Tetrad bacteria that fail to
  separate after they divide, but instead
  remain in groups of eight forming cubes.

      Plant   pathogen in water and dirt
   Staphylo = Cluster
   Strepto = Chain
   Gram staining: Technique used to identify
    bacteria.
     -Heat fix bacteria to slide
     -Crystal violet (20 secs)
     -Rinse w/water
     -Iodine (20 secs)
     -Decolorize with acetone
     -Rinse w/water
     -Safranin (20 secs)
     -Rinse w/water
     -View under oil immersion lens
Acetone  removes
the outer layer of
gram negative
bacteria
   Gram Positive = Dark Purple
• Purple starts with a “P” and so does the
  word positive. Purple = Gram positive.
   Pink and Red: Gram Negative
• A pink slip can often mean negative
  things. Pink = Gram negative.
• Which picture is gram positive bacteria,
  and which is gram negative bacteria?
• Gram Positive   Gram Negative
• Quiz 1-10 Name the type of bacteria, be
  specific so include diplo, tetrad, sarcinae,
  stepto, staphylo, and gram + or – if
  applicable. As well as Cocci, Bacilli, and
  Spirilla. Anything goes.
Warm up round before quiz
• One more practice before Quiz Wiz.
  – Which bacteria is the arrow pointing to?
• Answer: Cocci
• Practice before Quiz Wiz.
  – Which bacteria is the arrow pointing to?
• Answer: Spirilli
• Practice before Quiz Wiz.
  – Which bacteria is the arrow pointing to?
• Answer: Bacilli
1
2
3
4
5
6
7
8
9
10
• Bonus – Who am I?   “I pity the
                        fool that
                        doesn’t
                         respect
                       bacteria!”
• Answers to the Quiz 1-10.
1
1Gram +
Staphylococci
2
2   Gram - Bacilli
3
3Gram +
Streptococci
4
4Gram +
Tetracocci
5
5   Gram + Bacilli
6
6Gram -
Diplococci
7
7Gram + Bacilli
(with spores!)
8
8   Baccilli (Gram -)
9
9Staphylococcus
(Gram +)
10
10   Streptobacillus
(Gram   +)
  Streptobacillus
10
moniliformis
(Rat   Bite Fever)
• Bonus – Who am I?   “I pity the
                        fool that
                        doesn’t
                         respect
                       bacteria!”
• Bonus – Mr. T

 “That’s right
  fool, and
  don’t forget
  it!”
Tools of the Trade
Tools of the Trade

      BAP
Tools of the Trade

      BAP
               Choc
     Tools of the Trade
Mac
           BAP
Conkey              Choc
 Gram Positive Cocci/Bacilli
Mac
           BAP
Conkey               Choc
 Gram Positive Cocci/Bacilli
Mac
           BAP
Conkey               Choc
 Gram Positive Cocci/Bacilli
Mac
           BAP
Conkey               Choc
 Gram Negative Diplococci
Mac
          BAP
Conkey             Choc
 Gram Negative Diplococci
Mac
          BAP
Conkey             Choc
 Gram Negative Diplococci
Mac
          BAP
Conkey             Choc
 Gram Negative Diplococci
Mac
          BAP
Conkey             Choc
   Gram Negative Bacilli
Mac
          BAP
Conkey              Choc
   Gram Negative Bacilli
Mac
          BAP
Conkey              Choc
     Gram Negative Bacilli
 Mac
                    BAP
 Conkey                   Choc




Haemophilus   sp
     Gram Negative Bacilli
 Mac
                    BAP
 Conkey                   Choc




Haemophilus   sp
     Gram Negative Bacilli
 Mac
                    BAP
 Conkey                   Choc




Haemophilus   sp
     Gram Negative Bacilli
 Mac
                    BAP
 Conkey                   Choc




Haemophilus   sp
     Gram Negative Bacilli
 Mac
                    BAP
 Conkey                   Choc




Haemophilus   sp
                Gram Positive
   Staphylococcus
    aureus
   S. aureus
   Beta Haemolytic
   Large white colonies
   Catalase +
                Gram Positive
   Streptococcus
    pyogenes
   Beta Strep group A
   Beta Haemolytic
   Pinpoint white
    colonies
   Catalase -
                 Haemolysis
   5% Sheep Blood Agar
   Lyse RBC’s
   Alpha
   Beta
   Gamma
              Lactose Fermenting
   Lactose Fermenters
       Pink on MAC
       E. coli
   Non-Lactose
    Fermenters
       Clear on MAC
       P. aeruginosa
                 Gram Negative
 E.coli
 Flat Lactose
  Fermenter
 Indole +
 Smelly!
               Gram Negative
   K. pneumoniae
   Slimy-capsule
   Lactose Fermenter
   Indole –
   CF Patients
             Gram Negative
 P. aeruginosa
 Shiny Non-Lactose
  Fermenter
 Oxidase +
 Grapey Odor
      Gram Negative Diplococci
 N. gonorrhoeae
 Only on Choc
 Very Stinky!!!!
            Gram Positive Bacilli
   Bacillus anthracis
   Anthrax
   Gram + w/spores
   Double zone of beta
    haemolysis!!
   Didn’t look for until
    after 9/11!
So much much more!
So much much more!
So much much more!
So much much more!

								
To top