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字型不統一 格式錯誤多 卷數不見了，頁碼不對 斜體不當 1-5 篇順序不對 1. Nordstrom, Jessica J. L.; R. Rangdale, Rachel; M. C. L. Vickery, Michael C L; Formatted: Indent: Left: 0", Hanging: 1.5 ch, First line: -1.5 ch A. M.B. Phillips, M. B. Andrea M B;, S. L. Murray, Shelley L; S. Wagley, Sariqa; and A. DePaola, Angelo. 2006. Evaluation of an alkaline Formatted: Font: Not Bold Formatted: Font: Not Italic phosphatase-labeled oligonucleotide probe for the detection and Formatted: Font: Not Italic enumeration of the thermostable-related hemolysin (trh) gene of Vibrio Formatted: Font: Not Italic parahaemolyticus. Journal of. F food protectionProt Vol:. 0362-028X Formatted: Font: Italic (怎麼每一篇頁碼都一樣，都一樣的奇怪) Formatted: Font: Not Italic Formatted: Font: Italic Reliable methods are needed to detect total and pathogenic Vibrio parahaemolyticus. One marker Formatted: Font: of V. parahaemolyticus virulence is the thermostable-related hemolysin. We developed an alkaline phosphatase-labeled DNA probe method for the specific detection and enumeration of trh-positive V. parahaemolyticus by colony hybridization. The probe was tested against a panel of 200 bacterial strains and determined to be specific for trh-positive V. parahaemolyticus. Additionally, the trh alkaline phosphatase probe colony hybridization was successfully used to detect and enumerate trh-positive V. parahaemolyticus in seafood and water samples collected from the United States and the United Kingdom. 2. Goto, M; Takahashi, H; Segawa, Y; Hayashidani, H; Takatori, K; Hara-Kudo, Y. 2007. Real-time PCR method for quantification of Staphylococcus aureus in milk. Journal of food protection. 0362-028X A reproducible real-time PCR method that targets the putative transcriptional regulator gene of Staphylococcus aureus was developed to quantify this microorganism in milk samples. On the basis of partial sequences of this gene determined from S. aureus strains, we designed the specific primers and probe for use in a quantitative PCR assay. These specificities were confirmed with 25 strains of S. aureus and 35 strains of other bacteria. A real-time PCR assay with serial 10-fold dilutions of purified DNA and pure culture was conducted. It was possible to construct standard curves with a high correlation coefficient (r2 = 0.99) in the range of 50 ng to 50 fg for purified DNA and 10(7) to 10(1) CFU/ml for a pure culture. The constructed standard curve for milk samples was similar to that for the pure culture, and the quantification of S. aureus in the range of 10(7) to 10(1) CFU/ml was possible. Moreover, to determine how our real-time PCR method would perform under actual analytical conditions, we quantified the DNA from S. aureus after two types of heat treatments were used for the pasteurization of milk. The amount of DNA found was affected after heat treatment at 63 degrees C for 30 min (low-temperature long-time method) but not at 72 degrees C for 15 s (high-temperature short-time method). The results indicate that the real-time PCR method developed in this study is effective for monitoring S. aureus contamination in milk because of its high specificity and sensitivity. 3. Ngundi, Miriam M; Shriver-Lake, Lisa C; Moore, Martin H; Ligler, Frances S; Taitt, Chris R. 2006. Multiplexed detection of mycotoxins in foods with a regenerable array. 期刊不見了 0362-028X The occurrence of different mycotoxins in cereal products calls for the development of a rapid, sensitive, and reliable detection method that is capable of analyzing samples for multiple toxins simultaneously. In this study, we report the development and application of a multiplexed competitive assay for the simultaneous detection of ochratoxin A (OTA) and deoxynivalenol (DON) in spiked barley, cornmeal, and wheat, as well as in naturally contaminated maize samples. Fluoroimmunoassays were performed with the Naval Research Laboratory array biosensor, by both a manual and an automated version of the system. This system employs evanescent-wave fluorescence excitation to probe binding events as they occur on the surface of a waveguide. Methanolic extracts of the samples were diluted threefold with buffer containing a mixture of fluorescent antibodies and were then passed over the arrays of mycotoxins immobilized on a waveguide. Fluorescent signals of the surface-bound antibody-antigen complexes decreased with increasing concentrations of free mycotoxins in the extract. After sample analysis was completed, surfaces were regenerated with 6 M guanidine hydrochloride in 50 mM glycine, pH 2.0. The limits of detection determined by the manual biosensor system were as follows: 1, 180, and 65 ng/g for DON and 1, 60, and 85 ng/g for OTA in cornmeal, wheat, and barley, respectively. The limits of detection in cornmeal determined with the automated array biosensor were 15 and 150 ng/g for OTA and DON, respectively. 4. Leggate, Johanna; Blais, Burton W. 2006. An internal amplification control system based on primer-dimer formation for PCR product detection by DNA hybridization. Journal of food protection . 0362-028X The detection of PCR products by DNA hybridization techniques can suffer from inhibition of the amplification process by sample matrix components. We have designed a simple internal control system for PCR based on the incorporation of a primer pair with complementary 3' ends, resulting in the generation of a unique "primer-dimer" detectable by hybridization with a specific capture probe immobilized on polyester cloth as part of an array of amplicon-specific probes. The inclusion of this primer pair did not adversely affect the amplification and subsequent detection of target gene sequences by hybridization with immobilized probes in either single gene amplification or multiplex PCR systems. The failure to amplify target gene sequences because of the presence of inhibitors was mirrored by a failure to amplify the internal control primer-dimer, demonstrating the efficacy of this system in identifying the presence of DNA amplification inhibitors. 5. Wan, Kai; Yousef, Ahmed E; Schwartz, Steve J; Wang, Hua H. 2006. Rapid, Specific, and Sensitive Detection of Spoilage Molds in Orange Juice Using a Real-Time Taqman PCR Assay. Journal of Food Protection. 0362-028X The outgrowth of spoilage organisms, including molds and yeasts, results in significant financial loss to the food industry and wastes natural resources. The objective of this study was to develop a rapid, specific, and sensitive real-time PCR method for detecting spoilage molds during screening of raw materials and final product quality control analysis. The 18S rRNA gene was used to develop PCR primers and probe. With this set of primers and probe, less than 1,000 mold cells per milliliter of orange juice (10 cells per reaction) were detected with the real-time PCR system within 6 to 7 h. No cross-reactivity was found with other common foodborne bacteria, yeasts, or food ingredients. This technique is significantly faster than current detection and identification procedures, which take from days to weeks.
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