Table2

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					Table 2. A catalog towards the full-length L. obliqua cDNAs coding for proteins probably involved in envenomation.
                    Cl               Seq      SigP    Cleavg          Mature          Match to NR protein                    Full-
Sequence name1            GenBank3                             MW 6            pI8                             E val10                                  Comments               Tissue12
                   no.2              size4   Result    Pos.5           MW 7               database9                        length11
Cysteine proteinases
LOqua-CysPep1      423    AY829805    65      IND              7.08                                           [Helicoverp N
                                                                               6.92 cathepsin L-like protease 5E-026                  cysteine proteinase, cathepsin-like         T
LOqua-CysPep2      55     AY829838   338      SIG     20-21    36.9   34.773                                  1E-172
                                                                               5.73 cathepsin B-like cysteine proteinase [ Y          cysteine proteinase, cathepsin-like         B
LOqua-Cyst1        125    AY829806   120      SIG     22-23    13.3   10.59                                   6E-004
                                                                               5.09 L-cystatin precursor >gnl|BL_OR           Y       cystatin                                    T
Defense proteins
LOqua-Def2         430    AY829737   121     CYT               13.3                                          2E-020
                                                                               9.4 Attacin A precursor >gnl|BL_ORD_           N       attacin                                     T
LOqua-Def3         51     AY829840   233      SIG     17-18    25.1   22.968   5.38 Attacin E precursor (Immune prote
                                                                                                            1E-107            Y       attacin precursor                           B
LOqua-Def4         21     AY829848    65      SIG     25-26    7.23   4.255    10.9 antimicrobial peptide cecropin 6 [Man Y
                                                                                                              5E-007                  cecropin                                    B
LOqua-Def6         36     AY829857    75      SIG     20-21    8.23   5.786                                  4E-004
                                                                               9.15 gallerimycin [Spodoptera frugiperda] Y            antimicrobial toxin                         B
LOqua-Ease1        454    AY829807    63     CYT               7.61                                          4E-019
                                                                               6.04 Esterase-like protein (ESR-LP) [BombN             esterase                                    T
Lectins
LOqua-Lect1        120    AY829822   321      SIG     19-20    35.8   33.653                                 9E-072
                                                                               6.05 lipopolysaccharide binding protein [B Y           Immulectin                                  T
LOqua-Lect3        140    AY829836   320      SIG     20-21    36.3   33.922   6.28 lipopolysaccharide binding protein [B Y
                                                                                                             2E-073                   lectin                                      B
LOqua-Lect4        184    AY829849   148     CYT               17.2                                          6E-077       N
                                                                               4.91 ENSANGP00000012234 [Anopheles gambi c-type lectin                                             B
LOqua-Lect5        33     AY829846   162      SIG     16-17    18.3   16.299   5.61 lipopolysaccharide binding protein [B Y
                                                                                                             5E-010         lectin                                                B
Lipocalins
LOqua-Lipcl1        1     AY829833   201      SIG     16-17    22.4   20.558                                    2E-052
                                                                               6.11 biliverdin binding protein-II [Samia      Y       lipocalin                                   B
LOqua-Lipcl3       104    AY829856   137      SIG     16-17    15.4   13.493                                 2E-007    Y
                                                                               4.92 odorant-binding protein AgamOBP26 [An             lipocalin                                   B
LOqua-Lipcl4       37     AY829809   198     CYT               21.6                                             5E-050
                                                                               6.12 biliverdin binding protein-II [Samia      Y       lipocalin                                   T
Serine proteases
LOqua-PPOAF1       264    AY829844   398      IND              42.9                                         1E-168      N
                                                                               5.16 masquerade-like serine proteinase hom             serine protease, PPO-activating factor      B
LOqua-SP1          220    AY829818   519      SIG     19-20    57.4   55.244                                   proteinase Y
                                                                               6.34 pattern recognition serine 1E-128                 serine protease                             T
LOqua-SP2          333    AY829819   322     CYT               36.1                                    4E-042      Y
                                                                               6.53 ENSANGP00000010929 [Anopheles gambi serine protease                                           T
LOqua-SP3          396    AY829820   272     CYT               29.7                                         2E-068
                                                                               8.18 30kP protease A (43k peptide) precursY            serine protease, chymotrypsin-like          T
LOqua-SP4          118    AY829821   229     CYT               25.9                                            1E-030
                                                                               5.88 trypsin-like serine protease [Ctenocep N          serine protease, PPO-activating factor      T
