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HyNicOligonucleotideColorimetricMSRAssayCalculator

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HyNicOligonucleotideColorimetricMSRAssayCalculator Powered By Docstoc
					HyNic-Oligonucleotide Molar Substitution Ratio (M


    HyNic MSR Calculator
                                                                     Insert required
    Oligonucleotide Name                                   Oligo 1
                                                                     information in the
    Molar extinction coefficient of oligo (liter/mol-cm)   385400    light green input
    Concentration of Oligonucleotide (OD260/µL)             0.45     fields
    Oligo volume in Assay (µL)                                2
    2-SBA Reagent Volume (µL)                                18      The calculator
    A345 Reading (1-cm cuvette pathlength)                   2.8     output will be
    Molar Substitution Ratio (MSR)                          0.84     displayed in the
                                                                     blue output fields
                                                                     in bold.
                      Version # 03.20.12




ution Ratio (MSR) Calculator


 Insert required
 information in the
 light green input



 The calculator
 output will be
 displayed in the
 blue output fields
 in bold.

				
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