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Original FedDev Plan
Task Due date Fed Dev Cash SME in-kind
1) Take 5 matrix samples at different 15-Dec-10 $2,500 $2,500
depths from ABR 1 for digestion and assay
for total arsenic
2) Examine samples under TEM 31-Jan-11 $2,500 $1,000
3) Operate ABR 1 & 2 (full system) for 31-Mar-11 $7,500 $2,500
1 month
4) Sample 3 ports (bottom, middle, top) 31-Mar-11 $7,500 $3,000
once a week for a month
Subtotal for year ending Mar 31, 2011 Total $20,000 Total $9,000 labour
$15,000 labour
$5,000 supplies
5) Determine the organic and inorganic 30-Sep-11 $15,000 $8,000
arsenic species in a controlled bioreactor
system. Derive a mass balance, including
loss of As from the system due to
volatilization.
(10,000 labour) Labour
5000 supplies
6) Isolate microorganisms from the 31-Mar-12 $15,000 8000 Labour
system. Determine optimal growth
conditions. DNA extraction then DGGE
(U of G) Sub Contracted
Subtotal for year ending Mar 31, 2012 $30,000 $16,000
Totals $50,000 $25,000
Stephanie's estimates
Arsenic Project
Total $ Labour Material Time in Hours
Grand Total Estimate of Weekly Costs $1,177.50 $719.70 $457.80 22.95
Water Quality Analysis: $1,011.00 $553.20 $457.80 1107.00 minutes
System checking and Sampling $166.50 $166.50 270.00 minutes
External Lab Analysis (Derk) see next tab
Details:
Output samples:
Number of Samples per week : 5
Parameter Test Time Supplies Data Reporting Labour $ Supply $ Total$ Total Time
Min per sample cost per sample Min per sample min
A metals - As, Fe, Zn, S 10 $20.00 5 $10.00 $20.00 $30.00 15
B pH 2 $0.10 2 $1.47 $0.10 $1.57 4
B DO 2 $0.10 2 $1.47 $0.10 $1.57 4
Total Requirements: 70 $101.00 45 $64.67 $101.00 $165.67 115
Anoxic Side Port samples: samples to be purged with nitrogen gas first.
Number of Samples per week : 16
Parameter Test Time Supplies Data Reporting Labour $ Supply $ Total$ Total Time
Min per sample cost per sample Min per sample min
B ORP 5 $0.10 2 $2.57 $0.10 $2.67 7
B pH 5 $0.10 2 $2.57 $0.10 $2.67 7
B DO 5 $0.10 2 $2.57 $0.10 $2.67 7
Total Requirements: 240 $4.80 96 $123.20 $4.80 $128.00 336
Anoxic Side Port samples: samples to be purged with nitrogen gas first.
Number of Samples per week : 16
Parameter Test Time Supplies Data Reporting Labour $ Supply $ Total$ Total Time
Min per sample cost per sample Min per sample min
B Sulfide ion 6 $0.50 2 $2.93 $0.50 $3.43 8
Total Requirements: 96 $8.00 32 $46.93 $8.00 $54.93 128
Anoxic Side Port samples: samples to be purged with nitrogen gas first.
Number of Samples per week : 16
Parameter Test Time Supplies Data Reporting Labour $ Supply $ Total$ Total Time
Min per sample cost per sample Min per sample min
A Fe total (HACH) 5 $0.50 2 $4.67 $0.50 $5.17 7
A Fe 2+ (HACH) 5 $0.50 2 $4.67 $0.50 $5.17 7
A metals ICP-OES - As, Zn, Fe, S 10 $20.00 2 $8.00 $20.00 $28.00 12
Total Requirements: 80 $336.00 96 $277.33 $336.00 $613.33 416
Anoxic Side Port samples: samples to be purged with nitrogen gas first.
