Poster WheatCAP PAG 2009

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					                                                                                Soria M., Sherman J., Anderson J., Baenziger P., Bai G., Berzonsky B., Brown-Guedira G.,
                                                                                Campbell K., Carver B., Chao S., Fritz A., Griffey C., Haley S., Johnson J., Kianian S.,
                                                                                Kidwell K., Matthews D., Mergoum M., Ohm H., Peterson J., Riera Lizarazu O., Rudd J.,
                                                                                Talbert L., Sorrells M., Souza E., Zhang W., Zemetra R, and Dubcovsky J.,
                                                                                Please, find the complete list of affiliations in our web site.: http://maswheat.ucdavis.edu




          The WheatCAP project delivers genomic resources for improved wheat breeding
Introduction                                                                  Research                                                                                             Trait                                     Genes, QTLs

                                                                                                                                                                                   Fungal disease resistance (68.6%)

 In 2005, USDA-CSREES-NRI awarded a grant to the                               Breeders design their breeding plans                          MAS datapoints by year
                                                                                                                                                                                       Leaf rust resistance                  Lr19, Lr21, Lr34, Lr35, Yr17/Lr37/Sr38, Lr39,
                                                                                                                                                                                                                             Lr46, Lr47 and Lr52
 WheatCAP consortium to develop new genomic tools and                          using MAS strategies and according to            200,000
                                                                                                                                                                                       Fusarium head blight                  QTLs on chromosome arms 2DL, 3AS, 3BS,
 knowledge to improve and accelerate wheat breeding. Breeders                  their regional needs DNA or leaf                                                                        resistance                            5AS and Qfhs.pur-7EL
 from the major wheat-growing states, the four USDA-ARS                        samples are submitted to the                     150,000
                                                                                                                                                                                       Stripe rust resistance
                                                                                                                                                                                                                             Yr5, Yr15, Yr30, Yr36, Yr39, Q.htap-6BS and
                                                                                                                                                                                                                             other sources of high temperature adult plant
 small grains genotyping centers and GrainGenes participate in                 designated Genotyping Centers. Since                                                                                                          resistance (HTAP)
 the project. The consortium is applying marker assisted                       the beginning of the project more than           100,000                                                Stem rust resistance                  Sr2, Sr24/Lr24, Sr26 and Sr36
 selection (MAS) strategies to improve many traits, with a focus               357,000 analyses were requested. In                                                                 Quality traits (9.6%)
 on disease and pest resistances (rusts, Hessian fly, powdery                  2008 the sum of datapoints obtained               50,000                                                Gluten strength                       Alleles of GluA1, GluA3, GluB1, GluB3 and
                                                                                                                                                                                                                             GluD1
 mildew leaf and glume blotch) and milling and baking quality                  in-house and though the Genotyping                                                                      Grain protein content                 Gpc-B1
 (grain protein, color and texture and gluten strength).                       Centers exceeded 200,000, more than                   0                                                                                       Grain color alleles linked to Xgwm155,
                                                                                                                                                                                       Grain color
                                                                               double the number originally                               2006            2007        2008                                                   Xgwm4010 and Xgwm4306
 Collaborators are also developing longer term public resources                projected.                                                                                                                                    Clark's Cream 3AS soft allele, PinAm and PinA-
                                                                                                                            Number of datapoints breeders                              Grain texture
 by mapping traits in 19 populations, which are being                                                                       requested to their genotyping centers
                                                                                                                                                                                                                             D1
 phenotypically evaluated at an average of seven environments                 During the last three years participants                                                             Insect resistance (8.1%)

 for QTL discovery related to complex traits. Single Nucleotide               released more than 30 cultivars and                                                                      Hessian fly resistance                H10, H13, H22, H25, H26 and Hdicoccum
 Polymorphisms (SNPs) were screened in the parent lines and                                                                                                                            Greenbug resistance                   Gb3
 359 SNPs are being incorporated into the genetic maps.                       germplasm lines that involved the use of molecular markers                                               Russian wheat aphid
                                                                              (http://maswheat.ucdavis.edu/Achievements/cultivars2007.htm)                                             resistance                            Dn2 and Dn7
                                                                                                                                                                                   Virus resistance (5.4%)
 The main goals of the WheatCAP project are:                                  Nineteen population are being used to construct genetic maps, with a                                     BYDV resistance                       Bdv2, Bdv3 and ph1B deletion
                                                                              cumulative sum of 1,169,659 datapoints, mainly SSR markers. That is an                                   WSMV resistance                       WSM-R (Agropyron) and Wsm1
                                                                              average of 413 markers per population, 73% more than the initial target.                                 Wheat stem sawfly resistance          Qss.msub-3BL

