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					EU-RUSSIA SYMPOSIUM on S&T CO-OPERATION in BIOTECHNOLOGY
                           and
      EU-RUSSIA PARTNERING EVENT in BIOTECHNOLOGY



                      Agrobiotechnology:
            Plant Genomics and Grain Legume Crops.

                        Prof. Noel Ellis,
                      John Innes Centre,
                         Norwich, UK.

         on behalf of the FP6 Grain Legumes Consortium
www.eugrainlegumes.org
Grain Legumes Integrated Project



1. The problem
2. The approach
3. The partnership
4. The outputs
5. Potential interactions
Grain Legumes Integrated Project

legumes as a proportion of arable agriculture:
                               N & S America
       EU
                                    Asia
       Grain Legumes Integrated Project
Plant proteins in the EU:
 % deficit       Production   Use

       76        03/04
       77        02/03
       77        01/02
                                                      pea, faba, bean, lupin
       74        00/01
       68        99/00                                rapeseed & sunflower

                                                      dry forage
       62        90/91
                                                      soybean meal & seed
       78        80/81
                                                      meat and fish meal
       81        73/74
                                                      miscellaneous
                         5          10   20   protein Mt


                                              Source: UNIP
Grain Legumes Integrated Project

The protein (and Nitrogen) economy of the EU
is considerably out of balance.

A substantial increase in legume protein production
in the EU can redress agricultural imbalance and
meet a large extant demand without a major
impact on trade.

To increase legume protein production we need to
address the current constraints on their production
and use.
Grain Legumes Integrated Project



1. The problem
2. The approach
3. The partnership
4. The outputs
5. Potential interactions
                      Grain Legumes Integrated Project
               Objective :                                 Objective :                                   Objective :                                Objective :
                                                                                                                                          To coordinate and integrate
        To define the impact and                        To understand the                     To develop genomic and post-                   grain legume research,
       potential of improved grain                factors affecting grain legume                 genomic tools needed to                      to provide training in
         legumes on optimised                         seed quality and use.                     improve and sustain grain             emerging technological approaches,
      animal feed and human food.                                                            legume seed quality and supply.                 to disseminate results,
                                                                                                                                       and transfer technology to industry



     Module                  Module              Module                  Module               Module                  Module              Module                Module
        1:                     2:                   3:                      4:                   5:                      6:                   7:                   8:
To identify optimal      To use legumes      To investigate          To identify the      To develop new         To develop new         To provide          To disseminate
  parameters for       to develop healthy   Variation in grain      factors affecting    genetic & genomic      bioinformatic tools   coordination &         knowledge &
 legumes in feed        and sustainable      legume seed              grain legume           tools for          needed to improve        training in      transfer technology
    quality and            Agriculture        Composition           seed composition       grain legume            grain legume        grain legume
      safety                                                                               improvement              seed quality          research

      WP1.1                     WP2.1            WP3.1                   WP4.1                WP5.1                    WP6.1              WP7.1                 WP8.1
Grain Legumes in             Lower Input       Systems               Environmental
                               Farming       approaches to          Effects on Seed         Sequencing             Bioinformatics      Coordination          Dissemination
      Feed
                                            seed composition             Quality

      WP1.2                  WP2.2                WP3.2                 WP4.2                 WP5.2                                       WP7.2                 WP8.2
Feed Processing &         Economic &        Novel approaches       Pathogen Effects
 Nutritional value       environmental        to alter seed                                Mutagenesis                                   Training               Transfer
                                                                    on Seed Quality
                            analysis           composition

                                                                         WP4.3                WP5.3
                                                                   Plant Architecture       Expression
                                                                                             Profiling

                                                                         WP4.4                WP5.4
                                                                   Carbon/nitrogen        Crop & Compar.
                                                                   allocation & seed
                                                                         quality             Genomics


    Agronomy &                                                                          Genetics & genomic
                                                 Trait biology                                                                              Integration
      end use                                                                                  tools
   Grain Legumes Integrated Project
    Distribution of funding 14.4 M€ over 48 months

                                                     WP1.1
                                                     WP1.2
                                                     WP2.1
                                                     WP2.2
                                                     WP3.1
                                                     WP3.2
                                                     WP4.1
                                                     WP4.2
                                                     WP4.3
                                                     WP4.4
                                                     WP5.1
                                                     WP5.2
                                                     WP5.3
                                                     WP5.4

 Genetics                                  Trait     WP6.1
                                                     WP7.1
& genomics                                biology    WP7.2
                                                     WP8.1
                                                     WP8.2
                      Grain Legumes Integrated Project
               Objective :
                                            WP 1.1 Potential and behaviour of
        To define the impact and
       potential of improved grain
         legumes on optimised
                                            processed products of legume seeds.
      animal feed and human food.
                                            Udo Knauf , Fraunhofer IVV, D

