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					                                         Workshop

Understanding the genetic and molecular basis or quality and production traits
             in grapevine: From traits to genes and backwards
                  Logroño, La Rioja (Spain), May 6-8 th, 2007


       The availability of the whole grapevine genome sequence in the close future offers
the possibility to start analyzing the relationship between nucleotide sequence variation and
phenotypic variation which is the basis for genetic improvement. Furthermore genome
annotation requires obtaining information on gene biological function through the generation
of reverse genetics strategies.

        The analysis of gene biological function is not easily robotized and requires exploiting
phenotypic variation using genetic strategies to link phenotype to genotype. Different
approaches can use either natural genetic variation present in the species and its close
relatives or develop mutagenesis procedures or efficient gene transfer protocols for gene
functional analyses.

       The development of multiple efficient strategies to establish biological gene function
in grape is a required goal if we want to efficiently exploit the information provided by the
complete sequence of the genome.

We therefore propose to organize a workshop on strategies to establish gene biological
function in grapevine under the auspices of COST Action 858
(http://www.bordeaux.inra.fr/cost858/welcome.html) that would take place in Logroño (La
Rioja), during two days of the second week of May 2007 (6-8 of May).

The aims of the workshop are:

   -   To review the tools and strategies to exploit natural genetic variation in grapevine,
       considering management of genetic resources and databases, development of high
       throughput molecular markers and forward genetics approaches.

   -   To discuss the development and use of experimental systems for grapevine as well
       as the procedures for mutagenesis and mutant analyses.

   -   To review gene transfer procedures and derived reverse genetic strategies for
       functional analyses (Transposon tagging, RNAi, VIGS, activation tagging, etc).

Organizing Committee

José M. Martínez Zapater (Madrid, Spain)
Hipolito Medrano (Palma de Mallorca, Spain)
Serge Delrot (Bordeaux, France)
Goetz Reustle (Germany)
Didier Merdinoglu (Colmar, France)
Lucia Martinelli (San Michele Al’Adige, Italy)
Laurent Torregrosa (Montpellier, France)
Eva Zyprian (Geiwelherhof, Germany)
Stella Grando (San Michele Al’Adige, Italy)
Javier Ibañez (Alcalá de Henares, Spain)
                                  Provisional Programme

                                       Sunday May 6th
20:00 Welcome cocktail

                                      Monday, May 7th
9:30-10:00 Opening session

10:00-11:30 Genetic resources and tools for forward genetic analyses in grapevine I
Topics considered: Genotypic and phenotypic evaluation of genetic resources (Vitis species
and Vitis vinifera ssp, accessions). Construction and exploitation of core collections.
Proposed invited talks:

- Patrice This (Genetic resources)
- Javier Ibañez (Table grape collections)
- Selected talks from communications

11:30-12:00 Coffee break

12:00-14:00 Genetic resources and tools for forward genetic analyses in grapevine II
Topics considered: Clones and somatic variants. High throughput Molecular markers (SSRs
and SNPs)
Proposed invited talks:

- Diego Lijavetzky (SNP detection and application)
- Charles Romieu (Somatic variation)
- Selected talks from communications

14:00 Lunch

16:00-18:00 Forward genetic analyses of natural variation
Topics considered: Quantitative genetic analyses of relevant traits. Genetic association
studies
Proposed invited talks:

- Stella Grando (Quantitative genetic analysis of Muscat aroma)
- Eva Zyprian (Quantitative genetic analysis of pathogen resistance)
- Nathalie Ollat/Stephane Decroocq (QTL analysis of water stress resistance)
- Loic Le Cunff (Genetic association studies in Grapevine)
- Selected talks from communications

18:00 Poster session

20:00 Wine tasting and meeting dinner
                                       Tuesday May 8th
9:00-11:00 Reverse genetics approaches and tools I
Topics: Grapevine genetic systems, Mutagenesis protocols and mutant collections, TILLING
in grapevine?, Genetic transformation strategies, Transposable elements, VIGS, Transgenic
collections (Trans activation, T-DNA lines, RNAi, etc)
Proposed invited talks:

- Carlos Alonso-Blanco (Arabidopsis Tiller)
- Mark Thomas
- Didier Merdinoglu/ Frederique Pelsy
- Selected talks from communications

11:00-11:30 Coffee break

11:30-13:00 Reverse genetics approaches and tools II
- Lucia Martinelli (grape transformation and the GMO issue)
- Bruno Mezzetti (Genetic tranformation via organogenesis)
- Selected talks from communications

13:00-14:00 Round table discussion
Possible topics for discussion

Genetic collections and core collections management and conservation
Genetic systems
Mutagenesis and TILLING
Transgenic collections
Reverse genetics

14:00 Lunch and farewell

15:30- 19:00
Travel to the town of Briones (40 km) and visit to the Museum of Wine Culture for
interested participants.

				
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