Cell Cycle and Mitosis Pre-Lab Pre-Lab is on your OWN PAPER: Whale p. 614-615 and Owl p. 588-589 1. In a plant, where are cells “continually dividing?” 2. Sketch a root and label. Please include the following parts: root cap, apical meristem, root hairs, xylem and phloem. 3. From where in the root are new cells produced? Cell Cycle Lab Objective: To determine the relative number of cells undergoing the different stages of the cell cycle. Problem IV, DV, Control, Constants (Controlled Introduction Variables) Hypothesis Quantitative Data Table: # Group Group Group Group Group Group Group Group Group Group Group Group Class cells 1 2 3 4 5 6 7 8 9 10 11 12 in: Average I P M A T Qualitative Data Table: Stage Appearance Interphase Prophase Metaphase Anaphase Telophase Graph: Make an appropriate graph of the class average – showing the % of cells in each stage. Analysis: 1. Which phase of the cell cycle had the most cells? 2. Why do you think the phase to question #1 had the most cells? 3. If your data had many cells undergoing mitosis, to which part of an onion root do you think your cells belong? Why? 4. If your data had a few cells undergoing mitosis, to which part of an onion root do you think your cells belong? Why? Conclusion: Mitosis Lab Data Table A: Relative Duration of Each Phase of Mitosis Phase of Mitosis Tally Marks Total Count Percentage Time in minutes Prophase Metaphase Anaphase Telophase Data Table B: Data Collected by the Entire Class Phase of Mitosis Total Count Percentage Time in minutes Prophase Metaphase Anaphase Telophase GRAPH: Create an appropriate graph of your data vs. the class data. Answer: ON YOUR OWN PAPER 1. What color are the chromosomes? Why? 2. How can you distinguish between early and late anaphase? 3. According to your data table, which phase of mitosis lasts the longest? 4. Why might this phase (from #3) require more time than other phases? 5. According to your data table, which phase takes the least amount of time? 6. How do your results compare with those of the entire class? 7. In this investigation, you assumed the percentage of the total time that any given phase takes is equal to the percentage of cells in that phase at any moment. Why might this not be true for very small samples of cells?
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