PETER J. HANSEN PROFESSOR, DEPARTMENT OF ANIMAL SCIENCES UNIVERSITY OF FLORIDA GAINESVILLE, FL 32611-0920 PHONE (352) 392-5590 • FAX (352) 392-5595 EMAIL: HANSEN@ANIMAL.UFL.EDU HTTP://WWW.ANIMAL.UFL.EDU/HANSEN PUBLICATIONS: SEARCH PUBMED Education and Training INSTITUTION AND LOCATION DEGREE YEAR(s) FIELD OF STUDY University of Illinois – Urbana-Champaign B.S. 1974 Agricultural Sciences University of Wisconsin - Madison M.S. 1980 Endocr.-Repro. Phys. University of Wisconsin – Madison Ph.D. 1983 Endocr.-Repro. Phys. University of Florida Postdoc 1983-1984 Biochemistry University of Guelph Sabbatical 1993 Embryology Research Interests The overall research goal is to understand determinants of pregnancy success in ruminants. One major area of research is the elucidation of the cellular and molecular processes by which cellular stress disrupts embryonic function and the intercellular defense systems that embryos use to limit these effects. Of special interest is understanding the molecular basis for the thermotolerance that embryos develop as they advance through development. Focus has been on the role of heat shock proteins, antioxidants, and apoptosis in determining resistance of embryos to elevated temperature. Additional studies are being conducted to elucidate the molecular basis for breed differences in thermal resistance (i.e., cells from Brahman more resistant to heat shock than cells from Angus and Holstein). Applied studies that center largely on embryo transfer are also ongoing to develop strategies for overcoming effects of heat stress on fertility. A second area of research concerns the importance of immunological interactions between the conceptus and mother. Much of this work focuses on progesterone since this hormone plays an important role in suppressing uterine immune function during pregnancy. It is believed progesterone acts, at least in part, by inducing secretion of other molecules that inhibit lymphocyte proliferation. In the sheep, the model species for much of the work, this molecule has been identified as a protein called ovine uterine serpin. Research goals are to document the role of ovine uterine serpin in mediating effects of progesterone and determine the specific steps in lymphocyte activation controlled by the protein. Other research is being conducted to determine the actions of various lymphocyte populations on the function of the preimplantation embryo and placenta. Selected Peer Review Publications (in reverse chronological order) Publications selected from 186 publications (45 in last 5 years) Moreira, F., Paula-Lopes, F.F., Hansen, P.J., Badinga, L., and Thatcher, W.W. (2002) Effects of growth hormone and insulin-like growth factor-I on development of in vitro derived bovine embryos. Theriogenology, 57, 897-905. Paula-Lopes, F.F., and Hansen, P.J. (2002) Heat-shock induced apoptosis in preimplantation bovine embryos is a developmentally-regulated phenomenon. Biol. Reprod. 66, 1169-1177. Al-Katanani, Y.M., Rivera, R.M., and Hansen, P.J. (2002) Seasonal variation in development of in vitro produced bovine embryos. Vet. Rec., 150, 486-487. Al-Katanani, Y.M., and Hansen, P.J. (2002) Induced thermotolerance in bovine two-cell embryos and the role of heat shock protein 70 in embryonic development. Mol. Reprod. Dev. 64, 174-180. Paula-Lopes, F.F., and Hansen, P.J. (2002) Apoptosis is an adaptive response in bovine preimplantation embryos that facilitates survival after heat shock. Biochem. Biophys. Res. Commun. 295, 37-42. Block, J., Chase, C.C., Jr., and Hansen, P.J. (2002) Inheritance of resistance of bovine preimplantation embryos to heat shock: relative importance of the maternal vs paternal contribution. Mol. Reprod. Dev.63, 32-37. Krininger, C.E. III, Stephens, S.H., and Hansen, P.J. (2002) Developmental changes in inhibitory effects of arsenic and heat shock on growth of preimplantation bovine embryos. Mol. Reprod. Dev. 63:335-340. Ocon, O.M., and Hansen, P.J. (2003) Disruption of bovine oocytes and preimplantation embryos by urea and acidic pH. J. Dairy Sci. 86, 1194-1200. Block, J., Drost, M., Monson, R.L., Rutledge, J.J., Rivera, R.M., Paula-Lopes, F.F., Ocon, O.M., Krininger, C.E. III, Liu, J., and Hansen, P.J. (2003) Use of insulin-like growth factor-1 during embryo culture and treatment of recipients with GnRH to increase pregnancy rates following the transfer of in vitro produced embryos to heat-stressed, lactating cows. J. Anim. Sci. 81: 1590- 1602. Soto, P., Natzke, R.P., and Hansen, P.J. (2003) Identification of possible mediators of embryonic mortality caused by mastitis: Actions of lipopolysaccharide, prostaglandin F2a, and the nitric oxide generator, sodium nitroprusside dihydrate, on oocyte maturation and embryonic development in cattle. Am. J. Reprod. Immunol. 