Cell - PowerPoint by liwenting

VIEWS: 3 PAGES: 39

									          The Cell

Observed by Light and Electron
         Microscopy
Overview of the Cellular
      Organelles
Light Microscopy – Hematoxylin & Eosin (H&E) Stain



 Basophilic
   (blue)
  Nuclei


Eosinophilic
    (red)
 cytoplasm
          Colorized low mag TEM of a cell


Red =
Mitos


Blue =
Ribos


Green =
 RER
          Colorized TEM of a cell nucleus


Arrow =
Nucleolus
Red =
Mitos
Green =
RER
Nuclear Structures
Low mag TEM of a nuclei
Nuclear pores (arrows)   Nuclear pores   (en face)
Colorized TEM of nuclear pores (blue) en face
 High mag TEM of a
     nucleolus


Pars fibrosa/granulosa
  (dense black) and
fibrillar center (arrow)
TEM of interphase nuclei and a mitotic cell with
     condensed chromosomes (arrow)
Cytoplasmic Structures
  Ribosomes, RER and
        Glogi
Ribosomes by light microscopy = basophilic
                cytoplasm
TEMs of free ribosomes – 25 nm granules – in
          clusters called polysomes
Colorized TEM of polysomes (yellow highlighted)
Low mag TEM of rough endoplasmic reticulum
                 (RER)
Colorized higher mag TEM of RER (green)
TEM of RER – arrows indicate cisternae
Low mag TEM of RER and Golgi (Go)
Colorized TEM of a Golgi system
TEM of a Golgi system
TEM of lysosomes (arrows) & a residual body




                                   Residual
                                    Body
Cytoplasmic
inclusions by Light
Microscopy
Basophilic granules
(top picture)




Eosinophilic granules
(bottom picture)
Mitochondria
Colorized TEM of Mitochrondria (red)
TEMs of Mitochrondrial shapes
   TEM of a
Mitochrondrion


  Arrows =
   cristae
The Cytoskeleton
Light microscopy of mitotic spindles = Microtubules
Colorized TEM of Cilia in cross section showing
                microtubules
Immunofluorescence of Microtubules in cultured
               cells (green)
TEM of Microfilaments (arrows)
Immunofluorescence of microfilament stress
          fibers in cultured cells
Cell Membrane
High mag TEM of two cell membranes
         (unit membrane)
Colorized TEM of two cell membranes
          (unit membrane)
TEMs of membrane
  specializations


 Top 4 = microvilli




Middle = Stereo cilia




   Bottom = Cilia
TEM of Cilia (C) with
 microtubular cores
 forming from basal
    bodies (BB)
 High mag TEM of
      Cilia with
microtubular cores
(Fil) with the basal
body (FiC) at top of
       image

								
To top