# Prediction by k966Xd

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```									   Climate change wet-lab: Effects of temperature on budworm development

Predictions
How do you predict that a 4°C increase in temperature will affect the rate of
development for budworm larvae? Explain your reasoning.

Methods
1. Turn on two incubators, and set one to 24°C and one to 28°C.
2. Wait for the incubators to reach 24°C and 28°C respectively. Use a thermometer
to check.
3. Obtain two containers of budworm diet and two budworm larvae.
4. Carefully place one budworm in each container of diet, and close the lids.
5. Write your initials and class period on each lid so that you can find your
budworms again. Label one lid 24°C and one lid 28°C.
6. Place the container labeled 24°C in the 24°C incubator. Place the container
labeled 28°C in the 28°C incubator.
7. Record the date when the budworm larvae were placed in the incubators.

Date budworm larvae were placed in incubators: _________

8. Check your two budworms once every day to see whether or not they have
developed into pupae. (Budworm larvae are caterpillars, while budworm pupae
are cocoons.)
9. As soon as you notice that one of your budworms has developed into a pupa,
carefully record the date and the temperature at which the budworm was growing.
Continue to monitor the other budworm until it develops into a pupa. At that
time, record the date and the temperature at which was growing.

Pupation date for your budworm reared at 24°C: _________

Pupation date for your budworm reared at 28°C: _________

Dr. Christine Muth, Dr. Amy Sheck                          NC School of Science & Math
Data analysis
The average age at pupation for budworms reared at 24ºC was 23.9 days.
The average age at pupation for budworms reared at 28ºC was 21.7 days.

Make a bar graph to show these results. Think carefully about which variable to put
on the x axis (the independent variable) and which variable to put on the y axis (the
dependent variable). Be sure to label your axes and include units of measure.

Dr. Christine Muth, Dr. Amy Sheck                          NC School of Science & Math
Discussion questions
1. Did temperature affect the age at which budworms pupated? If so, at what
temperature did the budworms pupate most quickly?

2. How do you think budworm development will change in the future with global
warming?

3. Budworms feed on tobacco plants. Budworms are preyed upon by bluebirds,
which are in turn preyed upon by hawks. How do you think this change in
budworm development rate would affect these other populations?

Population     Potential effects due to changes in budworm development rate
Tobacco

Bluebird

Hawk

4. How do you think your predictions about effects of budworms on tobacco
populations would impact tobacco farmers and the NC economy?

Dr. Christine Muth, Dr. Amy Sheck                        NC School of Science & Math

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