NEGATIVE ASSOCIATION BETWEEN LEPTIN AND aBMD and leptin, when controlling for lean body mass (r=-
BONE MINERAL DENSITY IN HUMAN MALES. 0.22, p<0.05), for MVC (r=-0.23, p<0.05) and for lean body
Delgado-Guerra Saﬁra 1 , Olmedillas Hugo 1 , Guadalupe mass and MVC (r=-0.24, p<0.05).
Amelia 1 , Pérez-Gómez Jorge, Ara Ignacio 2 , Vicente- Conclusion.
Rodríguez Germán 2 , Sanchís-Moysi Joaquín, Dorado This study indicates that leptin may exert a negative inﬂu-
Cecilia 1 , Calbet Jal 1 ence on bone mineral density in males.
(University of Las Palmas de Gran Canaria 1 , University of References.
Zaragoza 2 , Spain) Lorentzon et al. (2006) J Bone Miner Res. 21(12): 1871-8.
Ducy et al. (2000) Cell 100(2) : 197-207
Introduction. This study was supported by Ministerio de Educación y
Body weight is positively associated with areal bone min- Ciencia (BFI2003-09638, BFU2006-13784) the Gobierno
eral density (aBMD) in males and females, and negatively de Canarias (PI042005/177), and the Canary Islands Po-
associated with osteoporosis and fracture risk. In fact, obe- lice Academy.
sity is associated with lower risk of osteoporosis. However, Keywords: Leptin, Bone Mineral Density
it remains unknown if the association is mediated exclu-
sively by the mechanical stimulation elicited by the extra-
weight caused by adipose tissue mass or if it is generated
by adipokines or a circulating factors capable of promoting
osteogenesis. Cross-sectional studies on the relationship
between serum leptin concentration and aBMD have yield
discordant results (Lorentzon et al. 2006). Moreover, leptin
deﬁcient mice have a high bone mass phenotype that may
be corrected by ICV leptin injection, while the leptin recep-
tor deﬁcient mice have a low bone mass proﬁle resistant to
leptin administration (Ducy et al. 2000). Based on these
studies, a negative correlation between leptin and aBMD
is expectable. However, sustained elevated concentrations
of serum leptin cause leptin resistance, and bone mass is
also inﬂuenced by muscle mass and physical activity.
In this context, we decided to explore which is the relation-
ship between leptin and bone mass in adult human male
accounting for the impact that some confounding variables
may have on this relationship such as: lean body mass and
One hundred ﬁfty-nine males (age=29.0±6.8, body
fat=18.1±7.2, total bone mineral density=1.25±0.08
g/cm2, mean±sd) participated in this investigation. aBMD
of the total body, lumbar spine, femoral neck, hip, and legs,
as well as total body adipose tissue and lean mass were
measured by using DXA. The concentrations in serum lep-
tin were determined with ELISA commercial kits in blood
samples obtained under fasting conditions. The maximal
leg extension voluntary contraction (MVC) in the squat po-
sition with knees bent at 90o was assessed with a force
Leptin correlated with body mass, fat mass, BMI, the per-
centage of body fat and femoral neck aBMD (r=0.48, 0.72,
0.53, 0.72, -0.21, respectively, all p<0.05). A statistically
signiﬁcant bivariate correlation between leptin and femoral
neck aBMD (r=-0.21, p<0.05) was observed. There was
a negative partial correlation between whole body aBMD
and leptin concentration when controlling for MVC (r=-0.19,
p<0.05), for lean body mass (r=-0.17, p<0.05) and for lean
body mass and MVC (r=-0.21, p<0.05). Similar results
were obtained for the relationship between femoral neck
12th Annual Congress of the ECSS, 11–14 July 2007, Jyväskylä, Finland