Experimental Design Worksheet by 6Xp9SW6

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  Yeast Respiration of Sugars Lab: Experimental Design Worksheet

Background:

Yeast are living things that are able to metabolize some foods, but not others. In
order for an organism to make use of a potential source of food, it must be
capable of transporting the food into its cells. It must also have the proper
enzymes capable of breaking the food’ s chemical bonds in a useful way.
Sugars are vital to all living organisms. Yeast are capable of using some, but not
all sugars as a food source.

Remember, that in order to survive, yeast must release the chemical energy of
glucose through a chemical reaction. This reaction is called cell respiration and
occurs in all living things. The equation for cell respiration (an exothermic
chemical reaction) is as follows:

              Glucose + oxygen - carbon dioxide + water + energy

In this lab, you will try to determine whether yeast are capable of metabolizing a
variety of sugars. When yeast carry out cell respiration, oxygen gas is consumed
and carbon dioxide, CO2, is produced. You will use a CO2 Gas Sensor to monitor
the production of carbon dioxide as yeast respire using different sugars.

The four sugars that will be tested are glucose (blood sugar), sucrose (table
sugar), fructose (fruit sugar), and lactose (milk sugar). Sugars and starches are
types of carbohydrates that are a source of immediate energy to living things.
Glucose and fructose are examples of monosaccharides, while lactose and
sucrose are types of disaccharides. Starch, a polysaccharide, is not being tested
in this lab experiment.
                    Experimental Design Worksheet

Title:

Independent variable (IV):

Levels of Independent variable:

Dependent variable (DV):

Number of trials:

Constants:

Design diagram (below):

IV:_____________________________




DV:___________________________

Constants: ____________________
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