Docstoc

물품구매명세

Document Sample
물품구매명세 Powered By Docstoc
					                                                           물품구매요구내역
                품           목                        규    격         수   량   단   가   금   액                  비고

 1 PCR DIG labeling mix, Roche                        2×250㎕        1 EA                    Roche Cat. No. 1 585 550

 2 DIG Wash and Block buffer set, Roche                       set   1 Set                   Roche Cat. No. 1 585 762

 3 Anti-Digoxigenin-AP,Fab fragments, Roche        150U(200㎕)       1 EA                    Roche Cat No. 1 093 274

 4 Gene Frame, Abgene, AB-0577                             65㎕      1 EA                    ABgene,  Cat. No. AB-0577

 5 Gene Frame, ABgene, AB-0578                            125㎕      1 EA                    ABgene,  Cat. No. AB-0578

 6 Dextran sulfate granule 50%, Sigma                     100g      1 EA                    SigmaD8906

 7 Yeast tRNA, Sigma                                 9.3mg/ml       1 EA                    Sigma No. R 8508

 8 Silane treated slides, Sigma                          72each     5 EA                    Sigma S4651

 9 Bismarck Brown Y, Sigma                                100g      1 EA                    Sigma B2759

10 BCIP, Roche                                                      1 EA                    Roche 1383213

11 NBT, Roche                                                       1 EA                    Roche 1383221

12 Coplin Jar, 스크류캡                                       5매입       15 EA

13 PCR Dig probe synthesis kit, Roche                         kit   1 EA                    Roche 1-636-090

14 High Pure PCR Template Preparation Kit, Roche              kit   1 EA                    Roche1 796 828

15 Formamide, Sigma                                       250g      1 EA                    Sigma F7508

16 SSC, USB, ultrapure MB grade, 0.2㎛ filtered            500㎖      1 EA                    USB corporation 19629

17 Denhardt'solution 50×, Sigma                               5㎖    1 EA                    Sigma D2532

18 Proteinase k, Sigma                                   100mg      1 EA

19 Tris, Amresco                                            1kg     1 EA                    Amresco 0826

20 Water pH7.0 for chromatography, Merck                   2.5ℓ     2 EA

21 PCR Tube, Corning                                      0.2㎖      1 PK

22 Neutral Buffered Formalin, Sigma                           4ℓ    2 EA                    Sigma 103k4358

23 HISTOPLEX(조직보존통), starplex                               box     1 Box                   starplex scientific inc. 3653

24 락앤락, 폴리프로필렌                                262×109×50mm          2 EA

25 Poly Glove                                               pak     30 PK

26 Peptone water, Fluka                                   500g      1 EA                          Fluka cat no.08105


27 Canada Balsam, Fluka                                   100㎖      1 EA                          Fluka cat no.03984

28 Tryptic Soy Broth, Difco                               500g      1 EA

29 Nutrient broth, Difco                                  500g      1 EA

30 Plate count agar, Difco                                500g      1 EA

31 pb standard sol., Wako                                 100㎖      1 EA

32 Cd standard sol., Wako                                 100㎖      1 EA

33 Hg standard sol., Wako                                 100㎖      1 EA

34 Lugol solution., Fluka                                 100㎖      1 EA                             Fluka 62650

35 SILVER NITRATE SOLUTION 5%                                 1L    1 ea                             Fulka 00667

36 GIEMSA STAIN, MODIFIED SOLUTION                            1L    1 ea                             Fulka 48900


37 Phosphate buffer pH7.2                            12개/box        1 ea                             Sigma-P3288


38 HARRIS HEMATOXYLIN SOLUTION, MODIFIED                      1L    1 ea                             Sigma-HHS32


39 EOSIN Y SOLUTION ALCOHOLIC                                 1L    1 ea                            Sigma-HT110132
40 Acid Alcohol                                  100ml      1 ea                   Sigma-A3957

