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Budget-Nature Works V1A

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Budget-Nature Works V1A Powered By Docstoc
					Original FedDev Plan

Task                                      Due date    Fed Dev Cash    SME in-kind
1) Take 5 matrix samples at different      15-Dec-10           $2,500                      $2,500
depths from ABR 1 for digestion and assay
for total arsenic
2) Examine samples under TEM                31-Jan-11          $2,500                      $1,000
3) Operate ABR 1 & 2 (full system) for       31-Mar-11             $7,500                  $2,500
1 month
4) Sample 3 ports (bottom, middle, top)      31-Mar-11             $7,500                  $3,000
once a week for a month
Subtotal for year ending Mar 31, 2011                    Total $20,000      Total $9,000 labour
                                                         $15,000 labour
                                                         $5,000 supplies
5) Determine the organic and inorganic       30-Sep-11            $15,000                  $8,000
arsenic species in a controlled bioreactor
system. Derive a mass balance, including
loss of As from the system due to
volatilization.
                                                       (10,000 labour) Labour
                                                       5000 supplies
6) Isolate microorganisms from the           31-Mar-12           $15,000 8000 Labour
system. Determine optimal growth
conditions. DNA extraction then DGGE
(U of G)       Sub Contracted
Subtotal for year ending Mar 31, 2012                             $30,000                 $16,000
Totals                                                            $50,000                 $25,000
    Stephanie's estimates
    Arsenic Project
                                                          Total $                  Labour              Material             Time in Hours
    Grand Total Estimate of Weekly Costs                              $1,177.50              $719.70              $457.80              22.95
    Water Quality Analysis:                                           $1,011.00              $553.20              $457.80            1107.00 minutes
    System checking and Sampling                                        $166.50              $166.50                                  270.00 minutes
    External Lab Analysis (Derk)                                    see next tab
    Details:
    Output samples:
    Number of Samples per week :                5

    Parameter                              Test Time            Supplies             Data Reporting    Labour $             Supply $        Total$         Total Time
                                         Min per sample     cost per sample          Min per sample                                                        min
A   metals - As, Fe, Zn, S                     10                $20.00                    5                $10.00              $20.00           $30.00            15
B   pH                                          2                 $0.10                    2                 $1.47               $0.10            $1.57             4
B   DO                                          2                 $0.10                    2                 $1.47               $0.10            $1.57             4
    Total Requirements:                        70               $101.00                    45               $64.67              $101.00          $165.67          115

    Anoxic Side Port samples: samples to be purged with nitrogen gas first.

    Number of Samples per week :               16

    Parameter                              Test Time            Supplies             Data Reporting    Labour $             Supply $        Total$         Total Time
                                         Min per sample     cost per sample          Min per sample                                                        min
B   ORP                                        5                 $0.10                     2                $2.57                $0.10            $2.67            7
B   pH                                         5                 $0.10                     2                $2.57                $0.10            $2.67            7
B   DO                                         5                 $0.10                     2                $2.57                $0.10            $2.67            7
    Total Requirements:                       240                $4.80                     96              $123.20               $4.80           $128.00          336
    Anoxic Side Port samples: samples to be purged with nitrogen gas first.

    Number of Samples per week :               16

    Parameter                              Test Time            Supplies      Data Reporting   Labour $      Supply $      Total$        Total Time
                                         Min per sample     cost per sample   Min per sample                                             min
B   Sulfide ion                                 6                $0.50              2                $2.93        $0.50          $3.43           8
    Total Requirements:                        96                $8.00              32              $46.93        $8.00         $54.93          128

    Anoxic Side Port samples: samples to be purged with nitrogen gas first.

    Number of Samples per week :               16

    Parameter                              Test Time            Supplies      Data Reporting   Labour $      Supply $      Total$        Total Time
                                         Min per sample     cost per sample   Min per sample                                             min
A   Fe total (HACH)                             5                 $0.50             2               $4.67         $0.50         $5.17             7
A   Fe 2+ (HACH)                                5                 $0.50             2               $4.67         $0.50         $5.17             7
A   metals ICP-OES - As, Zn, Fe, S             10                $20.00             2               $8.00        $20.00        $28.00            12
    Total Requirements:                        80               $336.00             96             $277.33       $336.00       $613.33          416

    Anoxic Side Port samples: samples to be purged with nitrogen gas first.

