rspb20070921supp3

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					                                                       Supplementary Tables 1-5

Table 1. Physio-chemical parameters in the East Bay and Pensacola Bay during sampling period in October 6-9, 2003.
Station               N1              N2                 TR                    H4               H3              H2            H1
Location          N 30o22.863’    N 30o23.245’      N 30o24.260’           N 30o26.084’   N 30o25.690’    N 30o26.583’   N 30o28.812’
                  W 87o15.149’    W 87o12.715’      W 87o06.996’           W 87o05.038’   W 87o01.041’    W 86o58.275’   W 87o01.985’

Bottom DO (mg/L) 4.62                 5.52              1.2-4.8                  3.20             1.88        1.35          1.32

Temperature (oC) 25.23                24.81             25.05                   25.05             24.79      24.86         24.56

Bottom salinity     31.05             30.88             29.85                    29.85            24.69       21.78         24.26
  (ppm)

   pH                7.94             8.07               7.98                     7.98             8.05           7.12       8.16

Depth (m)         9-10              9-10                5.5                       4.8              4.0            3.0        3.0
DO, dissolved oxygen; N, normoxic site; TR, transition site; H, hypoxic site.



Table 2. Physio-chemical parameters in the East Bay and Pensacola Bay during sampling period in November 3-5, 2003.
Station               N1             N2                 TR                     H4              H3             H2             H1
Location              o
                  N 30 22.823’    N 30o22.630’      N 30 24.197’
                                                         o
                                                                           N 30o26.029’   N 30 25.018’
                                                                                              o
                                                                                                          N 30 26.583’ N 30 28.806’
                                                                                                              o            o

                  W 87o15.060’    W 87o12.427’      W 87o06.859’           W 87o04.668’   W 87o01.032’    W 86o58.275’ W 87o02.009’

Bottom DO (mg/L) 6.65                 7.03              6.68                    4.72          2.93          2.22         3.54

Temperature (oC) 23.72               23.69             23.59                    23.78        23.83         24.12         23.99

Bottom salinity      32.09            32.21            31.29                    25.52        25.12         22.50          22.66
  (ppm)

   pH                8.14             8.18              8.12                    7.41          7.26           7.01         7.22

Depth (m)         9-10              9-10                5.5                   4.0             3.5            2.9          2.5
DO, dissolved oxygen, N, normoxic site; TR, transition site; H, hypoxic site.
Table 3. Physio-chemical parameters in the Pensacola Bay from April to November, 20031.
Sampling station: P06- (4.0 km west north west from TR site)
Location: N 30o24.91’, W 87o08.94’

Date                       April 16     May 6          June 4      July 15        August 12          September 23        October 22           November 21

Bottom DO                     3.97       1.09           3.22        2.99           0.99                  4.27                4.27                5.98
 (mg/L)

Water Temperature            16.68      22.63          24.55       28.50          27.58                  28.4               24.61                21.4
    (oC)

Bottom salinity              28.03      29.73          32.08       30.62           30.86                26.73               28.42               29.92
  (ppm)

  Depth                       5.5       5.5            5.25         6.0            5.25                  5.25                5.25                5.5
  (m)

Sampling station: P07- (2.4 km north from N2 site, 3.2 km east from N1 site)
Location: N 30o23.06’, W 87o12.59’

Date                       April 16     May 6          June 4      July 15        August 12          September 23        October 22           November 21

Bottom DO                    5.54       3.95            4.21        4.72            4.42                4.64                5.25                 5.62
 (mg/L)

Water Temperature          19.36        23.41         23.46        28.5             27.98               28.4                24.7                21.28
    (oC)

Bottom salinity            33.42       31.40          33.30       32.18            32.29               28.99                30.21               30.58
  (ppm)

   Depth                     10.25        11.25           10.25       10.0              8.75            10.25                10.25                 9.0
   (m)
1
  Physio-chemical parameters were collected and generously provided by Dr. James D. Hagy, US EPA, NHEERL/Gulf Ecology Division, Gulf Breeze, FL 32561, USA. DO,
dissolved oxygen.
Table 4. Physio-chemical parameters in the East Bay from July to November, 20031.
Sampling station: P12 (H1)*
Date                            July 17          July 24          August 22       August 29      October 31            November 3            November 6

Bottom DO (mg/L)                  0.13           0.16                0.2            0.28             0.17                 6.1                  7.13

Bottom salinity (ppm)             8.1            4.63              24.8             22.3            21.11               27.3                  27.04

Depth (m)                         3.0            2.66              2.6              2.83             2.52                 2.5                      2.7

Bottom hypoxic layer (m)           0.5            0.35             0.3              0.7              0.2                  0                          0
Sampling station: P13 (H2)*

Date                            July 17          July 24          August 22       August 29      October 31            November 3            November 6

Bottom DO (mg/L)                  0.2           0.21                0.28            0.26            0.16                  6.11                     6.7

