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									                                               PETER J. HANSEN
                                               PROFESSOR, DEPARTMENT OF ANIMAL SCIENCES
                                               UNIVERSITY OF FLORIDA
                                               GAINESVILLE, FL 32611-0920
                                               PHONE (352) 392-5590 • FAX (352) 392-5595
                                               EMAIL: HANSEN@ANIMAL.UFL.EDU
                                               HTTP://WWW.ANIMAL.UFL.EDU/HANSEN
                                               PUBLICATIONS: SEARCH PUBMED



Education and Training
INSTITUTION AND LOCATION                           DEGREE         YEAR(s)         FIELD OF STUDY
University of Illinois – Urbana-Champaign          B.S.           1974            Agricultural Sciences

 University of Wisconsin - Madison                 M.S.           1980            Endocr.-Repro. Phys.

 University of Wisconsin – Madison                 Ph.D.          1983            Endocr.-Repro. Phys.

 University of Florida                             Postdoc        1983-1984       Biochemistry

 University of Guelph                              Sabbatical     1993            Embryology

Research Interests

The overall research goal is to understand determinants of pregnancy success in ruminants. One
major area of research is the elucidation of the cellular and molecular processes by which cellular
stress disrupts embryonic function and the intercellular defense systems that embryos use to limit
these effects. Of special interest is understanding the molecular basis for the thermotolerance that
embryos develop as they advance through development. Focus has been on the role of heat shock
proteins, antioxidants, and apoptosis in determining resistance of embryos to elevated temperature.
Additional studies are being conducted to elucidate the molecular basis for breed differences in
thermal resistance (i.e., cells from Brahman more resistant to heat shock than cells from Angus and
Holstein). Applied studies that center largely on embryo transfer are also ongoing to develop
strategies for overcoming effects of heat stress on fertility.