Number of Samples per week : 16
Parameter Test Time Supplies Data Reporting Labour $ Supply $ Total$ Total Time
Min per sample cost per sample Min per sample min
B Prep for Dirk- addition of EDTA? 5 $0.50 2 $2.57 $0.50 $3.07 7
Total Requirements: 80 $8.00 32 $41.07 $8.00 $49.07 112
Item Part Number Supplier Cost Units Required Total with Taxes
1 $0.00 Per 25vials
1 $0.00
1 $0.00
1 $0.00
1 $0.00
1 $0.00 each
2 $0.00
1 $0.00
Total $0.00
A Technician 40
B Technologist 22
C Student 11 Total Check $553.20 $457.80 $1,011.00 1,107.00
Other Time per week
A Zinc barrel check and filling 60 $40.00
A Enter cage check system 120 $80.00
A Fill system 45 $30.00
B Fill system 45 $16.50
Total 270.00 $166.50 270.00
cost estimate Fleming As speciation project Total Updated April 14 detail
Updated 01/04/2011 Check labour Non-Labour
a) pore water analyses $10,800 10,800.00 6,134.00 4,666.00
1) stability / preservation test
sampling by Trent personnel / training of Fleming personnel $400 400.00 211.00 189.00
stability test (2 analyses: same day and one week) $2,600 2,600.00 1,481.00 1,119.00
2) monitoring during experiment
6 sets of analyses (e.g. monthly or spaced unevenly) $7,800 7,800.00 4,442.00 3,358.00
b) sequential extraction $11,972 11,927.00 10,565.00 1,362.00
1) sample preparation
20 hrs X21.25 +4% for four months (16 weeks) would be $7072. $7,072 <=== Fleming Rates 7,027.00 7,027.00
-
2) method testing analyses for As species (incl. methylated species)
6 SeqEx steps @ $ 650 each $3,900 <== Revised April 13 3,900.00 2,538.00 1,362.00
-
3) analysis of extracts from reactor samples -
TAs and As speciation $1,000 1,000.00 1,000.00
-
-
$22,772 -
Revised April 1, 2011
Dirk Wallschlaeger Request
Dirk Wallschlaeger <dwallsch@trentu.ca>
a) pore water analyses $10,800
1) stability / preservation test
sampling by Trent personnel / $400
training of Fleming personnel
stability test (2 analyses: $2,600
same day and one week)
2) monitoring during
experiment
6 sets of analyses (e.g. $7,800
monthly or spaced unevenly)
b) sequential extraction $11,972
1) sample preparation
20 hrs X21.25 +4% for four $7,072
months (16 weeks) would be
$7072.
2) method testing analyses
for As species (incl.
methylated species)
6 SeqEx steps @ $ 650 each $3,900 revised April 13
3) analysis of extracts from
reactor samples
TAs and As speciation 1000
Total $22,772
DNA Extraction Budget – As methylation (Glasauer)
4-Apr-11
Item Cost Supplier Description
Disposable tubes $150 Sigma Falcon tubes, PCR tubes
Aldrich
Antibiotics and medium supplements $100 Sigma Plasmid transformed into E. coli will contain antibiotic resistance. Plating out
Aldrich transformed cells onto antibiotic inoculated medium will only grow cells that have
been successfully modified.
E. coli from ATCC $50 Chemically competent E. coli strain
DNA Extraction kits $400 Mo-Bio Mo-Bio soil DNA extraction kit 50 uses (2 kits)
Genetic facilities use $1,300 University This encompasses all PCR, gel electrophoresis, and imaging facilities use. This also
of Guelph includes reagents such as Taq polymerase, and nucleotide stock solutions (as master
mix), and fluorescent indicator (SyberSafe).
Restriction enzymes $200 Invitrogen Eco R1 and Hind III restriction enzymes to cut DNA at key points
DNA ligase enzyme $200 Invitrogen Ligase enzyme, in order to fuse restriction digested DNA fragments to restriction
digested plasmid DNA.
PCR primers $200 Lab Services Primers for arsenic methylation gene conserved regions (from BLAST database). This
will likely take some refinement, but will include restriction sites to flank the gene of
interest. These restriction sites are specific for restriction enzymes, which cleave the
dsDNA in a staggered manner, and allow it to be reattached using a ligase enzyme.
