Tentative Early Schedule

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					              BIO450: Experimental Flow Chart

Solutions and Media                    Sequence Analysis of
                                        Worm HSP genes
  Grow Worms

Isolate Worm DNA                          PCR Primer Design




                       PCR Amplify HSP
                            genes


                      TOPO Clone genes



                      Isolate plasmid DNA


                        Restriction Map


                      Column Purify DNA


                        Sequence Insert


                       Transform HT115
                             cells


                        Induce dsRNA



                        Feed Worms to
                        generate dsRNA



                      Observe Phenotype
                       of RNAi worms

				
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posted:9/16/2012
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