Mitosis-and-Cell-Cycle-Lab by hedongchenchen

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									Cell Cycle and Mitosis Pre-Lab
Pre-Lab is on your OWN PAPER:                     Whale p. 614-615 and Owl p. 588-589

      1. In a plant, where are cells “continually dividing?”
      2. Sketch a root and label. Please include the following parts: root cap, apical meristem, root hairs, xylem
         and phloem.
      3. From where in the root are new cells produced?

                                                  Cell Cycle Lab
Objective: To determine the relative number of cells undergoing the different stages of the cell cycle.

          Problem                                                       IV, DV, Control, Constants (Controlled
          Introduction                                                   Variables)
          Hypothesis


Quantitative Data Table:

  #       Group   Group   Group   Group   Group   Group   Group   Group   Group   Group   Group   Group    Class
cells       1       2       3       4       5       6       7       8       9      10      11      12
 in:                                                                                                      Average

  I

 P

 M

 A

 T




Qualitative Data Table:

Stage                       Appearance

          Interphase

          Prophase

          Metaphase

          Anaphase

          Telophase



Graph: Make an appropriate graph of the class average – showing the % of cells in each stage.
Analysis:

         1. Which phase of the cell cycle had the most cells?
         2. Why do you think the phase to question #1 had the most cells?
         3. If your data had many cells undergoing mitosis, to which part of an onion root do you think your cells
            belong? Why?
         4. If your data had a few cells undergoing mitosis, to which part of an onion root do you think your cells
            belong? Why?


Conclusion:

                                                    Mitosis Lab
Data Table A: Relative Duration of Each Phase of Mitosis

Phase of Mitosis     Tally Marks                                       Total Count    Percentage     Time in minutes
Prophase
Metaphase
Anaphase
Telophase



Data Table B: Data Collected by the Entire Class

Phase of Mitosis                Total Count                    Percentage                      Time in minutes
Prophase
Metaphase
Anaphase
Telophase



GRAPH: Create an appropriate graph of your data vs. the class data.

Answer: ON YOUR OWN PAPER

    1.   What color are the chromosomes? Why?
    2.   How can you distinguish between early and late anaphase?
    3.   According to your data table, which phase of mitosis lasts the longest?
    4.   Why might this phase (from #3) require more time than other phases?
    5.   According to your data table, which phase takes the least amount of time?
    6.   How do your results compare with those of the entire class?
    7.   In this investigation, you assumed the percentage of the total time that any given phase takes is equal to the
         percentage of cells in that phase at any moment. Why might this not be true for very small samples of cells?

								
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