The Use of Self-Patterned Phospholipid Films for Directed Enzyme Lithography
N.Y.-W. TANG and A. Badia, Department of Chemistry, University of Montreal,
C.P. 6128, succursale Centre-ville Montreal, Qc, H3T 1J4, email@example.com
Objectives Langmuir-Blodgett Monolayers of DPPC/DLPC Phospholipases
Generate phospholipid-based surface patterns from phase-separated monolayers
using the Langmuir-Blodgett technique
PLA2, a calcium-dependent and interfacially-active
Study the effect of lineactants on the pattern morphology
enzyme, catalyzes the cleavage of the sn-2 acyl ester
linkage of glycerophospholipids, yielding a fatty acid and
Demonstrate that a striped phospholipid bilayer prepared by the
Langmuir-Blodgett technique can be used for enzyme nanolithography
5 m 5 m 7.5 m
The naturally occuring L form of the lipid is hydrolyzed,
PLA2 binds but does not cleave the D form.
Structures of Phospholipids and Lineactant
DPPC, C16; Tm = 41°C 20 μm 20 μm 20 μm
PLA2 Degradation of a Striped D--DPPC/L--DLPC Bilayer
DLPC, C12; Tm = -1 °C Stripe Formation Mechanism
Langmuir Monolayers of Phospholipids
barrier barrier 100% DLPC 5
50 0.25 DPPC
100% DPPC nm nm
20 -5 -4.5
0 10 m 0 10 m
0 P. Moraille and A. Badia, Langmuir 2002, 18, 4414-4419
liquid condensed 30 40 50 60 70 80 90 100 110
Area (Å /molecule)
PLA2 Degradation of a Striped L--DPPC/ DEPC Bilayer
Solid-Supported Phospholipid Films
Effect of Cholesterol Lineactant on the Domain
Morphology for a 50/50 DPPC/DLPC Mixture
mica at = 32 mN/m
bilayer 2% chol. 5% chol. 10% chol.
deposition monolayer 5 m 5 m
Mica substrate mica
2% chol. 5% chol. 10% chol.
Atomic Force Microscopy (AFM) 5 m 5 m
Characterization of the Surface Morphology
Conclusions and Future Work on Enzyme Nanolithography
PLA2 stereospecifically hydrolyzes the L--phospholipid enantiomer in bilayer templates.
Tapping Mode PLA2 only hydrolyzes dialkylphosphatidylcholines in bilayer templates.
Enzymes make interesting nanolithographic tools due to their ability to catalyze
chemical reactions with high efficiency and specificity under mild conditions and The regularity of the monolayer and bilayer stripe patterns will be improved.
The PLA2 enzyme performance will be demonstrated for different enantiomer templates .
The stereospecificity exhibited by certain enzymes can be exploited to spatially direct
their activity to certain regions of a patterned surface, as shown herein. Alkyl tail-functionalized phospholipids will be used to spatially direct the deposition of metal nanoparticles
for the generation of 2D optical gratings.
We have investigated the phospholipase A2 (PLA2 ) hydrolysis of stereochemically-differentiated
DPPC/DLPC and chemically-differentiated DPPC/DEPC bilayers.