  Incorporating genomic tools, especially marker assisted                    Every program also selected a number of traits to phenotype and map.                                 Abiotic stress resistance (4.8%)

   selection (MAS), into the breeding process                                 The CA and IN programs completed their maps with the rest due in 2009.                                   Aluminum tolerance                    ALMT1 and AltBH
                                                                              Detailed information about the mapping populations can be found at                                                                             Q.Xcdo064-1B, QTL on 4A and QTL linked to
  Implementing educational activities to train students in the               http://maswheat.ucdavis.edu/Mapping/
                                                                                                                                                                                       Pre-harvest sprouting                 Xgwm136
                                                                                                                                                                                       Drought tolerance                     Rye translocation linked to SCM9
   use of these techniques
  Informing a broader audience of stakeholders and the                       The parental lines have been screened with a set of 359 Single Nucleotide                            Agronomic traits (3.4%)
                                                                              Polimorphism (SNPs) markers. The mapping of these SNPs is in                                             D-Genome specific                     Set of genes specific for D-genome
   general public on the impact of new technologies in the                    progress. (http://probes.pw.usda.gov:8080/snpworld/Search).
                                                                                                                                                                                                                             chromosomes
                                                                                                                                                                                       Plant height                          alleles of Rht-B1 and Rht-D1
   wheat industry.
                                                                                                                                                                                  Traits and genes targeted by the breeding programs.
                                                                                                                                                                                  Section headings show the trait category and the percent of
                                                                                                                                                                                  datapoints allocated to it. The most frequent traits and the
                                                                                                                                                                                  genes or alleles involved are indicated.
Education                                                                       Outreach
 During 2008 4 high-school students, 46 undergraduate and 42                     Activities for growers and other stakeholders include field                                                                     web usage statistics for the WheatCAP site
 graduate students received training on wheat breeding and                       days and demonstration plots, with more than 53 organized in                                                             2000                                                           6000
 MAS in WheatCAP participating labs. Not all of the students                     2008.
 were financially supported by the WheatCAP, but the project
 effectively leveraged matching funds.                                           WheatCAP collaborators also engaged in other types of                                                                    1500
                                                                                                                                                                                                                                                                         4000
                                                                                 activities, including presentations at industry and scientific




                                                                                                                                                                                                                                                                                pageviews
                                                                                                                                                                                                 visits
 Groups of graduate and postdoctoral students attended courses                   meetings, seminars, press releases and radio interviews.                                                                 1000
 at the Summer Institute of Statistical Genetics, the Plant
 Breeding Workshop and the BarleyCAP Association Mapping                         MAS hands-on workshop shorten the distance between the                                                                                                                                  2000

                                                                                 molecular biology lab and the stakeholder. A small group of                                  Monthly visits              500                                           visits
 Course.                                                                                                                                                                        and number                                                              pageviews
                                                                                 growers visit the MAS lab to learn how molecular markers are                                 of pages read
 Experiential “Combine to Kitchen” trips are occurring                           scored and run analyses with their own hands. Five of these                                      since April
                                                                                                                                                                                                             0                                                           0
                                                                                                                                                                                                                 Apr   May   Jun   Jul   Aug     Sep   Oct   Nov   Dec
 regionally. The trips are meant to raise student awareness                      workshops were organized in 2008.                                                                       ’08.                                            Month
 about all the necessary steps in food production as well as
 increasing student appreciation for grower’s and end-user’s                     The outreach activities are planned, organized and carried out
 perspective. With these goals in mind each group designs                        by breeders taking into account their regional needs. Thus,                                 The project’s web site is a powerful tool to extend our
 region specific trips.                                                          allowing a greater flexibility and insuring success of the                                  outreach activities, with visitors from the US, China, India,
                                                                                 WheatCAP mission to inform stakeholders about new                                           Canada and Europe.
                                                                                 technologies in breeding.
                                                                                                     Af                                                                      The site hosts a section of MAS protocols for 38 traits in
                                                  Students touring Horizon                                                                                                   three main categories: disease and pest resistance, quality
                                                  Milling, Minnesota              Af   A hands-on workshop                                                                   improvement and agronomic. An interactive tool for
                                                                                                                                                                             training in glutenin analysis is also on the site.
                                                                                                                                                                             The education section contains 10
                                                                                                                                                                             Flash animations covering issues
                                                                                                                                                                             like an introduction to molecular
                                                                                                                                                                             breeding, laboratory procedures
                                                                                                                                                                             (PCR, DNA extraction, gel
                                                                                                                                                                             electrophoresis), genetic mapping
The CSU                                                                                                                                                                      and MAS procedures. Visitors can
“Combine to                                                                                                                                                                  find MS-PowerPoint presentations
Kitchen” trip                                                                                                                                                                for preparing molecular breeding
                                                                                                               A field day at
                                                                                                               Virginia Tech                                                 classes (CSI Plant Style) and
                                                                                                                                                                             teachers’ material. Also, we made
                                       Visiting the                                                                                                                          available the class notes and data of
                                              Grain
                                        Exchange                                                                                                                             a course on genetic mapping held at
                                                                                                                                                                             UC Davis in 2006.
 Conclusions
The WheatCAP project is beginning its last year. Throughout the              of breeders, and strengthening of communication with                                     This project is supported by the National
previous three, the project met or exceeded objectives. Important            stakeholders. The project has reinforced a network of wheat                              Research Initiative of USDA’s Cooperative
goals accomplished include: release of adapted germplasm with                breeders and stakeholders upon which new collaborations will be
genes targeted to control serious diseases and improve quality,              built to insure the future improvement of wheat. Future efforts in                       State Research, Education and Extension
identification of new genes and markers to be used in breeding               developing long term genetic resources will include the                                  Service, CAP grant number 2006-55606-
programs, contribution to the education of the next generation               confirmation and utilization of QTLs identified in this project.                         16629.

				
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