DNA sequencing $150 Lab services DNA sequencing of PCR products after electrophoretic separation
Nanodrop facility $150 Lab services Low quantity DNA quantification technique
Methylation reaction reagents $250 Sigma Necessary substrates for methylation reaction (after plasmid is cloned into E. coli and
Aldrich protein is expressed and functional).
TLC separation reagents $100 Sigma To separate methylated arsenic species using the method of Styblo et al (1995) J.
Aldrich Chrom. B 668: 21-29, using thin layer chromatography for quick confirmation
Plasmid kit $700 Invitrogen Necessary for transfer of target gene from DNA extractions and PCR amplification to
E. coli
DNA ladder for gels $200 Invitrogen DNA ladder to identify molecular weight of DNA products on an electrophoresis gel
Travel to Fleming $330 Car rental and per diem for 3 trips
Total $4,380
Update by Susan April 7, Reduce the total by 1,500 -$1,500 molecular kits, share them between projects, That would allow us to trim 1500
Total $2,880
$750
microbiology work $ 10,836
reagents $ 2,880
Labour Uof G $ 7,956 Sean
Total UofG $ 10,836
Revised April 7, 2011
Summary
Original FedDev Plan New Requirements
Fleming Labour $ 25,000 50% 20% Fleming Labour 10,076 7 months remaining, sampling
Fleming Material $ 10,000 20% 13% Fleming Material 6,409 2 times per month
Guelph UofG $ 15,000 30% 22% Guelph UofG 10,836
45% Trent Dirk 22,772
Totals $ 50,000 $ 50,093
$ 93 over budget
Fed Dev ARCI funding is $50,000 of cash with $25,000 of in-kind services donated by Nature Works for this funding. Activities must be completed by March 31, 2012.
The deadline to start was originally March 31, 2011 but has been extended to June. But we want to get started asap.
Nature Works and Kinross are also partners under my NSERC IE grant and are eligible for support under it.
Problem is that Fed Dev has been extremely clear that we cannot use funding from other projects to extend or enhance what their funding is doing in any way.
If we do use funding to top up the Fed Dev project and they catch us they'll remove that amount from the Fed Dev award.
We have to have a discrete $50,000 project. After it is finished we can do what we like for a different project under a different name.
Other issues - I cannot send any funding from my NSERC grant to universities. So I would have to employ any labour directly from Fleming.
Fleming is unionized and our pay rates are higher so this is a disincentive if the budget is tight.
What we need to do is find a 3-6 month discrete project that costs $50,000 (with in-kind services provided by Nature Works so we can claim the funding).
ed by March 31, 2012.
in any way.
Nature Works Remediation Project FedDev 846236
Labour
Flemng Lab Staff 10,076.00
Uof G Labour 7,956.00
Trent- sampling by Trent personnel / 211.00
training of Fleming personnel
Trent - Sample prep 7,072.00
Trent - stability test (2 analyses: same 1,481.00
day and one week)
Trent - 6 sets of analyses (e.g. 4,442.00
monthly or spaced unevenly)
Trent - 6 SeqEx steps @ $ 650 each 2,538.00
Trent- analysis of extracts from 1,000.00
reactor samples, TAs and As
speciation
Total Labour 34,776.00
Materials
Fleming Lab Materials 6,409.00
UofG Lab Materials 750.00
Trent - stability test (2 analyses: same 1,119.00
day and one week)
Trent - 6 sets of analyses (e.g. 3,358.00
monthly or spaced unevenly)
Trent - 6 SeqEx steps @ $ 650 each 1,362.00
Trent- sampling by Trent personnel / 189.00
training of Fleming personnel
Total Materials 13,187.00
Sub Contracts
Car Rental UofG 330.00
Lab Services UofG 500.00
Imaging Facilities UofG 1,300.00
Total Sub Contracts 2,130.00
Total 50,093.00
