Animal Hazardous Agents Form – Part II Date last revised:
It is the Principal Investigator’s responsibility to post the final version of
this form on the lab/animal room door two weeks prior to start of protocol.
Department: Protocol #:
Authorized Personnel Pager/Mobile
Precautions for this protocol (for assistance, contact OEH&S and/or DLAR)
Type of Hazard
[ ] BSL 1:
[ ] BSL 2:
Check category and list agent(s)
[ ] human cell lines or human tumors*:
Location of Biosafety Cabinet
Chemical Agents (including gas anesthetics)**: Complete Freund’s Adjuvant (Freund’s Complete Adjuvant)
How long should precautions be followed? [x] 1 week (treat bedding as hazardous for 7 days)
(Note: You must notify DLAR each time these precautions [ ] Duration of project
should be instituted) [x] Other:
[x] gloves [x] dust mask [ ] N95
[ ] foot covers [ ] head cover/ bonnet [ ] safety
Protective Clothing: [ ] goggles [x] sleeves (while working in biological safety
cabinet – for personal protection and maintains sterile
[x] for lab/ research staff: gown or lab coat
[ ] other:
[ ] urine [ ] feces [ ] saliva
Route of Animal Excretion: [ ] respiratory [x] none expected
[ ] other:____________________
[x] routine incineration [x] chemical waste
[ ] biohazardous waste [ ] radioactive waste
Animal Carcass Disposal:
[x] other: carcasses stored in biohazard bag prior to
[ ] regular waste
Biohazardous Caging/Bedding Disposal:
[ ] autoclave then treat as regular waste
[ ] regular waste [X] chemotherapeutic/chemical
[ ] radioactive decay [ ] radioactive waste
Chemical or Radioactive Caging/Bedding Disposal:
[X] regular waste
[ ] autoclave then treat as regular waste
[ ] radioactive decay [ ] other: ____________________
[ ] autoclave [x] bleach [x] Spor-
[x] quat. ammonia compound [ ] regular cage wash
Surface and Equipment Decontamination procedure
[x] any of the above marked options
Note: avoid using bleach on stainless steel surfaces
[ ] room pressurization negative to corridor
[X] Class II biological safety cabinet used for cage changes
Animal Handling Precautions:
and animal handling (req. for BSL 2). Chemical fume hoods
may also be acceptable®.
Complete Freund’s Adjuvant” (CFA) contains heat-killed
mycobacteria, which is a primary agent responsible for
stimulating antibody production.
Eye and skin exposure to CFA via splashing may lead to
serious health complications. Occupational exposures of
CFA via accidental splashing into the unprotected eyes have
reportedly produced symptoms including severe ocular
irritation, scar tissue formation, and temporary/permanent
vision impairment. Limited available literature indicates
possible concerns including hypersensitivity and
interference with Mantoux test (tuberculin test) following
repeated skin exposure.
Additional Precautions: (e.g.: immunizations, restricted access,
special decontamination, specific waste disposal, etc.) There is less information on CFA being transmitted via dry
aerosol (dust). Proper eye and skin precautions should be
Bedding must be collected as a hazardous waste for the first
7 days after CFA injection. Contaminated bedding must be
emptied from cages using a Class I dump station or Class II
biosafety cabinet. Waste is placed in a yellow chemical
hazards bag for disposal by OEHS.
After this first cage change there is no need for further
special precautions to be taken regarding the animals or the
cages as long as the animals have not received additional
®Check with the PI first to make sure if sterile conditions must be maintained before using a chemical fume hood for cage
**NOTE: Individual cages must be identified with the hazardous agent, date of administration and appropriate hazard label
(chemical hazard sticker).
^Bags containing carcasses/ tissue must be labeled or tagged with the PI name and IACUC protocol number.
1. My staff and I will comply with all standards for animal care and investigation established in the Guide for the Care and Use of
Laboratory Animals and the Federal Animal Welfare Act, and will follow all policies established by the University to assure that
these standards are met.
2. I assume responsibility for the work described here.
3. All individuals working with the animals on this protocol are qualified by virtue of training or experience to perform proper
handling, experimental, and restraint techniques required for the species to be used.
4. I recognize my responsibility to identify occupational health hazards related to this protocol including identifying hazards,
providing the necessary training for those involved, and supplying the appropriate protective clothing and equipment to
minimize the risks.
5. This research does not represent unnecessary duplication of previous experiments.
6. I realize that failure to adhere to policies related to animal care and use may result in suspension or revocation of permission
to perform animal research in Wayne State University facilities.
Signature of Principal Investigator
(must be original; no copies, image files, etc.)
Approval Date (to be filled in by