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					                       Cell Biology Laboratory (BIO 440L)

                               Fall 2008 Schedule

Amit Singh, Assistant Professor, SC 303C, phone x.92894
Email: amit.singh@notes.udayton.edu


Meghana Tare, Teaching Assistant (TA)
Email: taremegh@notes.udayton.edu
Office hours: Thursday 4.00-5.00 PM SC345/347

Lab Time
Tuesday 1:30-4:20pm

Location
Advanced Biology teaching lab

Handouts
Handouts will be distributed. You are expected to read them carefully. Quizzes will be
given at the beginning of each class on the previous class material. Quizzes questions will
also cover the reading assignments.

Binder and Notebook
A 3-ring binder will be necessary to keep your handouts. A college notebook (quad ruled)
will serve as a laboratory notebook. Those 2 items are required. The lab notebook should
record the design and goals of a particular experiment, your observations, results
obtained, questions and speculations. The lab notebook is used to prepare your lab
reports.

Laboratory reports
You will prepare a lab report at the conclusion of each module. Please see guidelines
when preparing your lab reports (handed out separately).

Attendance
Attendance is mandatory. If you fail to show for class, you will receive a zero for the day.
If you are sick, a note from the health center or letter from the Dean must be turned in to
me.

Lab cleanliness
You must clean up after each experiment and be careful not to leave a mess around your
lab area or shared areas of the lab.
Lab participation
All labs have activities and experiments you will be expected to perform and carefully
observe. Please ask questions! You will learn only if you challenge yourself and your
instructors. This portion of your grade will be based on your attitude toward the lab. We
expect you to:

1)      be prepared: read the handout over well and know what you are doing

2)      be cooperative, both with your instructors and with the other students

3)      be enthusiastic: lab work is what science is all about, so you should have fun with
        it. We ask you to care about the lab, not to act as if you would rather be
        somewhere else. Your attitude is important to how much you can learn.

Grading
Attendence, Lab conduct                                                           5%
Laboratory reports                                                                25%
Troubleshooting                                                                  5%
Quizzes                                                                          40%
Final exam                                                                       25%


 Grade            Cut-off
   A               >93.00
  A-          90.00-92.99
  B+          86.00-89.99
   B          83.00-85.99
  B-          80.00-82.99
  C+          76.00-79.99
   C          73.00-75.99
  C-          70.00-72.99
  D           60.00-69.99
   F               <60.00


                                 Disability Statement
1. “University of Dayton students wishing to request a disability related accommodation must
submit the request in writing to the University’s Learning Enhancement and Academic
Development (LEAD) Office, Attention: Disability Services (937) 229-2066 located in the Ryan C.
Harris Learning Teaching Center, LTC 023. If you have secured a current Self-Identification Form
indicating you have a disability which requires academic accommodations, please present the
Self-Identification Form to me so I will be able to provide the appropriate accommodation needed
in this class.”

2. “Students with disabilities who believe that they may need accommodations in this class are
encouraged to contact the LEAD: Disability Services at (937) 229-2066, as soon as possible to
better ensure that such accommodations are implemented in a timely manner. If you have
secured a current Self-Identification Form indicating you have a disability which requires academic
accommodations, please present the Self-Identification Form to me so I will be able to provide the
appropriate accommodation needed in this class.”

3. “If you need course adaptations or accommodations because of a disability, please see the
LEAD: Disability Services at (937) 229-2066, LTC 023. If you have secured a current Self-
Identification Form indicating you have a disability which requires academic accommodations,
please present the Self-Identification Form to me so I will be able to provide the appropriate
accommodation needed in this class.”




                                 2008 FALL SCHEDULE

Date           Lab                Topics (all diagnostic labs are simulations)


8/26             1               Introduction. The lab notebook. Lab organization and
                                 cleanliness. How to dispose of biohazardous material. How
                                 to pipette correctly.

                                 MODULE 1: Cell structure and function

9/2              2               Labelling cells and subcellular organelle using
                                 Fluorescent probes and visualizing them by epifluorescence
                                 microscopy.
                                 Visualization of proteins (actin filaments) in fluorescence
                                 microscopy.
                                 Readings: handout and reading assignment.

9/9              3               Cell death using Acridine orange staining and in situ cell
                                 Death Kit by using confocal miscroscope.
                                 Readings: Handouts

9/16             4               Cell proliferation using BrDU staining and
                                 immunohistochemistry

9/23             5               Scanning electron Microscopy to study morphology of the
                                 Tissue (HRSEM, Nest facility)
                             Module 2 Protein chemistry

9/30           6            Isolation of proteins from tissue and protein electrophoresis
                             Readings: handout and reading assignment.


10/7           7           Western blotting: detection of serum albumin.
                           Readings: handout and reading assignment,
        Lab report 1 due (Module 1)

10/14         8A             TROUBLESHOOTING LAB: Use your resources,
                             follow the study guide and carry out the experiment.
                             You will be provided with a kit and need to finish the
                             experiment on your own.
                             ELISA: diagnosis of Epstein-Barr virus (EBV),
                             responsible for infectious mononucleosis.
                             Readings: handout and reading assignment, pp 732.

                8B           Mad Cow Disease
                             Readings: handout

10/21               9        RT-PCR: a model for the molecular biology of HIV
                             replication.
                             Readings: handout and reading assignment, pp 115-117
                             and 732-733.



Date            Lab            Topics (all diagnostic labs are simulations)


                             MODULE 3: DNA-BASED DIAGNOSTIC TESTING

10/28          10            Separation of DNA & RNA by gel filtration
                             chromatography
                             Readings: handout
                             Lab report 2 due (Module 2)

3/29           11            Visualizing cancer cells and detection of a cancer gene
                             (p53 mutation).
                             Readings: handout, reading assignment, and reading
                             assignment p 750.

11/3           12            Clinical cholesterol test and genetic screening of
                             hypercholesterolemia
             Readings: handout, reading assignment.

11/10   13   Final Exam
             Lab report 3 due (Module 3)

				
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