Title Correlation of Wnt Signaling Activity in Lung Cancer and Tobacco
Summary Purpose We previously reported that the role of Wnt signaling in
mesothelioma. We evaluated the significance of Wnt signaling in
non-small cell lung cancer (NSCLC).
Methods Dvl expression in NSCLC was analyzed using Western blot
analysis. To evaluate the significance of Wnt signaling in NSCLC, Dvl
expression was suppressed using small interfering RNA (siRNA) of Dvl.
First, mesothelioma cell lines with Dvl overexpression were transfected
with siRNA of Dvl, and were examined cell growth and colony
formation. Next, NSCLC cell lines were transfected with siRNA of Dvl,
and were examined cell growth.
Results Dvl-3 was overexpressed in 75% of NSCLC fresh tumors, and
58% of NSCL cell lines. siRNA of Dvl-3 downregulated Dvl-3
expression in 3 mesothelioma cell lines, and caused suppression of cell
growth and colony formation. Five of 8 NSCLC cell lines transfected
with siRNA of Dvl-3 showed suppression of cell growth.
Conclusion Our data suggest that Wnt signaling is activated through
Dvl overexpression in NSCLC. Inhibition of this signaling led to
significant anti-tumor effects. These results demonstrate Dvl
overexpression in human cancer and, specifically, that Wnt signaling
plays a role in non-small cell lung pathogenesis. These data offer
possible new avenues for therapeutic intervention.