    Number of Samples per week :               16

    Parameter                              Test Time            Supplies      Data Reporting   Labour $      Supply $      Total$        Total Time
                                         Min per sample     cost per sample   Min per sample                                             min
B   Prep for Dirk- addition of EDTA?            5                $0.50              2                $2.57        $0.50          $3.07           7
    Total Requirements:                        80                $8.00              32              $41.07        $8.00         $49.07          112
    Item                            Part Number        Supplier          Cost   Units Required   Total with Taxes
                                                                                       1               $0.00      Per 25vials
                                                                                       1               $0.00
                                                                                       1               $0.00
                                                                                       1               $0.00
                                                                                       1               $0.00
                                                                                       1               $0.00      each
                                                                                       2               $0.00
                                                                                       1               $0.00

    Total                                                                                                   $0.00

A   Technician                                    40
B   Technologist                                  22
C   Student                                       11              Total Check          $553.20           $457.80          $1,011.00   1,107.00

    Other Time per week
A   Zinc barrel check and filling              60                                   $40.00
A   Enter cage check system                  120                                    $80.00
A   Fill system                                45                                   $30.00
B   Fill system                                45                                   $16.50
    Total                                 270.00                                   $166.50                                             270.00
cost estimate Fleming As speciation project                                                                        Total    Updated April 14 detail
                                                       Updated 01/04/2011                                          Check       labour        Non-Labour
a) pore water analyses                                                               $10,800                      10,800.00     6,134.00           4,666.00

1) stability / preservation test
sampling by Trent personnel / training of Fleming personnel                   $400                                   400.00        211.00           189.00
stability test (2 analyses: same day and one week)                          $2,600                                 2,600.00      1,481.00         1,119.00

2) monitoring during experiment
6 sets of analyses (e.g. monthly or spaced unevenly)                        $7,800                                 7,800.00      4,442.00         3,358.00

b) sequential extraction                                                             $11,972                      11,927.00     10,565.00         1,362.00

1) sample preparation
20 hrs X21.25 +4% for four months (16 weeks) would be $7072.                $7,072   <===      Fleming Rates       7,027.00      7,027.00
                                                                                                                        -
2) method testing analyses for As species (incl. methylated species)
6 SeqEx steps @ $ 650 each                                                  $3,900    <==      Revised April 13    3,900.00      2,538.00         1,362.00
                                                                                                                        -
3) analysis of extracts from reactor samples                                                                            -
TAs and As speciation                                                       $1,000                                 1,000.00      1,000.00
                                                                                                                        -
                                                                                                                        -
                                                                                     $22,772                            -
Revised April 1, 2011
Dirk Wallschlaeger Request
Dirk Wallschlaeger <dwallsch@trentu.ca>
a) pore water analyses                      $10,800

1) stability / preservation test

sampling by Trent personnel /       $400
training of Fleming personnel
stability test (2 analyses:        $2,600
same day and one week)
2) monitoring during
experiment
6 sets of analyses (e.g.           $7,800
monthly or spaced unevenly)
b) sequential extraction                    $11,972
1) sample preparation
20 hrs X21.25 +4% for four         $7,072
months (16 weeks) would be
$7072.
2) method testing analyses
for As species (incl.
methylated species)
6 SeqEx steps @ $ 650 each         $3,900             revised April 13



3) analysis of extracts from
reactor samples
TAs and As speciation               1000




Total                                       $22,772
    DNA Extraction Budget – As methylation (Glasauer)
                                                4-Apr-11
                         Item                              Cost    Supplier                                          Description
Disposable tubes                                            $150 Sigma         Falcon tubes, PCR tubes
                                                                 Aldrich

Antibiotics and medium supplements                          $100 Sigma         Plasmid transformed into E. coli will contain antibiotic resistance. Plating out
                                                                 Aldrich       transformed cells onto antibiotic inoculated medium will only grow cells that have
                                                                               been successfully modified.

E. coli from ATCC                                            $50               Chemically competent E. coli strain
DNA Extraction kits                                         $400 Mo-Bio        Mo-Bio soil DNA extraction kit 50 uses (2 kits)
Genetic facilities use                                     $1,300 University   This encompasses all PCR, gel electrophoresis, and imaging facilities use. This also
                                                                  of Guelph    includes reagents such as Taq polymerase, and nucleotide stock solutions (as master
                                                                               mix), and fluorescent indicator (SyberSafe).

Restriction enzymes                                         $200 Invitrogen    Eco R1 and Hind III restriction enzymes to cut DNA at key points


DNA ligase enzyme                                           $200 Invitrogen    Ligase enzyme, in order to fuse restriction digested DNA fragments to restriction
                                                                               digested plasmid DNA.