LOqua-SP5          109    AY829842   242      IND              26.8                                    7E-043      N
                                                                               5.85 ENSANGP00000020324 [Anopheles gambi serine protease, PPO-activating factor                    B
LOqua-SP6          12     AY829843   302      SIG     34-35    34.1   30.136                          2E-047      Y
                                                                               4.9 ENSANGP00000006359 [Anopheles gambi serine-protease, plasminogen activator                     B
LOqua-SP7          130    AY829841   280     CYT               30.5                                            3E-076
                                                                               6.09 trypsin-like serine protease [Ctenocep N          serine-protease, plasminogen activator      B
Serpins
LOqua-Serp1        130    AY829814   476      SIG     18-19    52.4   50.199   5.13 serpin-2 [Bombyx mori]     1E-152         Y   serpin
                                                                                                                             539 e-152                                            T
LOqua-Serp2        131    AY829815   395      SIG     18-19    43.8   41.601   5.07 serpin-2 [Bombyx mori]     1E-153         Y   serpin
                                                                                                                             541 e-153                                            T
LOqua-Serp3        42     AY829816   383     CYT               43.2            6.09 serpin 1                        461 e-128 serpin
                                                                                                               1E-128    N                                                        T
LOqua-Serp4        43     AY829817   322      IND              36.4            6.69 serpin 1                        399 e-110 serpin
                                                                                                               1E-110    N                                                        T
LOqua-Serp6        247    AY829847                                                                                            Y       serpin                                      T
                   Cl                 Seq      SigP    Cleavg              Mature          Match to NR protein                   Full-
Sequence name1           GenBank3                               MW 6                pI8                             E val10                                Comments       Tissue12
                  no.2                size4   Result    Pos.5               MW 7               database9                       length11
Other protease inhibitors
LOqua-PI1          317   AY829810      154     SIG     19-20        17     14.644                                3E-011             3e-011
                                                                                    6.02 similar to CG8806-PA [Apis mellifera] Y 69 protease inhibitor                       T
LOqua-PI3          374   AY829811      398     SIG     21-22        44.4   41.753                          2E-015      Y
                                                                                    8.7 ENSANGP00000010965 [Anopheles gambi trypsin inhibitor-like                           T
LOqua-PI4          86    AY829812      102     SIG     20-21        11     8.583                                   0.005
                                                                                    5.55 predicted CDS, trypsin inhibitor-li      Y       trypsin inhibitor-like             T
LOqua-PI5          420   AY829813       86     SIG     19-20        9.7    7.268     5                           0.035     N
                                                                                          CG33196-PB [Drosophila melanogaster             trypsin inhibitor-like             T
LOqua-PI6          252   AY829839       86     SIG     19-20        9.31   7.157                                  0.002     Y
                                                                                    7.71 Hypothetical protein CBG19175 [Caeno             trypsin-inhibitor like             B
LOqua-PI7          333   AY829835       94     SIG     18-19        10.2   7.962                                   4E-028
                                                                                    8.31 kazal-type proteinase inhibitor [Mandu Y         kazal-type protease inhibitor      B
LOqua-PI9          49    AY829837       56     SIG     17-18        6.1    4.057                                    0.002       Y
                                                                                    9.02 plant defensin precursor [Vigna radiata]          0.002
                                                                                                                                      43 proteinase inhibitor                B
Phospholipase A2
LOqua-PLA1         154   AY829845      107     SIG     22-23        12.3   9.593                              2E-010       >g
                                                                                    6.9 RE08605p [Drosophila melanogaster] Y              PLA2                               B

1                                                               7
 Putative toxins                                                 Molecular mass of the mature protein
2                                                               8
 Cluster numbers as in Table 1                                   pI of the mature protein
3                                                               9'
 GenBank accession number                                          Best protein match by blastX to the non-redundant protein database of NCBI
4                                                               10
 Length of aminoacid sequence                                      E-value, significance of match to NR sequence of previous column
5                                                               11
 Signal peptide cleavage position                                  Complete sequence (Y) or truncated (N)
6                                                               12
 Molecular mass before removal of signal peptide                   Tissue used for cDNA library construction: B, bristles; T, tegument

				
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