Culture collection by ajizai

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									                                                                       Phytoplankton Cultures
                                                                    Contact: Kevin Wyman


     Genus           species          Class           Clone ID          Synonyms

Anabaena        flos-aqua      Cyanophyceae          UTEX 2557
Chaetoceros     dichaeta       Coscinodiscophyceae   CCMP 1751

Chlamydomonas                  Chlorophyceae         CC125

Chlamydomonas                  Chlorophyceae         CC124 mt-

Chlamydomonas                  Chlorophyceae         Spr nit+ mt+           1.4

Chlamydomonas                  Chlorophyceae         Spr nit+ mt+           5.5


Chlamydomonas                  Chlorophyceae         Spr nit+ mt+           6.4

Chlamydomonas                  Chlorophyceae         DCMU

Chlamydomonas                  Chlorophyceae         3.12.8

Chlamydomonas                  Chlorophyceae         Vg126

Chlamydomonas                  Chlorophyceae         CC2916               I39 mt -

Chlamydomonas                  Chlorophyceae         CC2686              st-1-1 mt-

Chlamydomonas                  Chlorophyceae         CC2687                st-2-1
Corethron       criophilum     Coscinodiscophyceae   CCMP 1754
Coscinodiscus   cf_concinnus   Coscinodiscophyceae   CCMP 1544

Cryptomonas     erosa

Cryptomonas     ozolini

Cryptomonas     reflexa

Dunaliella      tertiolecta    Chlorophyceae         CCMP 1320             DUN



Dunaliella      tertiolecta    Chlorophyceae                              Old DUN

Emiliana        huxleyi        Prymnesiophyceae      CCMP 374
Eucampia        antarctica     Coscinodiscophyceae   CCMP 1452

Isochrysis      galbana        Prymnesiophyceae      CCMP 1323              ISO
Phaeodactylum   tricornutum       Bacillariophyceae     CCMP 1327   PHAEO

Symbiodinium    microadriaticum   Dinophyceae           CCMP 1633

Symbiodinium    microadriaticum   Dinophyceae
Synechococcus   leopoliensis      Cyanophyceae          UTEX 625

Synechocystis   sp.               Cyanophyceae          PCC 6803

Synechocystis   sp.               Cyanophyceae


Synechocystis   sp.               Cyanophyceae

Thalassiosira   pseudonana        Coscinodiscophyceae   CCMP 1335    3H
Thalassiosira   tumida            Coscinodiscophyceae   CCMP 1470

Thalassiosira   weissflogii       Coscinodiscophyceae   CCMP 1336   ACTIN



Trichodesmium   erythreum         Cyanophyceae          IMS101
ytoplankton Cultures
Kevin Wyman


                  Location                   Media                         Comments
         Rm 324 EGC Reach-in; 26 C
         12/12 L/D                     Bold -N
         REVCO 4 deg                   f/2
         Rm 324 EGC Reach-in; 26 C
         12/12 L/D                     TAP              Wild type obtained from Boynton lab
         Rm 324 EGC Reach-in; 26 C
         12/12 L/D                     TAP              Wild type obtained from Boynton lab
         Rm 324 EGC Reach-in; 26 C                      A251L transformant with SspI RFLP and A251L in
         12/12 L/D                     TAP              psbA, spr marker in 16S rRNA.
         Rm 324 EGC Reach-in; 26 C
         12/12 L/D                     TAP

         Rm 324 EGC Reach-in; 26 C                      wildtype transfomant with SspI RFLP and wildtype
         12/12 L/D                     TAP              A251 in psbA, spr marker in 16S rRNA.
         Rm 324 EGC Reach-in; 26 C
         12/12 L/D                     TAP              ?
         Rm 324 EGC Reach-in; 26 C
         12/12 L/D                     TAP              ?
         Rm 324 EGC Reach-in; 26 C
         12/12 L/D                     TAP + arginine   ?
         Rm 324 EGC Reach-in; 26 C
         12/12 L/D                     TAP              Startch mutants from Boynton lab
         Rm 324 EGC Reach-in; 26 C
         12/12 L/D                     TAP              Startch mutants from Boynton lab
         Rm 324 EGC Reach-in; 26 C
         12/12 L/D                     TAP              Startch mutants from Boynton lab
         REVCO 4 deg                   f/2
         Rm 325A 16 C 12/12 L/D        f/2
                                                        isolated from Lake Michigan courtesy of Gary
         Schofield Lab                 MBL              Fahnenstiel
                                                        isolated from Lake Michigan courtesy of Gary
         Schofield Lab                 MBL              Fahnenstiel
                                                        isolated from Lake Michigan courtesy of Gary
         Schofield Lab               MBL                Fahnenstiel
         Rm 325A 16 C 12/12 L/D also
         window rm 304               f/2 -Si
                                                        This is "old" culture that has been in Falko's lab
                                                        for long time, but may have different
         Rm 325A 16 C 12/12 L/D also                    characteristics from newer CCMP 1320,
         window rm 305                 f/2 -Si          especially in Caspase activity
         Rm 325A 16 C 12/12 L/D also
         window rm 303                 f/2 -Si
         REVCO 4 deg                   f/2
         Rm 325A 16 C 12/12 L/D also
         window rm 302                 f/2 -Si
Rm 325A 16 C 12/12 L/D also
window rm 308               f/2
Rm 324 EGC Reach-in; 26 C
12/12 L/D                   f/2 - Si      from Aiptasia
Rm 324 EGC Reach-in; 26 C
12/12 L/D                   f/2 - Si      from Tridacna gigas courtesy of ?
                            Allen
Rm 324 EGC Reach-in; 26 C
12/12 L/D                   BG11
Rm 324 EGC Reach-in; 26 C                 (add glucose to media, chloramphenicol cassette)
12/12 L/D                   BG11+         LOW LIGHT (5 mE m-2 s-1)

Rm 324 EGC Reach-in; 26 C                 D rpaC deletion mutant from Conrad Mullineax,
12/12 L/D                     BG11        London autotrophicstrain, kanamycin cassette
Rm 325A 16 C 12/12 L/D also
window rm 306                 f/2
REVCO 4 deg                   f/2
Rm 325A 16 C 12/12 L/D also
window rm 307                 f/2 ; ASW
                                          We have two cultures; one from John Waterbury
                                          is axenic; the other from SUNY Stony Brook.
Rm 324 EGC Reach-in; 26 C                 Both are the same clone originally isolated by
12/12 L/D                 Tricho          Hans Perl

								
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