CIHR STRATEGIC TRAINING PROGRAM

							CIHR STRATEGIC TRAINING PROGRAM.
“Innovative Technologies in Multidisciplinary Health Research Training”

Intermediate and advanced course (April 28 to May 10, 2008).
NOTE: The program is flexible and some changes can be made (coffee breaks will
be included when possible).

             Monday, April 28.
9.00-10.00 Welcome/ distribution of Questionnaire #1
           Participant’s Introduction: where do you come from, what is your area
           of expertise?
           Introduction and overview.
           Dr. Sabine Mai, Ph.D., Manitoba Institute of Cell Biology,
           Winnipeg, Canada

10.00-12.00 Lecture 1: 1) “Intro to Fluorescence Spectroscopy and Instrument
                          Platforms”.
                       2) “Fluorescent Dyes and Probes”.
                       Iain Johnson, Invitrogen.

12.00-13.00 LUNCH

13.00-16.00 Lecture 2: “Ethical Conduct for Research Involving Humans”.
                        Dr. Catalena Birek, University of Manitoba
            TUTORIAL: Introduction to: The Canadian Tri-Council Policy
                          Statement: Ethical Conduct for Research Involving
                          Humans. (see link “doc#1” at “Papers and reading
                          materials for Tutorials and Journal Clubs”)

               Tuesday, April 29.
9.00-12.00 Lecture 3: “Functional genomics”.
                     Dr. Geoff Hicks, Ph.D., and Dr. Songyan Liu, Ph.D., Manitoba
                     Institute of Cell Biology, Winnipeg, Canada.
                    (Large scale functional analysis and annotation of the
                    mammalian genome. Understanding sequence analysis tools for
                    mining the genome. Database management).

12.00-12.45 LUNCH

13.00-17.00 Lecture 3: “Functional genomics”. Continued
                     Dr. Geoff Hicks, Ph.D., and Dr. Songyan Liu, Ph.D.,
                     Manitoba Institute of Cell Biology, Winnipeg, Canada.
                     (The single query. Functional annotation. Analysis of
                     large sets of sequence data).
                  Wednesday, April 30.
9.00-10.30 Lecture 4: “From the conception of the PRINS to its coronation”.
                       Dr. Regen Drouin, M.D., Ph.D., Service of Genetics, Dep of
                       Pediatrics, Faculty of Medicine and Health Sciences,
                       University of Sherbrooke, Quebec.

10.30-12.00 Lecture 5: “Identification and quantification of fetal cells in maternal
                        blood using molecular cytogenetic techniques”.
                        Dr. Regen Drouin, M.D., Ph.D., Service of Genetics, Dep of
                        Pediatrics, Faculty of Medicine and Health Sciences,
                        University of Sherbrooke, Quebec.

12.00-13.00 LUNCH

13.00-14.00 Lecture 6: “Chromatin immunoprecipitation assays for determining
                        assocation of proteins with specific genomic sequences”.
                        Dr. Jim Davie, Ph.D., Manitoba Institute of Cell Biology,
                        Winnipeg, Canada.

14.00-15.00 Lecture 7: “High resolution multi-color FISH and banding analysis”.
                        Dr. Ulrich Klingbeil. Ph.D., MetaSystems Group Inc.,
                        Boston. USA.

15.00-16.00 Lecture 8: “Automatic slide scanning”.
                        Dr. Ulrich Klingbeil. Ph.D., MetaSystems Group Inc.,
                        Boston. USA.

16.00-18.00 m-BAND imaging and analysis

                   Thursday, May 1.
8.30-10.30 m-BAND imaging and analysis


10.30-12.00 Lecture 9: “Introduction to optics: Waves, Rays, and Optical Imaging
                       Systems”.
                       P. Jansson, Ph.D., Hockessin, Delaware, USA.

12.00-12.30 LUNCH

12.30-14.00 Lecture 10: “Three-dimensional reconstruction of fluorescent objects
                        by deconvolution”.
                        P. Jansson, Ph.D., Hockessin, Delaware, USA.
14.00-15.00 Lecture 11: “Quantitative Image Processing: Theory”.
                        Bart Vermolen, M.Sc., University of Delft, The Netherlands.


15.00-16.00 Lecture 12: “Quantitative Image Processing: Practice”
                        Bart Vermolen, M.Sc., University of Delft, The Netherlands.

16.00-17.00 Lecture 13: “Electron microscopy – a tool for nano-imaging in cell
                        Biology”.
                        Dr. Sabine Hombach-Klonisch, Ph.D., Depts. of Human
                        Anatomy and Cell Science & Obstetrics and Gynecology,
                        University of Manitoba.

