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					                Laurel Wilt Update
             Statewide Pest Polycom Update

             Dr. Jonathan H. Crane, Tropical Fruit
                         Crop Specialist
                   University of Florida, IFAS
           Tropical Research and Education Center
                     Homestead, Florida

9/8/2011
  Current spread of Laurel Wilt (LW)




9/15/2012
            Location of the LW positive trees in
                   Miami-Dade County
• Bird Road Basin                      • Samples were sent to 3
      – Natural area                     laboratories
      – Numerous land owners              – DPI, Gainesville
      – Well-field recharge location      – J. Smith, UF-SFRC
• RAB trapped Feb., 2010                  – R. Ploetz, UF-TREC
• Three swampbay (Persea               • Confirmed by:
  palustris) trees were sampled           – Visual – CSMA selective augur -
  and found positive for LW,                symptoms
  Feb., 2011.                             – Molecular testing
                                              • PCR amplification of diagnostic
• Between mile marker ~20 and                   small subunit (rDNA)
  21 on east side of Krome                    • PCR amplification of diagnostic
  Avenue. About 4 miles to the                  microsatellite DNA loci
  nearest avocado grove, 15               – Koch’s postulates
  miles to the main production                • Inoculate container-grown
                                                ‘Simmonds’ avocado trees with
  area.                                         isolates from suspect trees


9/15/2012
               Current status
• LW was positively identified in about 30
  additional swampbay samples in the Bird Road
  Basin and numerous trees show the wilting/dead
  leaf symptoms.
• The disease (insect) appears to be spreading
  mostly north and west into the natural areas.
  There has been some southern spread on native
  trees but not beyond SW 88 St. (Kendall Drive).
• LW has not been detected in any avocado trees in
  the urban areas to the east or groves to the
  south.

9/15/2012
  Location of the LW
       positive
   swampbay trees
in Miami-Dade County

               Tamiami Trail/
               Rt. 41/SW 8th St.

                  SW 88 St.
                  (Kendall Drive)




  Agricultural/avocado
  production area




9/15/2012
      Swampbay – just south of mi. marker 20 and between mi.
          marker 20-21 along SW 177 Ave. (Krome Ave.)




    = dying tree
9/15/2012
            Swampbay along SW 42 St. canal (off of SW 162 Ave.)




                                                          = dying tree
9/15/2012
              Swampbay along SW 42 St. canal (off of SW 162 Ave.)




                                                         Sapwood
                                                         discoloration

Sawdust
toothpicks                     Sawdust




  9/15/2012
Avocado symptoms
Brevard County




9/15/2012
              Current recommendations
Urban/residential                  Commercial avocado industry
• Report declining trees in        • Detection and suppression
  Lauraceae to DPI (1-888-397-        – Early detection of LW and
  1517)                                 suppression of RAB
• Remove declining or dead host       – Remove and destroy
  wood but do not move from
                                        infested/infected wood or
  property (unless to
  incinerator)                          tree
      – Chip/tarp, burn, or bury   • Limited area insecticide
• Alamo® (propiconazole) is          applications upon detection
  legal for infusion into redbay      – Danitol®, Permethrin®,
  trees                                 Malathion®, Admire®
• Merit® and Malathion® are        • No fungicide
  registered
      – When to apply?
                                     recommendations at this
      – Environmental issues         time (Tilt® is legal but…)

9/15/2012
             Current research - TREC
Plant Pathology (RC Ploetz)          Entomology (JE Peña)
• Biology of LW                      • Insecticide efficacy
      – Host-pathogen interactions      – Contact
      – Physiology of avocado           – Systemics
        reaction
                                        – Repellents
      – LW Epidemiology
• LW avocado cultivar                • Biological control
  evaluations                        • Biology of RAB
• Fungicide infusion testing            – Flight behavior and host
  under grove conditions                  selection
      – Propiconazole                   – Investigating attack severity
      – Thiabendazole                     to disease incidence
      – Tebuconazole
9/15/2012
                        Current research
Plant pathology                         Entomology
• J Smith, UF-SFRC                      • L Stelinski et al., UF-CREC
      – Diagnostics                        – Host RAB attractants (host
                                             plant volatiles, fungal
      – Transmission of LW                   volatiles, others) and host
      – Effect of chipping/composting        attractiveness
        on RAB and LW survival          • P Kendra, USDA-ARS
                                           –   Host RAB attractiveness
   Agricultural economics
                                           –   RAB boring behavior/cues
   • E Evans, UF-TREC
                                           –   Host wood volatile analysis
            - Economic impact
            - Cost of control              –   RAB flight behavior
                                           –   Trap and kill systems


9/15/2012
                  J Crane - Extension-research
Extension                            Research
• In cooperation with FDACS-DPI      • Entomology
  and UF-Extension Agents
                                        – Assist chemical control
• Laurel Wilt Working Group
                                          investigations with Dr. Peña
• Avocado Administrative
  Committee and Industry             • Plant pathology
      – Liaison, reports, updates,      – Fungicide infusion evaluation
        advice
                                          team with Rainbow Tree Care
      – Control strategy
                                          (Tom Prosser/Greg Krogstad)
• In-person seminars,                     and Dr. Ploetz and Dr. Evans
  webinars/polycom, brochures,
  postcards, print and electronic           • Fruit residues
  documents and presentations               • Efficacy
• Research funding advocate
  and liaison

9/15/2012
                        FDACS/DPI Helpline
                          888-397-1517
                               DPI links:
                             www.fl-dpi.com
http://www.freshfromflorida.com/pi/enpp/pathology/laurel_wilt_disease.html
                      savetheguac.com

                         UF/IFAS Extension offices:
            http://solutionsforyourlife.ufl.edu/map/index.html

               UF/IFAS publications: http://edis.ifas.ufl.edu

            UF/IFAS Tropical Research and Education Center:
                        http://trec.ifas.ufl.edu

9/15/2012

				
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