41 Fraser broth supplement, Merck             8vials×12     1 EA     Merck-110399

42 Oxford agar supplement, Merck                13vials     1 EA     Merck-107006

43 Sheep blood agar, Biomerieux              10ea/pack      1 EA

44 Motility test medium, Difco                       500g   1 EA

45 Mannitol, Merck                                   500g   1 EA     Merck-015982

46 Rhamnose, Merck                                    500   1 EA     Merck-104736

47 Xylose, Merck                                     100g   1 EA     Merck-108689

48 Tryptic soy broth, Merck                          500g   1 EA

49 50% Egg yolk, Difco                     12X10㎖/tube      1 EA

50 KIA, Merck                                        500g   1 EA

51 TSI, Merck                                        500g   1 EA

52 Simon´s citrate agar, Merck                       500g   1 EA

53 TCBS, Merck                                       500g   1 EA

54 VP 반유동배지, Merck                                   500g   1 EA

55 Purple broth, Merck                               500g   1 EA

56   O-NITROPHENYL B-D-GALACTOPYRANOSIDE             500g   1 EA     Sigma N1127

57 Rappaport-vassiliadis, Merck                      500g   1 EA

58 MacConkey agar, Merck                             500g   1 EA

59 Desoxycholate citrate agar, Merck                 500g   1 EA

60 BGLB, Merck                                       500g   1 EA

61 Endo agar, Merck                                  500g   1 EA

62 EMB agar, Merck                                   500g   1 EA

63 Nutrient broth, Merck                             500g   1 EA

64 Selenite F broth, Merck                           500g   1 EA

65 VRBD agar, Merck                                  500g   1 EA

66 초산납지, Sigma                                10×75㎜        2 EA

67 스토마커용 스텐드, S/T Wire                               10H    2 EA

68 도가니(Cover 포함), MT                             100㎖       20 EA

69 일반세균용 건조필름배지                             50plates/pk     5 PK

70 대장균군용 건조필름배지                             25plates/pk     5 PK

71 황색포도상구균용 건조필름배지                          25plates/pk     5 PK

72 질소증류장치                                  제작(PYREX)         1 set   첨부 : 도면

73 라텍스글러브                                   100ea/box       10 box   korea ace scientific corp. DGPF-M

74 PHOSPHATE BUFFERED SALINE DRY POWDER       12Pak          1 ea    Sigma-P3744

75 Primer set                               0.2umol/각       89 ea    첨부 : primer목록 1부

76   가스글루건(글루건용 본드 100개포함)                 KGG-2401          1 ea    코베아 KGG-2401