                      Friday, May 2.
9.00-10.00 Lecture 14: “Imaging tissue with IR and Raman microspectroscopy”.
                      Dr. Kathleen Gough, Ph. D., Department of Chemistry,
                      University of Manitoba

10.00-13.00 Lecture 15: “Advanced Imaging Methods”.
                        Dr. Yuval Garini, Ph. D., Physics Department,
                        Bar-Ilan University, Israel.

13.00-13.30 LUNCH

13.30-15.00 Lecture 16: “Applications and New Developments of Digital and
                         Spectral Imaging Technologies in Cytogenetic diagnostics
                         and research”.
                         Dr. Michael Koehler, Ph. D., Applied Spectral Imaging


15.00-18.00 SKY hybridization, day 1.

                      Saturday, May 3.
9.00-15.00 Q-FISH hybridization.

                      Sunday, May 4.
Free day
                       Monday, May 5.

8.30-12.00 SKY hybridization, day 2 (washes).
           During one of the antibody incubation steps:
           Lecture 17: “C-Myc Dependent Genomic Instability”
                       Dr. Sabine Mai, Ph.D., Manitoba Institute of Cell Biology,
                       Winnipeg, Canada

12.00-12.30 LUNCH

13.00-15.00 3D imaging at the Genomic Centre.

15.00-17.00 Field Trip to the Virtual Reality Centre.

                         Tuesday, May 6.
9.00-10.30 Lecture 18: “New microarray applications and validation of
                        microarray results”
                        Daniel Clutter, Ph.D., National Sales Director,
                        Roche NimbleGen

10.30-12.00 Lecture 19: “Multi-dimensional analysis of tumor genomes”.
                       Dr. Wan Lam, Ph.D., Clinical Professor, Pathology,
                       University of British Columbia and Senior Scientist, Cancer
                       Genetics and Developmental Biology, British Columbia
                       Cancer Agency

12.00-13.00 LUNCH

13.00-16.00 Imaging at the Genomic Centre

                          Wednesday, May 7.
9.00-10.30 Imaging at the Genomic Centre

10:30-12:00 Lecture 20: part 1: “Overview and New Applications of the Affymetrix
                       GeneChip Technology: the importance of context”

                        part 2: “Leading the Way in Genetic Discovery: Affymetrix
                       Tools for DNA Analysis”
                       Marco Raposo, Field Applications Specialist,
                       AFFYMETRIX, INC.
                       Eric Leblond, Senior Account Manager, AFFYMETRIX,
                       INC. (see content of lectures in “doc#2” at “Papers and
                       reading materials for Tutorials and Journal Clubs”)
12.00-13.00 LUNCH


13.00-15.00 Lecture 21: “Profiling Expression of Endogenous MicroRNAs”
                     Dr. Jeffrey P Johnston, Ph.D., Technology Sales Specialist
                     Gene Expression Profiling. Invitrogen Corporation.

15.00-17.00 Imaging at the Genomic Centre

                        Thursday, May 8.
9.00-10.30 Lecture 22: “Tumor Banks and Tissue Arrays in Cancer Research”.
                  Dr. Peter Watson, Director, TTR, Deeley Research Centre, BC
                  Cancer Agency, Vancouver Island Center, Victoria, BC and the
                  Manitoba Institute of Cell Biology, Department of Pathology,
                  Faculty of Medicine, University of Manitoba.

10.30-12.00 Lecture 23: “What has Fanconi Anemia taught us about breast
                      cancer?”
                      Dr Marc Tischkowitz, MD, PhD, Departments of Human
                      Genetics, Oncology and Medicine, McGill University, Jewish
                      General Hospital, Montreal

12.00-13.00 LUNCH

13.00-17.00 Imaging at the Genomic Centre

                       Friday, May 9.

8.30-12.00 Lecture 24: “Empowering Genomic Discoveries with the Agilent
                       Microarray Platform”.
                       Mona Prasad M.Sc, MBA, Agilent Technologies, Life Science
                      & Chemical Analysis, Product Specialist Integrated Biology
                       Solutions. (see content of lecture in “doc#2” at “Papers and
                       reading materials for Tutorials and Journal Clubs”)

12.00-13.00 LUNCH


13.00-14.30 Lecture 25: “Genomic deletions, duplications, and amplifications in
                        cancer-detection approaches, causes and clinical
                         consequences”
                       J. Squire, Ph.D., Ontario Cancer Institute, Toronto, Canada.

BREAK 14.30-14.45
14.45-16.15 Lecture 26: “Oncogenomics of prostate cancer- an example of
                        bench-to-bedside research”.
                        J. Squire, Ph.D., Ontario Cancer Institute, Toronto,
                        Canada.

                      Saturday, May 19.
9.00-14.00 Discussion of representative images (Q-FISH, m-BAND and SKY)
           from each group.
           Distribution of Questionnaire #2.

~14.00 Farewell Lunch