     Module                  Module
        1:
To identify optimal
                               2:
                         To use legumes
                                            WP 1.2 Intestinal microflora, gut health
  parameters for       to develop healthy
 legumes in feed        and sustainable     and nutritional value in animals.
    quality and            Agriculture
      safety                                Alfons Jansman, ID Lelystad BV, NL
      WP1.1                     WP2.1
Grain Legumes in             Lower Input
      Feed                     Farming
                                            WP 2.1 Agronomic considerations of grain
      WP1.2                  WP2.2
Feed Processing &         Economic &        legumes in agriculture.
 Nutritional value       environmental
                            analysis        ES Jensen, Risø, DK

                                            WP 2.2 Economic considerations of grain
                                            legumes in agriculture,including Life Cycle
                                            Analysis.
                                            T Nemecek, FAL, CH
           Grain Legumes Integrated Project
                                      Objective :
WP 3.1                                                             Impact of growth constraints
                                   To understand the
High throughput              factors affecting grain legume
                                 seed quality and use.             WP 4.1 Abiotic stress
approaches to
                                                                   Martin Crespi, CNRS Gif sur Yvette, F
characterise protein
& metabolite                Module
                               3:
                                                    Module
                                                       4:
                                                                   WP 4.2 Biotic stress
amounts &               To investigate
                       Variation in grain
                                                To identify the
                                               factors affecting   Diego Rubiales, CSIC, Córdoba, E
                        legume seed              grain legume
composition              Composition           seed composition
                                                                   WP 4.3 Plant Architecture
R Thompson,
                            WP3.1                   WP4.1          Francisco Madueño, CSIC Valencia, E
INRA Dijon, F             Systems               Environmental
                        approaches to
                       seed composition
                                               Effects on Seed
                                                    Quality
                                                                   Catherine Rameau, INRA Versailles, F
                             WP3.2                 WP4.2
                       Novel approaches       Pathogen Effects
WP 3.2                   to alter seed
                          composition
                                               on Seed Quality

Gene and allele                                     WP4.3
                                                                   WP 4.4 Control of flux from primary
discovery, including                          Plant Architecture

                                                                   assimilation to seed filling.
QTL approaches.                                     WP4.4
                                              Carbon/nitrogen      Christophe Salon, INRA Dijon, F
C Domoney,                                    allocation & seed
                                                    quality
JIC, UK
              Grain Legumes Integrated Project
M 5 Genomic tools                                                     Objective :

J Denarié, INRA Toulouse, F                                To develop genomic and post-
                                                              genomic tools needed to
                                                             improve and sustain grain
WP 5.1 Genome sequencing                                  legume seed quality and supply.


Giles Oldroyd, JIC, Norwich, UK                                                                    WP 6.1 Bioinformatics
                                                           Module                  Module

WP 5.2 Mutagenesis                                            5:
                                                       To develop new
                                                                                      6:
                                                                              To develop new
                                                                                                   Klaus Mayer, MIPS,
Adam Kondorosi,
                                                      genetic & genomic
                                                          tools for
                                                                             bioinformatic tools
                                                                             needed to improve
                                                                                                   Munich, D
                                                        grain legume            grain legume
CNRS Gif sur Yvette, F                                  improvement              seed quality

                                                           WP5.1                    WP6.1
                                                                                                   Sequence annotation,
WP 5.3 Expression profiling                              Sequencing             Bioinformatics     transcriptomic tools,
Helge Kuester, Bielefeld, D                                                                        comparative genomics,
                                                           WP5.2

WP 5.4 Crop & Comparative Genomics                      Mutagenesis                                gemplasm analysis
György Kiss, Inst. Genetics,                               WP5.3

BRC Szeged & Gödöllő, Hu
                                                         Expression
                                                          Profiling

Medicago truncatula, Lotus japonicus, pea,                 WP5.4
chickpea, faba bean, bean, lentil, lupin, Phaseolus    Crop & Compar.
                                                          Genomics
and clover
  Grain Legumes Integrated Project
                                                         Objective :
                                               To coordinate and integrate
                                                  grain legume research,
                                                   to provide training in
WP 7.1 Coordination                        emerging technological approaches,
                                                  to disseminate results,
Noel Ellis, JIC, UK                         and transfer technology to industry



                                               Module                Module
WP 7.2 Short term fellowships & training           7:
                                             To provide
                                                                        8:
                                                                 To disseminate
                                           coordination &         knowledge &
workshops                                     training in      transfer technology
                                            grain legume
Ton Bisseling, WAU, Wageningen, NL             research