77 실리콘건(실리콘 10개포함)                         CNV-100           1 EA    TAJIMA CNV-100
tarplex scientific inc. 3653
                                                           Primer List
                                                                             Primer
            질병이름                                                                                                                     비고
                                     구                     분                                      디자인
                                                                  F                  5'-cgc agt caa ggc ctt gat gt-3'
     EHN(유행성조혈기괴사)                         PCR                                                                                       1
                                                                  R               5'-aaa gac ccg ttt tgc agc agc aaa c-3'
                                         Nested                  1-F                  5'-tca agg ggg gag tcc tcg a-3'
                                         RT-PCR                  1-R                  5'-cac cgt act ttg ctg cta c-3'
     IHN(전염성조혈기괴사)                                                                                                                   2
                                                                 2-F                 5'-ttc gca gat ccc aac aac aa-3'
                                                                 2-R                   5'-gcg cac agt gcc ttg gct-3'
                                   Semi nested RT-PCR           1-F1               5'-tct tgg agc caa ata gct car rtc-3'
                                   (Glycoprotein gene)           1-R2          5'-aga tgg tat gga ccc caa tac ath acn cay-3'
    SVC(잉어봄바이러스혈증)                                                                                                                   3
                                                                 2-F1              5'-tct tgg agc caa ata gct car rtc-3'
                                                                 2-R4              5'-ctg ggg tttc cnc ctc aaa gyt gy-3'
                                          RT-PCR                  F                     5'-ggg gac ccc aga ctg t-3'
                                           (OIE)                  R                    5'-tct ctg tca cct tga tcc-3'
  VHS(바이러스성출혈성패혈증)                                                                                                                   4
                                          (NFRDI                  F                  5'-gag aga act ggc cct gac tg-3'
                                         /CEFAS)                  R                  5'-atg atc cgt ctg gct gac tc-3'
                                            PCR                   F                  5'-tca cgg aaa tac gac atc ca-3'
      IPN(전염성췌장괴사)                                                                                                                   5
                                          (NFRDI)                 R                  5'-tgt tgg aat tga ctg ggt ga-3'
      ISA(전염성연어빈혈)                                                F                 5'-ggc tat cta cca tga acg aat c-3'
                                          RT-PCR                                                                                     6
                                                                  R                  5'-gcc aag tgt aag tag cac tcc-3'
                                           PCR                    F                  5'-cgg ggg caa tga cga cta ca-3'
   RSIVD    (참돔이리도바이러스병)                                                                                                             7
                                       (DNA poly.)                R                  5'-ccg cct gtg cct ttt ctg ga-3'
                                       Nested-PCR                1-P3             5'-agc tcc gca agg tga agg g-3'(75-93)
                                      (p57 protein)             1-M21     5'-aga tgg tat gga ccc caa tac ath acn cay-3'(438-458)
       BKD(세균성신장병)                                                                                                                   8
                                                                 2-P4         5'-att ctt cca ctt caa cag tac aag g-3'(95-119)
                                                                2-M38          5'-cat tat cgt tac acc cga aac c-3'(394-415)
                                           PCR                    F                    5'-cta ttg gag ggc agt ct-3'
 Gyrodactylosis(자이로닥틸루스증)                                                                                                            9
                                       (V4 region)                R                    5'-ctt ttc agg caa caa gg-3'
                                           PCR                   1-F                 5'-gac gac gcc gga gac ctt gtg-3'
                                         (NFRDI)                 1-R               5'-cac aag ttc agt ctg ttc ctc aac-3'
  KHD(잉어헤르페스바이러스병)                                                                                                                   10
                                                                 2-F                 5'-ctc gcc gag cag agg aag cg-3'
                                                                 2-R                 5'-cac atg ggc agt ccg ctg gc-3'
                                                               RA 5-1               5'-gtt gaa cgt gcc ttc agt tta c-3'
  Xenohaliotis californiensis              PCR                                                                                       11
                                                               RA 3-6              5'-act tgg act cat tca aaa gcg ga-3'
                                           PCR                   p35           5'-ac(ct)ta(ct)gtgtcagacaacaaata(ct)tacaaa-3'
                                                                                                    5'-
                                    (polyhedrin gene)            p36     gg(ct)gcgtc(gt)gg(ct)gcaaa(ct)tt(ct)tt(at)ac(ct)tt(ag)aa-
                                                                                                     3'
             Spherical                                        pA1                  5'-gtg ctg caa cga ctg gac tgt cc-3'
baculovirosis(구상바큘로바이러스증)                                                                                                            12
                                                              pA2                 5'-tga tcc tag gcg tgc ctg tat tgg g-3'
                  
                                       Nested PCR           MBV1.4F                  5'-cga ttc cat atc ggc cga ata-3'
                                    (plasmid p4Ec196)       MBV1.4R                  5'-ttg gca tgc act ccc tga gat-3'
                                                            MBV1.4NF                 5'-tcc aat cgc gtc tgc gat act-3'
                                                            MBV1.4NR                 5'-cgc taa tgg ggc aca agt ctc-3'

                                           PCR                   BPA                  5'-gat ctg caa gag gac aaa cc-3'

