Qualitative Data Analysis

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Qualitative Data Analysis Powered By Docstoc
					Sequence Automation
In This Section, We Will Discuss:

 How to set up a sequence.

 How to perform automated recalibrations.

 How to do bracketing.

 How data files are named.

 How to use Sequence Summary.

 How to run the sequence.

 The sequence container (folder).



                                             2
Building a Sequence for Automated Analysis
              1. Go to the Method and Run Control view.

              2. Go to View > Preferences and set up sequence folder
                 naming convention.

              3. From the Sequence menu, select New Sequence.

              3. Edit the Sequence Parameters.

              4. Edit the Sequence Table.

              5. Select a Sequence Output.

              6. Save the Sequence

              7. Run the Sequence form the RunControl menu or
                 the Sequence Table.

             Note: Hypersequence is a feature for the Agilent 1100/1200
             Series Well plate Handler only.
                                                                          3
Preferences


              •Specifies naming convention for sequence
               data containers.




                                                      4
Sequence Parameters




                      5
Explicit Calibration


     Calib             Calib             Calib
   Standards Samples Standards Samples
                                       Standards




         Update                     Update                     Update
        Calibration                Calibration                Calibration
                      Calculate                  Calculate
                        Sample                     Sample
                      Amounts                    Amounts
                      After Each                 After Each
                       Injection                  Injection
                                                                            6
Sequence Table Explicit Recalibration


                                        Additional
                                        Columns in
                                        Sequence Tables:

                                        •Sample Amount
                                        •ISTD Amount
                                        •Multiplier
                                        •Dilution
                                        •Data file
                                        •Inj Volume
                                        •Lims ID
                                        •AutoBalance




                                                     7
Customize Sequence Table




                           8
Explicit Plus Cyclic Recalibration




                                     9
Bracketing Calibration
      Injection


       Calib             Calib             Calib
     Standards Samples Standards Samples
                                         Standards




                   Update         Update
                  Calibration    Calibration


                  Calculate      Calculate
                   Sample         Sample
                  Amounts        Amounts
                  For Each       For Each
                  Injection      Injection




                                                     10
Bracketed Sequence Operation




                               11
Partial Sequence Preview of Bracketing




         Will show you the order of injections.




                                                  12
Fill in Sequence Table Using Sequence Table
Fill Down Wizard




                                              13
Sequence Output




                  A Sequence Summary Report
                  allows you to select from a
                  simple one page Sample
                  Summary to a comprehensive
                  report documenting all aspects
                  of the analysis including cross-
                  sample statistics.
                                                     14
Edit Sequence Summary




                        Statistics calculated in
                        Standard or Extended
                        format.




                                                   15
Extended Statistics




                      16
Save the Sequence File




                         Save Current Sequence



                                                 17
Sequence Sampling Diagram




                   Shows sequence tray set-up and
                   sequence processing status. Click to
                   set-up sequence. Select Start to
                   begin sequence.

                   Select from the View menu.



                                                    18
 Start a Sequence

                                  •RunControl menu
                                  •Start button
                                  •F6
                                  •Sequence Table
                                  •Partial Sequence Table

•When you start a sequence, a sequence folder is created in the DATA
 directory.

•The sequence folder is named according to the subdirectory specified in
 Sequence Parameters, and the naming convention specified in Preferences.

•A copy of the master methods (Chem32\1\Methods\names.M) and sequence
 template file (Chem32\1\Sequences\name.S) are copied to the folder.
 The sequence acquisition is run from these copies.

                                                                       19
Sequence Folder Data Structure
                       Sequence Container (Folder)
           Subdirectory sequencetemplatename year-month-day hour-minute-second


      C:\Chem32\1\DATA\03-15-06\CLASSSEQUENCE 2006-03-15 10-46-09




               Acquired Data




                                                            Copy of Master Method
  Batch file for Batch Review

                                                             Sequence Logbook
 Copy of Sequence Template

                                                                                 20
Sequence Data Files
   Format for Automatic Injection: 003-0101
        003 Vial Number
        01 Sequence Line
        01 Replicate

   Prefix
            Prefix000001
            Prefix000002, etc. for a total of 15 characters

   Format for a Cyclic Calibration Standard: C1-02001
        C Indicates a cyclic calibration standard was used
        1 Calib Line number
        02 Sequence Line
        001 Replicate

                                                              21
Recalibration Settings




                         22
Calibration Report Options




                             23

				
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posted:9/2/2012
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