Fish 424 Laboratory Parasitology Introduction This laboratory consists of two parts. First we will look at slides and preserved museum specimens. Second, we will examine histology slides that are positive and negative for M. cerebralis. Be sure to answer all questions for full credit. A. Slides and preserved Specimens Examine the following categories of parasites using the microscope and direct visual examination. Draw a picture of one organism from each major taxa. Utilize reference books. Please clean the 100 X (oil) objectives and slides with ETOH when finished. I. Protozoa (Draw 1 organism from the list.) 1. Costia 2. M. cerebralis 3. Henneguya 4. Ichthyophthirius II. Trematodes 1. Gyrodactylus III. Cestodes 1. Proteocephalus LIGULA IV. Nematodes (Draw 1 organism from the list.) 1. Philonema 2. Cucullanus 3. Anisakis V. Acanthocephala 1. Acanthocephalus VI. Hirudinea 1. Leech VII. Crustacea (Draw 1 organism from the list.) 1. Lernaea 2. Salmincola 3. Actheres VIII. Fungi 1. Dermocystidium IX. Examine museum specimens. What are the drawbacks of using preserved specimens? B. Histology slides Examine histology slides positive and negative for M. cerebralis. Draw a positive slide, indicating the location of M. cerbralis. C. Questions 1. Describe the life cycle of Argulus sp. and site the source you used. (Hint: You should use one of the texts from class.) 2. Describe a digenetic trematode that we did not talk about in class. Site the source you used.
Pages to are hidden for
"Fish 420 Lab-manual for parasitology"Please download to view full document