                                               WP7.1                 WP8.1
                                            Coordination          Dissemination

WP 8.1 Dissemination of Knowledge.
                                               WP7.2                 WP8.2
A Schneider, AEP, Paris, F                    Training               Transfer




WP 8.2 Transfer and Exploitation of
Results
C Golstein, TTP, GLIP

M 8 F Muel, UNIP, F
                                                 Integration
 Grain Legumes Integrated Project



Dissemination                            Bioinformatics
  & transfer
                        Comparative
                     Genetics & Genomics


                   Seed                Crop
                composition         performance
                              Genetics

        Use                                       Production

                         Coordination
                          & training
Grain Legumes Integrated Project
     15             10   5            25   50       75
                             WP No.
                              1.1
% of total effort                               % complete in
                              1.2
                                                the first 18 months
                              2.1
                              2.2

                              3.1
                              3.2

                              4.1
                              4.2
                              4.3
                              4.4

                              5.1
                              5.2
                              5.3
                              5.4

                              6.1

                              7.1
                              7.2

                              8.1
                              8.2                        14.4 M€ award
                               all                            over 48 months
                                                         24.8 M€ total costs
Grain Legumes Integrated Project

        Distribution of funding by species
      chickpea     lentil   clover   Phaseolus

           lupin

     faba bean



     pea                                 Medicago truncatula




Lotus japonicus
Grain Legumes Integrated Project



1. The problem
2. The approach
3. The partnership
4. The outputs
5. Potential interactions
      Grain Legumes Integrated Project
50 Partners    ~60 labs, 8 SMEs              14.4 M€ award over 48 months
17 Countries    10 EU                        24.8 M€ total costs
                 2 EEA (+ CH)
                 3 New Member States         50% budget
                 1 Third country             10 partners




                         Participating Laboratory
                         Laboratory collaborating, or mentioned in the Technical Annexe
          Grain Legumes Integrated Project
                             Number of participants by Country

3 New Member States
                                             Po Cz S
                   2 EEA                NP               D
                                   Is
                               I
                         CH
                        Hu

                    B

1 Third country                                                  UK
                  Aus


                        NL


                              DK
                                                          E

                                                F
Grain Legumes Integrated Project



1. The problem
2. The approach
3. The partnership
4. The outputs
5. Potential interactions
 Grain Legumes Integrated Project

Realistic assessment Comprehensive description of
 of the impact and use  Grain Legume seed components
  of Grain Legumes         their variability and impact
      in animal feed              Identification of
 Definition of the use of        genetic resources
 Grain Legumes in crop counter the main constraints
                         to
rotations for sustainable in Grain Legume production
         agriculture:
             Integrated         Genome
  Life cycle assesment
                           for
      bioinformatics toolssequence to anchor
                      Grain and
   Grain Legume geneticsLegume genetic maps.
              genomics Mutant populations and
                      genomic tools for systematic
                             post genomics
Grain Legumes Integrated Project



1. The problem
2. The approach
3. The partnership
4. The outputs
5. Potential interactions
Grain Legumes Integrated Project
WP 7.2 Training
Training fellowships:
     Mobility within the IP
     External connections of the IP (including TTP)

     18 short term fellowships per year
     see www.eugrainlegumes.org for application detaills


Workshops and Courses:
     A workshop on bioinformatics (for breeders and young
     scientists) will be organised in months the 12-18

      A course on the model legume Medicago truncatula
      is scheduled in the second year (month 13 to 18).

      An Ascochyta workshop.
        Grain Legumes Integrated Project



                  IP   TTP

participants                     commercial breeders

           TTP                   end-users (feed companies)

                                 other projects
                                              National projects,
                                              CP in CGAIR?

                 anyone needing access
                  to this technology
www.eugrainlegumes.org
        12       1        2

                                              1   Lens culinaris (macrosperma)
                                              2   Lotus japonicus (Gifu)
                                              3   Vicia narbonensis
                                              4   Lupinus mutabilis
11                                        3   5   Cicer arieteneum (Kabuli)
                                              6   Phaseolus vulgaris
                                              7   Pisum sativum (rr)
                                          4   8   Lens culinaris (microsperma)
10                                            9   Medicago truncatula
                                                  (R108::Tnt1)
                                          5   10 Vicia faba
                                              11 Cicer arieteneum (Desi)
9                                             12 Pisum sativum (RR)
                                              Background:
                                              faded pea standard petal (arar)

             8       7        6

     Thanks to: Mike Ambrose, Andrew Davies, Julie Hofer, Trevor Wang

				
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