Fish 420 Lab-manual for parasitology by pHIXRdKl


									                              Fish 424 Laboratory

This laboratory consists of two parts. First we will look at slides and preserved
museum specimens. Second, we will examine histology slides that are positive
and negative for M. cerebralis. Be sure to answer all questions for full credit.

A. Slides and preserved Specimens
 Examine the following categories of parasites using the microscope and direct
 visual examination. Draw a picture of one organism from each major taxa.
 Utilize reference books. Please clean the 100 X (oil) objectives and slides with
 ETOH when finished.

I. Protozoa (Draw 1 organism from the list.)
  1. Costia
  2. M. cerebralis
  3. Henneguya
  4. Ichthyophthirius

II. Trematodes
   1. Gyrodactylus

III. Cestodes
   1. Proteocephalus

IV. Nematodes (Draw 1 organism from the list.)
  1. Philonema
  2. Cucullanus
  3. Anisakis

V. Acanthocephala
 1. Acanthocephalus

VI. Hirudinea
 1. Leech

VII.   Crustacea (Draw 1 organism from the list.)
 1.    Lernaea
 2.    Salmincola
 3.    Actheres

VIII. Fungi
 1. Dermocystidium
IX. Examine museum specimens. What are the drawbacks of using preserved

B. Histology slides
   Examine histology slides positive and negative for M. cerebralis. Draw a
   positive slide, indicating the location of M. cerbralis.

C. Questions
   1. Describe the life cycle of Argulus sp. and site the source you used. (Hint:
      You should use one of the texts from class.)

   2. Describe a digenetic trematode that we did not talk about in class. Site the
      source you used.

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