Bottom salinity (ppm)            10.3           6.17                23.2            19.5             20.9                 23.0                  28.9

Depth (m)                        3.33           3.04                 3.0            3.42              2.91                3.02                     2.7

Bottom hypoxic layer (m)         0.55           0.3                  1.5             0.65              0.2                 0                         0
Sampling station: P14 (H3)*

Date                             July 17         July 24          August 22       August 29      October 31            November 3            November 631

Bottom DO (mg/L)                 0.18           0.23                 0.3             0.17             0.15                 0.12               6.7

Bottom salinity (ppm)            16.6           4.6                 25.4            25.4              22.9                 23.9               29.5

Depth (m)                        3.94           3.51                3.94             4.04                 3.04                3.52             3.2

Bottom hypoxic layer (m)          1.1           0.2                 1.6              0.9               0.2                    0.25             0
Sampling station: P15- (1.6 km south near H4 site, 3.2 km east from TR site)
Location: N 30o25.06’, W 87o05.32’

Date                                July 21                        August 25                       October                       November 6

Bottom DO (mg/L)                         0.39                            0.17                         -                              4.48

Botton salinity (ppm)                   24.0                          26.4                            -                              30.72

Depth (m)                                5.7                             6.8                          -                               6.9

Bottom hypoxic layer (m)                 2.0                             3.0                             -                               0
1
  Physio-chemical parameters were collected and generously provided by Dr. Richard A. Snyder, Center for Environmental Diagnostics and Bioremediation (CEDB), Department
of Biology, University of West Florida, Pensacola, FL 32514. *H1, H2, H3 indicate different hypoxic sampling sites. TR, transition site.
Table 5. Daily dissolved oxygen levels (mg/L) during a representative 2-week period in the laboratory experiment.
                                  Control                           Moderately hypoxic (DO 2.7 mg/L)                  Hypoxic (DO 1.7 mg/L)

                      Tank 1      Tank 2      Tank 3             Tank 1        Tank 2       Tank 3           Tank 1         Tank 2       Tank 3

November 09
       Morning         5.5         5.15         5.51              2.71          2.72          2.71             1.74          1.9          1.7
       Afternoon                                                  2.65          2.76          2.66             1.62          1.6          1.71
November 10
       Morning         5.37        5.3          5.3                2.74         2.62          2.87             1.84          1.83         1.66
       Afternoon                                                   2.7          2.7           2.7              1.7           1.66         1.61
November 11
       Morning         5.18        5.31         5.14               2.6          2.66          2.67            1.7            1.76         1.66
       Afternoon                                                   2.76         2.74          2.69            1.65           1.7          1.69
November 12
       Morning         5.22        5.5         5.31                2.89         2.82          2.94            1.74           1.75         1.76
       Afternoon                                                   2.7          2.87          2.94            1.64           1.92         1.79
November 13
       Morning         5.18        5.22        5.5                 2.62         2.47          2.74             1.74           1.85            1.98
       Afternoon                                                   2.59         2.5           2.56             1.67           1.76            1.75
November 14
       Morning         5.31        5.5         5.8                2.82          2.7           2.83             1.73           1.72            1.83
       Afternoon                                                  2.74          2.76          2.72             1.74           1.66            1.61
November 15
       Morning         5.15        5.15        5.25               2.64          2 .59         2.58             1.6            1.51            1.58
       Afternoon                                                  2.69          2.6           2.6              1.61           1.55            1.5
November 16
       Morning         5.3         5.3         5.2                2.56          2.68          2.5              1.6            1.57            1.42
       Afternoon                                                  2.9           2.7           2.53             1.77           1.72            1.82
November 17
       Morning         5.3         5.25        5.24               2.79          2.79          2.71             1.75           1.77            1.8
       Afternoon                                                  2.62          2.73          2.59             1.8            1.61            1.63
November 18
       Morning         5.4         5.3         5.32               2.67          2.64          2.7              1.69           1.72        1.69
       Afternoon                                                  2.66          2.62          2.67             1.68           1.75        1.72
November 19
       Morning         5.4         5.5         5.26               2.73          2.71          2.71             1.72          1.86             1.82
       Evening                                                    2.62          2.68          2.72             1.74          1.75             1.72
November 20
       Morning         5.36        5.25         5.4                2.71         2.69           2.67            1.77           1.68            1.67
       Afternoon                                                   2.66         2.59           2.6             1.66           1.71            1.55
November 21
       Morning         5.34        5.22         5.36               2.77         2.68          2.75             1.73           1.7             1.74
       Afternoon                                                   2.74         2.69          2.72             1.66           1.71            1.68
November 22
       Morning         5.38        5.42        5.43               2.8           2.7           2.87             1.74           1.7             2.1
       Afternoon                                                  2.69          2.72          2.68             1.77           1.85            1.9

				
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