50, 263-272. Soto, P., Natzke, R.P., and Hansen, P.J. (2003) Actions of tumor necrosis factor-α on oocyte maturation and embryonic development in cattle. Am. J. Reprod. Immunol., 50, 380-388. Rivera, R.M., Kelley, K.L., Erdos, G.W., and Hansen, P.J. (2003) Alterations in ultrastructural morphology of two-cell bovine embryos produced in vitro and in vivo following a physiologically- relevant heat shock. Biol. Reprod. 69, 2068-2077. Rivera, R.M., Kelley, K.L., Erdos, G.W., and Hansen, P.J. (2004) Reorganization of microtubules and microfilaments by thermal stress in two-cell bovine embryos. Biol. Reprod. 70, 1852-1862. Rivera, R.M., Dahlgren, G.M., de Castro e Paula, L.A., Kennedy, R.T., and Hansen, P.J. (2004) Actions of thermal stress in two-cell bovine embryos: oxygen metabolism, glutathione and ATP content, and the time-course of development. Reproduction, 128, 33-42. Jousan, F.D., and Hansen, P.J. (2004) Insulin-like growth factor-I as a survival factor for the bovine preimplantation embryo exposed to heat shock. Biol. Reprod. 71, 1665-1650. Roth, Z., and Hansen, P.J. (2004) Involvement of apoptosis in disruption of oocyte competence by heat shock in cattle. Biol. Reprod. 71, 1898-1906. Roth, Z., and Hansen, P.J. (2004) Sphingosine 1-phosphate protects bovine oocytes from heat shock during maturation. Biol. Reprod. 71, 2072-2078. Roth, Z., and Hansen, P.J. (2005) Disruption of nuclear maturation and rearrangement of cytoskeletal elements in bovine oocytes exposed to heat shock during maturation. Reproduction, 129, 235- 244. Hernández-Cerón, J., Jousan, F.D., Soto, P., and Hansen, P.J. (2005) Timing of inhibitory actions of gossypol on cultured bovine embryos. J. Dairy Sci. 82, 922-928. Franco, M., and Hansen, P.J. (2006) Effects of hyaluronic acid in culture and cytochalasin B treatment before freezing on survival of cryopreserved bovine embryos produced in vitro. In Vitro Cell Dev. Biol. Anim. 42, 40-44. Franco, M., Block, J., Jousan, F.D., de Castro e Paula, L.A., Brad, A., Franco, J.M., Grisel, F., Monson, R.L., Rutledge, J.J., and Hansen, P.J. (2006) Effect of transfer of one or two embryos and administration of gonadotropin releasing hormone on pregnancy rates of heat-stressed dairy cattle . Theriogenology 66, 224-233. Rhodes, M., Brendemuhl, J.H., and Hansen, P.J. (2006) Litter characteristics of gilts artificially inseminated with TFG-β. Am. J. Reprod. Immunol. 56, 153-156. Franco, M., Thompson, P.M., Brad, A.M., and Hansen, P.J. (2006) Effectiveness of administration of gonadotropin-releasing hormone at day 11, 14 or 15 after anticipated ovulation for increasing fertility of lactating dairy cows and non-lactating heifers. Theriogenology 66, 945-954. Tekin, S., Padua, M.B., Brad, A.M., Rhodes, M.L., and Hansen, P.J. (2006) Expression and properties of recombinant ovine uterine serpin, Exp. Biol. Med. 231, 1313-1323. Bilby, T.R., Block, J., do Amaral, B.C., Sa Filho, O., Silvestre, F.T., Hansen, P.J., Staples, C.R., and Thatcher, W.W. (2006) Effects of dietary unsaturated fatty acids on oocyte quality and follicular development in lactating dairy cows in summer. J. Dairy Sci. 89, 3891-3903. Jousan, F.D. and Hansen, P.J. (2007) Insulin-like growth factor-I promotes resistance of bovine preimplantation embryos to heat shock through actions independent of its anti-apoptotic actions requiring PI3K signaling. Mol. Reprod. Dev. 74, 189-196. Research Support 1. USDA National Research Initiative Competitive Grants Program, "Integrating in vitro embryo technologies into the dairy industry" G.E. Seidel, P.J. Hansen, P. Farin and D.L. Hoag, PIs; 449,907 (UF portion=$118,774); 10/2006-9/2009. 2. Binational Agricultural Research and Development Fund, “Embryo transfer as a tool for improving fertility of heat-stressed dairy cattle”; Grant # US-2551-04; P.J. Hansen (PI) and A. Arav, Agricultural Research Organization, Ministry of Agriculture, Israel; 10/04-10/07; $164,000 for US and $166,000 for Israel. 3. USDA-TSTAR, “Use of embryo transfer to improve fertility of heat-stressed dairy cows” 10/04- 10/07; Grant #2004-34135-14715; $149,862.
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