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Correlation of Wnt Signaling Activity in Lung Cancer and Tobacco

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Correlation of Wnt Signaling Activity in Lung Cancer and Tobacco Powered By Docstoc
					No.       FP00704057
Title     Correlation of Wnt Signaling Activity in Lung Cancer and Tobacco
          Smoking
Period    2005-2007
Summary   Purpose     We previously reported that the role of Wnt signaling in
          mesothelioma. We evaluated the significance of Wnt signaling in
          non-small cell lung cancer (NSCLC).
          Methods       Dvl expression in NSCLC was analyzed using Western blot
          analysis. To evaluate the significance of Wnt signaling in NSCLC, Dvl
          expression was suppressed using small interfering RNA (siRNA) of Dvl.
          First, mesothelioma cell lines with Dvl overexpression were transfected
          with siRNA of Dvl, and were examined cell growth and colony
          formation. Next, NSCLC cell lines were transfected with siRNA of Dvl,
          and were examined cell growth.
          Results   Dvl-3 was overexpressed in 75% of NSCLC fresh tumors, and
          58% of NSCL cell lines. siRNA of Dvl-3 downregulated Dvl-3
          expression in 3 mesothelioma cell lines, and caused suppression of cell
          growth and colony formation. Five of 8 NSCLC cell lines transfected
          with siRNA of Dvl-3 showed suppression of cell growth.
          Conclusion Our data suggest that Wnt signaling is activated through
          Dvl overexpression in NSCLC. Inhibition of this signaling led to
          significant    anti-tumor   effects.   These   results   demonstrate   Dvl
          overexpression in human cancer and, specifically, that Wnt signaling
          plays a role in non-small cell lung pathogenesis. These data offer
          possible new avenues for therapeutic intervention.

				
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posted:8/25/2012
language:English
pages:1