Celery Cell Organelles
1. Place a small slice of celery stalk on a blank slide.
2. Place one drop of 1% sucrose solution on the celery
3. Place the cover slip on top of the dyed celery at a 45-degree angle and lower.
4. Find the celery specimen under lowest power.
5. Draw what you see below. Try to find and label the cell wall, nucleus, chloroplasts,
mitochondria, and cytoplasm
6. Mitochondria and Chloroplasts are better seen with dye. Place one drop of Janus Green
Solution on the celery
7. Carefully place a tissue on the edge of the slide to remove excess dye and to draw the
solution across the specimen
8. Add anything new to the drawing above and label with a star to show that is was seen
with the dye
Using the class textbooks, fill out this T-chart
Section Heading Important information from the section
Reaction in the
Reaction in the
Light and Pigments
Reactions in the
1. Where were the majority of the mitochondria you observed located? Chloroplasts?
2. Why do you think the mitochondria were in those locations? Chloroplasts?
3. Do you think a skin or a muscle cell would contain more mitochondria? Why?
4. Do you think a leaf or a root cell would contain more chloroplasts? Why?
5. What do you think you would see if we didn’t use the sucrose solution? Why?
What claims can you now What evidence to you have What is the reasoning leads you
make about cells? to support those claims? from the evidence to the claim?
Teacher Note: Replace the book notes with whatever form of notes from your own textbook or
video to allow students to take notes on the function of chloroplasts and mitochondria
You can also add more inquiry to this lab by allowing students to experiment with different
concentrations of sucrose. I haven’t tried it yet, but it has come up as an interesting thing to
experiment with. You or the students may think of other things to test, like other forms of