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QuickFacts_Transgenic

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					Service                Rate (U-M      Approx. Turnaround
                       Units)         Time
B6SJLF2 Hybrid                 $3,000
Transgenic Mouse
Production




C57BL/6J Transgenic           $5,400
Mouse Production




SD Outbred                    $7,500
Transgenic Rat
Production




F344/NHsd                    $10,100
Transgenic Rat
Production




ES Cell                       $4,100
Electroporation
Transient Cre or FLP            $820
ES Cell
Electroporation
ES Clone Expansion     $530 per clone
KOMP or Genetrap                $530
ES Clone Expansion
Blastocyst               $2,000
Microinjection of
C57BL/6 ES Cells
Blastocyst               $1,900
Microinjection of
129 Strain ES Cells
De Novo ES Cell Line     $2,100
Derivation




Rederivation of            $810
Pathogen Free
Mouse Lines
Mouse Speed Cryo           $900
Mouse 8-Cell Egg       $900 per
Cryopreservation         session
Mouse Strain Cryo-         $790
recovery
Mouse Sperm Cryo           $620
BAC Recombineering   $2,000
Notes

Three transgenic mice are guaranteed.
Mice are produced in the (C57BL/6 X
SJL)F2 genetic background. Other genetic
backgrounds may be specified by
investigators (additional cost). Large
DNAs (BACs, YACs, P1s) may be specified
at no additional cost.
Three transgenic mice are guaranteed.
Mice are produced in the (C57BL/6 X
SJL)F2 genetic background. Other genetic
backgrounds may be specified by
investigators (additional cost). Large
DNAs (BACs, YACs, P1s) may be specified
at no additional cost.
Three transgenic rats are guaranteed.
Rats are produced in the Sprague-
Dawley genetic background (Crl:CD
(SD)IGS BR ). Other genetic backgrounds
may be specified by investigators
(additional cost). Large DNAs (BACs,
YACs, P1s) may be specified at no
additional cost.
Three transgenic rats are guaranteed.
Rats are produced in the Sprague-
Dawley genetic background (Crl:CD
(SD)IGS BR ). Other genetic backgrounds
may be specified by investigators
(additional cost). Large DNAs (BACs,
YACs, P1s) may be specified at no
additional cost.
Preparation of ES cell lines from mouse
blastocysts. Preparation of ES cell lines
from mouse blastocysts. Preparation of
ES cell lines from mouse blastocysts.We
will collect blastocysts from your
genetically engineered mouse model and
culture them under conditions that are
conducive to establishing ES cell lines.
The number of ES cell lines produced is
propotional to the number blastoycsts
available for culture. The success of the
Transgenic Core in de novo ES cell line
derivation meets or exceeds published
standards.
Acquistion of BACs from BAC library
resources. Purification of BAC DNA
suitable for pronuclear microinjection
(transgenic mice or transgenic rats).
Pulsed field gel mapping of BAC DNA to
confirm identiy of BAC. Genetic
modification of BACs by recombineering
approaches to produce gene knockins,
knockouts. Prepareation of gene
targeting vectors for the modifcation of
genes in embyronic stem cells.
Discuss structure of the desired BAC with
the investigator and propose a
recombineering path. Plasmids for
knockins of fluorescent reporter
molecules and Cre recombinase are
available.

				
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posted:8/15/2012
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