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					Identification of microRNA function by target
     prediction and expression profiling




                 Youngae Lee
         MicroRNA (miRNA)

• A new family of small noncoding RNA (~22 nt)
• ~250 miRNA in animal
• Inhibition of translational (animal) or Degradation of
target RNA (plants)
• Regulation of cell proliferation, differentiation,
apoptosis, carcinogenesis and viral infection
• Unknown function of newly identified animal miRNA
The model for biogenesis and post-transcriptional
      suppression of miRNA and siRNA
             Purpose

• Computational target gene prediction

• Identification of microRNA function
• Expression profiling
                  Methods

• 3’-UTR sequence retrieval; ENSEMBL website
 http:// www. ensembl.org
• The mature miRNA sequence
 http://www.sanger.ac.uk/Software/Rfam/mirna/
• Identify the targets of miRNAs
 http://genes.mit.edu/targetscan
• Gene Ontology (GO) annotations
 http:// geneontology.org/
                Results


1. miRNA target prediction algorithm
2. mRNA profiling and function
                        References

1. Azra Krek et al., Nature genetics, 2005, Vol 37, 495
2. Marianthi Kiriakidou et al., Genes and Development, 2006, Vol 18,
    1165
3. T. Hubbard et al., Nucleic Acids Research, 2005, Vol 33, D447
4. Victor Ambros. Nature, 2004, Vol 431, 350
5. Xiaowei Wang et al., Nucleic Acids Research, 2006, Vol 34, 1646
6. Benjamin P. Lewis et al., Cell, Vol 115, 787

				
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posted:9/14/2012
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