Mutations Worksheet Activities One and Two Activity One: What is a Mutation? 1. Go to learn.genetics.utah.edu/archive/mutations/index.html 2. Read this page. 3. Click on the “Mutate a DNA Sequence Activity” (yellow box; upper right) 4. Read the yellow boxes to the right and define the following terms: Point mutation: Missense Mutation: Nonsense Mutation: Silent Mutation: Frameshift mutation (insertion/deletion): 5. Transcribe and translate the gene below. Use the genetic code I gave you. DO NOT USE THE GENETIC CODE ON THE WEBSITE! Use the definitions above to help you with the questions below. DNA SEQUENCE GCATACGCTGCGAAAGTTTGGCTGACTGGGCAATTG RNA TRANSCIPT ______ ______ ______ ______ ______ ______ ______ ______ a. What is the amino acid sequence encoded by the DNA sequence? (assume this is in the middle of a gene) _______ - _______ - _______ - _______ - _______ - _______ - _______ b. What does a missense mutation look like? How does it change the amino acid sequence? c. What does a nonsense mutation look like? How does it change the amino acid sequence? d. How do frameshift mutations change the amino acid sequence? e. Notice how a single amino acid can be encoded by a number of different codons. What are some examples of silent mutations in the DNA sequence? Activity Two: The Mighty Mutation Maker 1. Go to www.nature.ca/genome/04/0413_e.cfm#010 2. Read the introductory paragraph (starts with “Turn your name into a DNA sequence”). 3. Enter your name and click the “DECODE” button. 4. Write down your DNA “codon” sequence. What is your name code for? ___________________________________________________ 5. For each mutation at the left, see how it affects your “protein”. Report the codon changes. Make sure you click the “DECODE” button in between each mutation to reset your name. Missense: ____________________________________________________________________ Nonsense: ____________________________________________________________________ Silent: _______________________________________________________________________ Insertion: _____________________________________________________________________ Deletion: _____________________________________________________________________ 6. Summarize the explanation given in the “What Happened?” box in one-two sentences for each. Missense: ____________________________________________________________________ _____________________________________________________________________________ Nonsense: ____________________________________________________________________ _____________________________________________________________________________ Silent: _______________________________________________________________________ _____________________________________________________________________________ Insertion: _____________________________________________________________________ _____________________________________________________________________________ Deletion: _____________________________________________________________________ _____________________________________________________________________________ Homework Complete any activity you were unable to finish in class today. Complete a Clock Question 10 about the topic of mutation.
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