Study Guide – Lectures 15-16
Reading: TEXT, Chapter 9
Background for lecture: DH Griffin. 1994. Fungal Physiology. Chapter 4
(Growth) and Chapter 5 (Chemical Requirements for Growth). Wiley-Liss,
Objectives Know the nutrients essential for fungal growth and from this, what you
put in fungal growth media, be it complex, defined, or minimal. Know the ways
that fungal growth is measured and be able to compare the good and bad
features of each method. What are R, C, and S selected fungi?
1. What nutrients must be supplied in a minimal medium? For what sorts of
experiments would you need to use minimal medium?
2. We have been using PDA, rose bengal agar, and MDA. Are these complex,
defined or minimal media? Will we isolate all soil fungi using rose bengal agar?
3. Draw three graphs: a) growth from a race tube experiment, b) dry weight from
an experiment performed in broth culture, c) linear growth rate.
4. Which methods for measuring growth are Terminal? If we are doing an
experiment that takes a week, but requires daily terminal measurements of
growth, how do we set up the experiment?
5. What are Kleb’s principles? If you want to induce sporulation in a laboratory
culture, i.e. cause condia to form or cause ascomata or basidiomata to form,
what general procedures could you use?
Keywords: broth, liquid culture, medium, selective medium, complex, defined and
minimal media, planktonic, structured, batch culture, continuous culture,
autoclave, sterilization, chelator, diurnal vs circadian periodicity, linear growth,
exponential growth, growth rate.