PCR primers                                                 $200 Lab Services Primers for arsenic methylation gene conserved regions (from BLAST database). This
                                                                              will likely take some refinement, but will include restriction sites to flank the gene of
                                                                              interest. These restriction sites are specific for restriction enzymes, which cleave the
                                                                              dsDNA in a staggered manner, and allow it to be reattached using a ligase enzyme.


DNA sequencing                                              $150 Lab services DNA sequencing of PCR products after electrophoretic separation


Nanodrop facility                                           $150 Lab services Low quantity DNA quantification technique


Methylation reaction reagents                               $250 Sigma         Necessary substrates for methylation reaction (after plasmid is cloned into E. coli and
                                                                 Aldrich       protein is expressed and functional).
TLC separation reagents                                $100 Sigma        To separate methylated arsenic species using the method of Styblo et al (1995) J.
                                                            Aldrich      Chrom. B 668: 21-29, using thin layer chromatography for quick confirmation

Plasmid kit                                            $700 Invitrogen   Necessary for transfer of target gene from DNA extractions and PCR amplification to
                                                                         E. coli

DNA ladder for gels                                    $200 Invitrogen   DNA ladder to identify molecular weight of DNA products on an electrophoresis gel


Travel to Fleming                                      $330              Car rental and per diem for 3 trips
Total                                                $4,380

Update by Susan April 7, Reduce the total by 1,500   -$1,500             molecular kits, share them between projects, That would allow us to trim 1500

Total                                                $2,880


                                                       $750
           microbiology work             $ 10,836
           reagents            $ 2,880
           Labour Uof G        $ 7,956              Sean




           Total UofG                    $ 10,836

Revised April 7, 2011
         Summary

         Original FedDev Plan                                 New Requirements


Fleming Labour      $ 25,000 50%              20% Fleming Labour              10,076    7 months remaining, sampling
Fleming Material    $ 10,000 20%              13% Fleming Material             6,409    2 times per month
Guelph UofG         $ 15,000 30%              22% Guelph UofG                 10,836

                                              45% Trent       Dirk            22,772

Totals              $ 50,000                                              $ 50,093


                                                                          $      93       over budget


         Fed Dev ARCI funding is $50,000 of cash with $25,000 of in-kind services donated by Nature Works for this funding. Activities must be completed by March 31, 2012.
         The deadline to start was originally March 31, 2011 but has been extended to June. But we want to get started asap.
         Nature Works and Kinross are also partners under my NSERC IE grant and are eligible for support under it.

         Problem is that Fed Dev has been extremely clear that we cannot use funding from other projects to extend or enhance what their funding is doing in any way.
         If we do use funding to top up the Fed Dev project and they catch us they'll remove that amount from the Fed Dev award.
         We have to have a discrete $50,000 project. After it is finished we can do what we like for a different project under a different name.

         Other issues - I cannot send any funding from my NSERC grant to universities. So I would have to employ any labour directly from Fleming.
         Fleming is unionized and our pay rates are higher so this is a disincentive if the budget is tight.

         What we need to do is find a 3-6 month discrete project that costs $50,000 (with in-kind services provided by Nature Works so we can claim the funding).
ed by March 31, 2012.




in any way.
Nature Works Remediation Project FedDev 846236

Labour
Flemng Lab Staff                             10,076.00
Uof G Labour                                  7,956.00

Trent- sampling by Trent personnel /              211.00
training of Fleming personnel
Trent - Sample prep                              7,072.00

Trent - stability test (2 analyses: same         1,481.00
day and one week)
Trent - 6 sets of analyses (e.g.                 4,442.00
monthly or spaced unevenly)
Trent - 6 SeqEx steps @ $ 650 each               2,538.00
Trent- analysis of extracts from                 1,000.00
reactor samples, TAs and As
speciation

Total Labour                                                34,776.00

Materials
Fleming Lab Materials                            6,409.00
UofG Lab Materials                                 750.00
Trent - stability test (2 analyses: same         1,119.00
day and one week)
Trent - 6 sets of analyses (e.g.                 3,358.00
monthly or spaced unevenly)
Trent - 6 SeqEx steps @ $ 650 each               1,362.00
Trent- sampling by Trent personnel /              189.00
training of Fleming personnel

Total Materials                                             13,187.00

Sub Contracts
Car Rental UofG                                    330.00
Lab Services UofG                                  500.00
Imaging Facilities UofG                          1,300.00

Total Sub Contracts                                          2,130.00

Total                                                       50,093.00

				
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