A second area of research concerns the importance of immunological interactions between the
conceptus and mother. Much of this work focuses on progesterone since this hormone plays an
important role in suppressing uterine immune function during pregnancy. It is believed progesterone
acts, at least in part, by inducing secretion of other molecules that inhibit lymphocyte proliferation. In
the sheep, the model species for much of the work, this molecule has been identified as a protein
called ovine uterine serpin. Research goals are to document the role of ovine uterine serpin in
mediating effects of progesterone and determine the specific steps in lymphocyte activation controlled
by the protein. Other research is being conducted to determine the actions of various lymphocyte
populations on the function of the preimplantation embryo and placenta.
Selected Peer Review Publications (in reverse chronological order)
Publications selected from 186 publications (45 in last 5 years)
Moreira, F., Paula-Lopes, F.F., Hansen, P.J., Badinga, L., and Thatcher, W.W. (2002) Effects of
    growth hormone and insulin-like growth factor-I on development of in vitro derived bovine
    embryos. Theriogenology, 57, 897-905.
Paula-Lopes, F.F., and Hansen, P.J. (2002) Heat-shock induced apoptosis in preimplantation bovine
    embryos is a developmentally-regulated phenomenon. Biol. Reprod. 66, 1169-1177.
Al-Katanani, Y.M., Rivera, R.M., and Hansen, P.J. (2002) Seasonal variation in development of in
     vitro produced bovine embryos. Vet. Rec., 150, 486-487.
Al-Katanani, Y.M., and Hansen, P.J. (2002) Induced thermotolerance in bovine two-cell embryos and
     the role of heat shock protein 70 in embryonic development. Mol. Reprod. Dev. 64, 174-180.
Paula-Lopes, F.F., and Hansen, P.J. (2002) Apoptosis is an adaptive response in bovine
    preimplantation embryos that facilitates survival after heat shock. Biochem. Biophys. Res.
    Commun. 295, 37-42.
Block, J., Chase, C.C., Jr., and Hansen, P.J. (2002) Inheritance of resistance of bovine
     preimplantation embryos to heat shock: relative importance of the maternal vs paternal
     contribution. Mol. Reprod. Dev.63, 32-37.
Krininger, C.E. III, Stephens, S.H., and Hansen, P.J. (2002) Developmental changes in inhibitory
    effects of arsenic and heat shock on growth of preimplantation bovine embryos. Mol. Reprod.
    Dev. 63:335-340.
Ocon, O.M., and Hansen, P.J. (2003) Disruption of bovine oocytes and preimplantation embryos by
   urea and acidic pH. J. Dairy Sci. 86, 1194-1200.
Block, J., Drost, M., Monson, R.L., Rutledge, J.J., Rivera, R.M., Paula-Lopes, F.F., Ocon, O.M.,
    Krininger, C.E. III, Liu, J., and Hansen, P.J. (2003) Use of insulin-like growth factor-1 during
    embryo culture and treatment of recipients with GnRH to increase pregnancy rates following the
    transfer of in vitro produced embryos to heat-stressed, lactating cows. J. Anim. Sci. 81: 1590-
    1602.
Soto, P., Natzke, R.P., and Hansen, P.J. (2003) Identification of possible mediators of embryonic
    mortality caused by mastitis: Actions of lipopolysaccharide, prostaglandin F2a, and the nitric
    oxide generator, sodium nitroprusside dihydrate, on oocyte maturation and embryonic
    development in cattle. Am. J. Reprod. Immunol. 50, 263-272.
Soto, P., Natzke, R.P., and Hansen, P.J. (2003) Actions of tumor necrosis factor-α on oocyte
    maturation and embryonic development in cattle. Am. J. Reprod. Immunol., 50, 380-388.
Rivera, R.M., Kelley, K.L., Erdos, G.W., and Hansen, P.J. (2003) Alterations in ultrastructural
    morphology of two-cell bovine embryos produced in vitro and in vivo following a physiologically-
    relevant heat shock. Biol. Reprod. 69, 2068-2077.
Rivera, R.M., Kelley, K.L., Erdos, G.W., and Hansen, P.J. (2004) Reorganization of microtubules and
    microfilaments by thermal stress in two-cell bovine embryos. Biol. Reprod. 70, 1852-1862.
Rivera, R.M., Dahlgren, G.M., de Castro e Paula, L.A., Kennedy, R.T., and Hansen, P.J. (2004)
    Actions of thermal stress in two-cell bovine embryos: oxygen metabolism, glutathione and ATP
    content, and the time-course of development. Reproduction, 128, 33-42.
Jousan, F.D., and Hansen, P.J. (2004) Insulin-like growth factor-I as a survival factor for the bovine
    preimplantation embryo exposed to heat shock. Biol. Reprod. 71, 1665-1650.
Roth, Z., and Hansen, P.J. (2004) Involvement of apoptosis in disruption of oocyte competence by
    heat shock in cattle. Biol. Reprod. 71, 1898-1906.
Roth, Z., and Hansen, P.J. (2004) Sphingosine 1-phosphate protects bovine oocytes from heat shock
    during maturation. Biol. Reprod. 71, 2072-2078.
Roth, Z., and Hansen, P.J. (2005) Disruption of nuclear maturation and rearrangement of cytoskeletal
    elements in bovine oocytes exposed to heat shock during maturation. Reproduction, 129, 235-
    244.
Hernández-Cerón, J., Jousan, F.D., Soto, P., and Hansen, P.J. (2005) Timing of inhibitory actions of
   gossypol on cultured bovine embryos. J. Dairy Sci. 82, 922-928.
Franco, M., and Hansen, P.J. (2006) Effects of hyaluronic acid in culture and cytochalasin B
    treatment before freezing on survival of cryopreserved bovine embryos produced in vitro. In Vitro
    Cell Dev. Biol. Anim. 42, 40-44.
Franco, M., Block, J., Jousan, F.D., de Castro e Paula, L.A., Brad, A., Franco, J.M., Grisel, F.,
    Monson, R.L., Rutledge, J.J., and Hansen, P.J. (2006) Effect of transfer of one or two embryos
    and administration of gonadotropin releasing hormone on pregnancy rates of heat-stressed dairy
    cattle . Theriogenology 66, 224-233.
Rhodes, M., Brendemuhl, J.H., and Hansen, P.J. (2006) Litter characteristics of gilts artificially
   inseminated with TFG-β. Am. J. Reprod. Immunol. 56, 153-156.
Franco, M., Thompson, P.M., Brad, A.M., and Hansen, P.J. (2006) Effectiveness of administration of
    gonadotropin-releasing hormone at day 11, 14 or 15 after anticipated ovulation for increasing
    fertility of lactating dairy cows and non-lactating heifers. Theriogenology 66, 945-954.
Tekin, S., Padua, M.B., Brad, A.M., Rhodes, M.L., and Hansen, P.J. (2006) Expression and
    properties of recombinant ovine uterine serpin, Exp. Biol. Med. 231, 1313-1323.
Bilby, T.R., Block, J., do Amaral, B.C., Sa Filho, O., Silvestre, F.T., Hansen, P.J., Staples, C.R., and
    Thatcher, W.W. (2006) Effects of dietary unsaturated fatty acids on oocyte quality and follicular
    development in lactating dairy cows in summer. J. Dairy Sci. 89, 3891-3903.
Jousan, F.D. and Hansen, P.J. (2007) Insulin-like growth factor-I promotes resistance of bovine
    preimplantation embryos to heat shock through actions independent of its anti-apoptotic actions
    requiring PI3K signaling. Mol. Reprod. Dev. 74, 189-196.

Research Support
1. USDA National Research Initiative Competitive Grants Program, "Integrating in vitro embryo
technologies into the dairy industry" G.E. Seidel, P.J. Hansen, P. Farin and D.L. Hoag, PIs; 449,907
(UF portion=$118,774); 10/2006-9/2009.

2. Binational Agricultural Research and Development Fund, “Embryo transfer as a tool for improving
fertility of heat-stressed dairy cattle”; Grant # US-2551-04; P.J. Hansen (PI) and A. Arav, Agricultural
Research Organization, Ministry of Agriculture, Israel; 10/04-10/07; $164,000 for US and $166,000 for
Israel.

3. USDA-TSTAR, “Use of embryo transfer to improve fertility of heat-stressed dairy cows” 10/04-
10/07; Grant #2004-34135-14715; $149,862.

								
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