                                   (polyhedrine gene)            BPB                 5'-atc gct aag ctc tgg cat cc-3'
   Tetrahedral baculovirosis
                                                                 BPD                 5'-tgt tct cag cca ata cat cg-3'                13
     (사면바큘로바이러스증)
                                                                 BPE                 5‘-tac atc ttg gat gcc tct gc-3'
                                                                 BPF                 5'-tac cct gca ttc ctt gtc gc-3'
                                                                 BPG                 5'-atc ctg ttt cca agc tct gc-3'
                                                                 389F                5'-cgg aac aca acc cga ctt ta-3'
                                           PCR1
                                                                 389R                5'-ggc caa gac caa aat acg aa-3'
 IHHN(전염성 피하 및 조혈기 괴사)                                                                                                               14
                                                                77012F                 5'-atc ggt gca cta ctc gga-3'
                                           PCR2
                                                                77353R                 5'-tcg tac tgg ctg ttc atc-3'
                                                                 10F                 5'-ccg cta att tca aaa act acg-3'
                                           PCR
                                                                 144R                5'-aag gtg tta tgt cga gga agt-3'
                                                                 GY5               5'-gag ctg gaa ttc agt gag aga aca-3'
                                                                 GY1               5'-gac atc act cca gac aac atc tg-3'
        YHD(노란머리병)                                                                                                                   15
                                                                 GY4               5'-gtg aag tcc atg tgt gtg aga cg-3'
                                          RT-PCR
                                                                 GY2               5'-cat ctg tcc aga agg cgt cta tga-3'
                                                                  Y3               5'-acg ctc tgt gac aag cat gaa gtt-3'
                                                                  G6               5'-gta gta gag acg agt gac acc tat-3'
                                                               1-146F1             5'-act act aac ttc agc cta tct ag-3'
                                                               1-146R1            5'-taa tgc ggg tgt aat gtt ctt acg a-3'
                                       Nested PCR
                                                               2-146F2              5'-gta act gcc cct tcc atc tcc a-3'
         WSD(흰반점병)                                                                                                                   16
                                                               2-146R2              5'-tac ggc agc tgc tgc acc ttg t-3'
                                                                 143F              5'-tgc ctt atc agc tnt cga ttg tag-3'
                                 Decapod-specific primer
                                                                 145R              5'-ttc agn ttt gca acc ata ctt ccc-3'
                                                                 9195                  5'-tca atg aga gct tgg tcc-3'
  Taura syndrome(타우라증후군)                   PCR                                                                                       17
                                                                 9992                  5'-aag tag aca gcc gcg ctt-3'
                                                                  F                 5'-acg ttg gag gtt cgt cct tca g-3'
           NHP
                                           PCR                    R1                5'-tca ccc cct tgc ttc tca ttg t-3'              18
       (궤사성간췌장염)
                                                                  R2               5'-cca gtc atc acc ttt tct gtg gtc-3'
           IMN                                                    F                  5'-aac aca aaa tct gcc agc aa-3'
                                          RT-PCR                                                                                     19
        (전염성근괴사)
            IMN
                              RT-PCR                                                 19
      (전염성근괴사)                              R    5'-ccc aac cac cca aat tca ta-3'
            HRI                             F    5'-tga ggt aga gca agc caa ca-3'
                              RT-PCR                                                 20
 (넙치 랍도바이러스감염증)                             R    5'-aca tga ctg gcc gaa aag ag-3'
            MBI                             F    5'-gca cca cga agg tac gaa at-3'
                              RT-PCR                                                 21
 (해산 버나바이러스감염증)                             R    5'-gta cgt tgc cgt ttc ctg at-3'
            FII                             F    5'-gta cca ggg cca tgt gaa gt-3'
                                PCR                                                  22
(어류 이리도바이러스감염증)                             R    5'-gca caa tga gct tca ttc ca-3'
   Lymphocystis disease        PCR          F    5'-gac aca acc cgt tga ttc ca-3'
                                                                                     23
     (림포시스티스병)               (NFRDI)        R    5'-cag caa tac ccg gta aat cg-3'
                               RT-PCR       F     5'-ccc cga tca tca cca cga t-3'
      HDGC                  GCRV-874 S10    R     5'-cgc gtt cgc tga tgt aag g-3'
                                                                                     24
 (초어출혈성 바이러스병)                 RT-PCR       F     5'-agt tct caa agc tga gac ag-3'
                             GCRV-14 M6     R    5'-acg tgc gat tgg aag agc tt-3'
                             Nested        1-F   5'-cgg ata cgt tgt tgt tga cg-3'
 Viral encephalopathy and
                             RT-PCR        1-R   5'-caa cag gca gca gaa ttt ga-3'
        retinopathy                                                                  25
(바이러스성뇌질환및망막증)               (NFRDI)       2-F   5'-ctg gga cac gct gct aga at-3'
                                           2-R   5'-cga aac gtt gac cac atc ag-3'
      HPI                                   R3     5'-ggt gat gtg gag gag aga-3'
                                PCR                                                  26
(간췌장파보바이러스감염증)                              F4     5'-gta act atc gcc gcc acc-3'
          질소증류장치 도면




※ 버너부분은 히팅멘틀로 하여
  온도조절 가능하도록 제작
※ 초자기구는 PYREX제질로 제작
※ 도면 (1)로 제작
도면

				
DOCUMENT INFO
Shared By:
Categories:
Tags:
Stats:
views:214
posted:8/1/2012
language:Korean
pages:7