Post-Processing of Corning Ultra GAPS Oligo Arrays AND SCHOTT aminosilane oligo arrays Revised Martin Buess 1-17-06 according to Corning ultra gaps and SCHOTT original protocol 1. Using a diamond scribe, etch the non-printed side of the array to denote the boundaries of the printed area. 2. UV cross-link printed DNA onto glass substrate with 65mJ of energy in a Stratalinker (650 x 100uJ) and transfer arrays on a slide rack. 3. Prehybridization in 5x SSC, 0.1 mg/ml BSA, 0.1% SDS at 42 C for 60 Min. (300 ml MQ H2O, 100 ml filtered 20 x SSC, 4 ml 10mg/ml BSA stock solution (filtered), 4 ml 10% SDS, prewarm in 42C waterbath for 60 min) 4. Rinse in arrays in 0.1 x SSC for 5 min at RT 5. Repeat step 4 6. Transfer arrays to MQ water and incubate at RT for 30 sec. 7. Spin dry arrays in a table to centrifuge at 500 rpm for 5 min and use arrays the same day.
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